scholarly journals Chromosome Walking by Cloning of Distinct PCR Fragments

BioTechniques ◽  
2001 ◽  
Vol 30 (2) ◽  
pp. 248-249 ◽  
Author(s):  
Bernd Kneidinger ◽  
Michael Graninger ◽  
Paul Messner
Keyword(s):  
Chromosome Walking

PCR-based Chromosome Walking: The SFR 3 Case

2005 ◽  
Vol 9 (1) ◽  
pp. 164-169 ◽  
Author(s):  
Azura Amid . ◽  
Garry J. Warren . ◽  
Peter M. Bramley .
Keyword(s):  
Chromosome Walking

Three unlinked gene clusters are involved in clavam metabolite biosynthesis in Streptomyces clavuligerus

2004 ◽  
Vol 50 (10) ◽  
pp. 803-810 ◽  
Author(s):  
Kapil Tahlan ◽  
Hyeon Ung Park ◽  
Susan E Jensen
Keyword(s):  
Clavulanic Acid ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Biosynthetic Pathway ◽  
Gene Clusters ◽  
Streptomyces Clavuligerus ◽  
Pulsed Field Gel Electrophoresis ◽  
Single Family ◽  
Chromosome Walking ◽  
Restriction Fragments

In Streptomyces clavuligerus, three groups of genes are known to be involved in the biosynthesis of the clavam metabolites. Since antibiotic biosynthetic genes are invariably clustered on the chromosome in prokaryotes, chromosome walking was undertaken in an attempt to show that the three groups of clavam genes would resolve into a single super-cluster when analyzed at larger scale. However, no evidence of linkage between the three groups was obtained. Furthermore, Southern analysis of macro-restriction fragments of genomic DNA separated by pulsed-field gel electrophoresis also indicated that the three groups of genes are not linked. Despite the structural and biosynthetic relatedness of the clavam metabolites, our results suggest that the genes involved in their production lie in three unlinked gene clusters. We believe that this represents the first instance in bacteria of genes involved in the biosynthesis of a single family of antibiotics sharing a common biosynthetic pathway and yet residing in three separate locations on the chromosome.Key words: Streptomyces, clavulanic acid, clavams, paralogues, gene clusters.

Anchored PCR (A-PCR): A new method for chromosome walking

2004 ◽  
Vol 49 (16) ◽  
pp. 1772-1774 ◽  
Author(s):  
Bojun Chen ◽  
Chao Sun ◽  
Yong Wang ◽  
Yuanlei Hu ◽  
Zhongping Lin
Keyword(s):  
New Method ◽  
Chromosome Walking ◽  
Anchored Pcr
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