scholarly journals High-Yield, In Vitro Protein Expression Using a Continuous-Exchange, Coupled Transcription/Translation System

BioTechniques ◽  
2001 ◽  
Vol 31 (4) ◽  
pp. 948-953 ◽  
Author(s):  
G.A. Martin ◽  
R. Kawaguchi ◽  
Y. Lam ◽  
A. DeGiovanni ◽  
M. Fukushima ◽  
...  
Keyword(s):  
Protein Expression ◽  
High Yield ◽  
Translation System ◽  
In Vitro Protein Expression ◽  
Vitro Protein

A PMMA microfluidic droplet platform for in vitro protein expression using crude E. coli S30 extract

Lab on a Chip ◽  
2009 ◽  
Vol 9 (23) ◽  
pp. 3391 ◽  
Author(s):  
N. Wu ◽  
Y. Zhu ◽  
S. Brown ◽  
J. Oakeshott ◽  
T. S. Peat ◽  
...  
Keyword(s):  
Protein Expression ◽  
E Coli ◽  
S30 Extract ◽  
In Vitro Protein Expression ◽  
Vitro Protein

Proficient Target Selection in Structural Genomics by In Vitro Protein Expression on Gateway Recombination Plasmids

2002 ◽  
pp. 197-202 ◽  
Author(s):  
Vincent Monchois ◽  
Renaud Vincentelli ◽  
Céline Deregnaucourt ◽  
Chantal Abergel ◽  
Jean-Michel Claverie
Keyword(s):  
Protein Expression ◽  
Structural Genomics ◽  
Target Selection ◽  
In Vitro Protein Expression ◽  
Vitro Protein

scholarly journals Anionic Complex with Efficient Expression and Good Safety Profile for mRNA Delivery

Pharmaceutics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 126
Author(s):  
Eri Hamada ◽  
Tomoaki Kurosaki ◽  
Junya Hashizume ◽  
Hitomi Harasawa ◽  
Hiroo Nakagawa ◽  
...  
Keyword(s):  
Protein Expression ◽  
Messenger Rna ◽  
Charge Ratio ◽  
Attractive Alternative ◽  
Target Cells ◽  
Anionic Complex ◽  
In Vitro Protein Expression ◽  
Vitro Protein

We previously found that a complex comprising plasmid DNA (pDNA), polyethylenimine (PEI), and γ-polyglutamic acid (γ-PGA) had high transgene efficiency without cytotoxicity in vitro and in vivo. However, messenger RNA (mRNA) remains an attractive alternative to pDNA. In this study, we developed a safe and effective delivery system for mRNA to prevent its degradation and efficiently deliver it into target cells. Various cationic and anionic complexes were produced containing PEI, γ-PGA, and an mRNA encoding firefly luciferase. Their physicochemical properties and cytotoxicities were analyzed and the in vitro and in vivo protein expression were determined. The cationic mRNA/PEI complex showed high in vitro protein expression with strong cytotoxicity. The anionic complex was constructed as mRNA/PEI8/γ-PGA12 complex with a theoretical charge ratio of 1:8:12 based on the phosphate groups of the mRNA, nitrogen groups of PEI, and carboxylate groups of γ-PGA. It was stable and showed high in vitro protein expression without cytotoxicity. After intravenous administration of mRNA/PEI8/γ-PGA12 complex to mice, high protein expression was observed in the spleen and liver and slight expression was observed in the lung over 24 h. Thus, the newly constructed mRNA/PEI8/γ-PGA12 complex provides a safe and effective strategy for the delivery of mRNA.

scholarly journals Application of In Vitro Protein Expression to Human Prote

2002 ◽  
Vol 2 ◽  
pp. 73-74 ◽  
Author(s):  
Peter T. Beernink ◽  
Simone S. Krupka ◽  
Victoria Lao ◽  
George Martin ◽  
Matthew A. Coleman
Keyword(s):  
Protein Expression ◽  
In Vitro Protein Expression ◽  
Vitro Protein

Toxin Detection by a Miniaturized in Vitro Protein Expression Array

2005 ◽  
Vol 77 (17) ◽  
pp. 5494-5500 ◽  
Author(s):  
Qian Mei ◽  
Carl K. Fredrickson ◽  
Shouguang Jin ◽  
Z. Hugh Fan
Keyword(s):  
Protein Expression ◽  
Expression Array ◽  
Toxin Detection ◽  
In Vitro Protein Expression ◽  
Vitro Protein

Construction of homo- and heteropolymers of plant ferritin subunits using an in vitro protein expression system

2007 ◽  
Vol 56 (2) ◽  
pp. 237-246 ◽  
Author(s):  
Taro Masuda ◽  
Fumiyuki Goto ◽  
Toshihiro Yoshihara ◽  
Toru Ezure ◽  
Takashi Suzuki ◽  
...  
Keyword(s):  
Protein Expression ◽  
Expression System ◽  
Protein Expression System ◽  
In Vitro Protein Expression ◽  
Vitro Protein
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