scholarly journals Heteroduplex Resolution Using T7 Endonuclease I in Microbial Community Analyses

BioTechniques ◽  
2000 ◽  
Vol 28 (4) ◽  
pp. 676-681 ◽  
Author(s):  
Jennifer L. Lowell ◽  
Donald A. Klein
Keyword(s):  
Microbial Community ◽  
Microbial Community Analyses

scholarly journals Evaluation of DNA Extraction Methods of Mule Dung

2019 ◽  
Vol 4 (3) ◽  
pp. 170-174
Author(s):  
Rajesh Kumar Vaid ◽  
Taruna Anand ◽  
Priyanka Batra ◽  
Ram Avtar Legha ◽  
Bhupendra Nath Tripathi
Keyword(s):  
Microbial Community ◽  
Microbial Diversity ◽  
Dna Isolation ◽  
Community Analysis ◽  
Extraction Methods ◽  
Microbial Community Analysis ◽  
Animal Dung ◽  
Microbial Community Analyses ◽  
Dna Extraction Methods ◽  
Selection Of

DNA isolation is a critical step in microbial community analysis of animal dung. DNA isolation from mule dung is challenging due to microbial diversity, composition and chemical nature of mule dung. Therefore, selection of an appropriate DNA isolation method is important to analyse the complete microbial diversity. In the current study, we evaluated the DNA isolation from mule dung samples (n=11) using QiAmp Mini stool kit as per manufacturer’s procedure with modifications. The results suggest that modifications in proprietary column based method improved the DNA quality and quantity suitable for mule dung microbial community analyses.

scholarly journals Microbial Community Analyses Inform Geochemical Reaction Network Models for Predicting Pathways of Greenhouse Gas Production

2019 ◽  
Vol 7 ◽  
Author(s):  
Rachel M. Wilson ◽  
Rebecca B. Neumann ◽  
Kelsey B. Crossen ◽  
Nicole M. Raab ◽  
Suzanne B. Hodgkins ◽  
...  
Keyword(s):  
Microbial Community ◽  
Greenhouse Gas ◽  
Reaction Network ◽  
Network Models ◽  
Gas Production ◽  
Microbial Community Analyses ◽  
Geochemical Reaction

Metagenome analyses reveal the role of Clostridium perfringens in alfalfa silage anaerobic deterioration

2020 ◽  
Vol 367 (8) ◽  
Author(s):  
Mingli Zheng ◽  
Dongze Niu ◽  
Di Jiang ◽  
Rongrong Li ◽  
Lin Meng ◽  
...  
Keyword(s):  
Microbial Community ◽  
Bacterial Community ◽  
Clostridium Perfringens ◽  
Relative Abundance ◽  
Dominant Role ◽  
Ammonia Nitrogen ◽  
Fermentation Products ◽  
Microbial Community Analyses ◽  
Alfalfa Silage

ABSTRACT The clostridial fermentation caused by the outgrowth of Clostridia was mainly responsible for the silage anaerobic deterioration. Our previous results showed that Clostridium perfringens dominated the clostridial community in poor-fermented alfalfa silage. This study was conducted to further examine the role of C. perfringens in silage anaerobic deterioration through fermentation products and the microbial community analyses. Direct-cut alfalfa was ensiled with C. perfringens contamination (CKC) or with the addition of Lactobacillus plantarum, sucrose and C. perfringens (LSC). Contamination with C. perfringens enhanced the clostridial fermentation in CKC silage, as indicated by high contents of butyric acid, ammonia nitrogen and Clostridia, while LSC silage was well preserved. The genera Bifidobacterium, Garciella and Clostridium dominated the bacterial community in CKC silage, while predominate genus was replaced by Lactobacillus in LSC silage. The clostridial community in CKC silage was dominated by Garciella sp. (26.9 to 58.1%) and C. tyrobutyricum (24.4 to 48.6%), while the relative abundance of C. perfringens was below 5.0%. Therefore, the effect of Clostridia contamination on ensiling fermentation was dependent on the ensilability of the silage material. Garciella sp. and C. tyrobutyricum, rather than C. perfringens, played dominant role in the clostridial fermentation in CKC silage.

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