scholarly journals PCR-Based RNA Probes: A Quick and Sensitive Method to Improve Whole Mount Embryo In Situ Hybridizations

BioTechniques ◽  
2001 ◽  
Vol 30 (4) ◽  
pp. 768-774 ◽  
Author(s):  
Robert David ◽  
Doris Wedlich
Keyword(s):  
Rna Probes ◽  
Whole Mount ◽  
In Situ Hybridizations ◽  
Sensitive Method

Whole-Mount In Situ Hybridization of Short-Tailed Fruit Bat (Carollia perspicillata) Embryos with RNA Probes

2009 ◽  
Vol 2009 (3) ◽  
pp. pdb.prot5164-pdb.prot5164 ◽  
Author(s):  
J. J. Rasweiler ◽  
C. J. Cretekos ◽  
R. R. Behringer
Keyword(s):  
In Situ Hybridization ◽  
Carollia Perspicillata ◽  
Fruit Bat ◽  
Rna Probes ◽  
Whole Mount

scholarly journals Germline Transformation of Drosophila virilis With the Transposable Element mariner

Genetics ◽  
1996 ◽  
Vol 143 (1) ◽  
pp. 365-374 ◽  
Author(s):  
Allan R Lohe ◽  
Daniel L Hartl
Keyword(s):  
Transposable Element ◽  
Common Ancestor ◽  
Pcr Amplification ◽  
Drosophila Virilis ◽  
Cell Region ◽  
Diverse Species ◽  
Drosophila Mauritiana ◽  
Characteristic 2 ◽  
In Situ Hybridizations

Abstract An important goal in molecular genetics has been to identify a transposable element that might serve as an efficient transformation vector in diverse species of insects. The transposable element mariner occurs naturally in a wide variety of insects. Although virtually all mariner elements are nonfunctional, the Mosl element isolated from Drosophila mauritiana is functional. Mosl was injected into the pole-cell region of embryos of D. virilis, which last shared a common ancestor with D. mauritiana 40 million years ago. Mosl PCR fragments were detected in several pools of DNA from progeny of injected animals, and backcross lines were established. Because Go lines were pooled, possibly only one transformation event was actually obtained, yielding a minimum frequency of 4%. Mosl segregated in a Mendelian fashion, demonstrating chromosomal integration. The copy number increased by spontaneous mobilization. In situ hybridization confirmed multiple polymorphic locations of Mosl. Integration results in a characteristic 2-bp TA duplication. One Mosl element integrated into a tandem array of 370-bp repeats. Some copies may have integrated into heterochromatin, as evidenced by their ability to support PCR amplification despite absence of a signal in Southern and in situ hybridizations.

scholarly journals FISHing for chick genes: Triple-label whole-mount fluorescence in situ hybridization detects simultaneous and overlapping gene expression in avian embryos

2004 ◽  
Vol 229 (3) ◽  
pp. 651-657 ◽  
Author(s):  
Nathaniel Denkers ◽  
Pilar García-Villalba ◽  
Christopher K. Rodesch ◽  
Kandice R. Nielson ◽  
Teri Jo Mauch
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Avian Embryos ◽  
Overlapping Gene ◽  
Whole Mount
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