scholarly journals Combined in vitro transcription and reverse transcription to amplify and label complex synthetic oligonucleotide probe libraries

BioTechniques ◽  
2015 ◽  
Vol 58 (6) ◽  
Author(s):  
Yusuf Murgha ◽  
Brian Beliveau ◽  
Kassandra Semrau ◽  
Donald Schwartz ◽  
Chao-ting Wu ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Oligonucleotide Probe ◽  
In Vitro Transcription ◽  
Synthetic Oligonucleotide ◽  
Synthetic Oligonucleotide Probe

Cloning the gene for the heat shock response positiwe regulator (sigma 32 homolog) from Pseudomonas aeruginosa

1995 ◽  
Vol 41 (1) ◽  
pp. 75-87 ◽  
Author(s):  
Zerlina M. Naczynski ◽  
Andrew M. Kropinski ◽  
Chris Mueller
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Heat Shock ◽  
Sigma Factor ◽  
Oligonucleotide Probe ◽  
In Vitro Transcription ◽  
Translation System ◽  
Amino Acid Homology ◽  
E Coli ◽  
Transcriptional Start Sites

A 31 base pair synthetic oligonucleotide based on the genes for the Escherichia coli heat shock sigma factor (rpoH) and the Pseudomonas aeruginosa housekeeping sigma factor (rpoD) was employed in conjunction with the Tanaka et al. (K. Tanaka, T. Shiina, and H. Takahashi, 1988. Science (Washington, D.C.), 242: 1040–1042) RpoD box probe to identify the location of the rpoH gene in P. aeruginosa genomic digests. This gene was cloned into plasmid pGEM3Z(f+), sequenced, and found to share 67% nucleotide identity and 77% amino acid homology with the rpoH gene and its product (σ32) of E. coli. The plasmid containing the rpoH gene complemented the function of σ32 in an E. coli rpoH deletion mutant. Furthermore, this plasmid directed the synthesis of a 32-kDa protein in an E. coli S-30 in vitro transcription–translation system. Primer extension studies were used to identify the transcriptional start sites under control and heat-stressed (45 and 50 °C) conditions. Two promoter sites were identified having sequence homology to the E. coli σ70 and σ24 consensus sequences.Key words: heat shock, Pseudomonas aeruginosa, sigma factor, transcription, oligonucleotide probe.

Evaluation of a Synthetic Oligonucleotide Probe for Diagnosis of Plasmodium falciparum Infections

1986 ◽  
Vol 35 (5) ◽  
pp. 912-920 ◽  
Author(s):  
Cathleen M. Mucenski ◽  
John H. Cross ◽  
George Watt ◽  
David Walliker ◽  
Olegario R. Majam ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Oligonucleotide Probe ◽  
Synthetic Oligonucleotide ◽  
Synthetic Oligonucleotide Probe

scholarly journals Cloning of the Vibrio harveyi luciferase genes: use of a synthetic oligonucleotide probe.

1983 ◽  
Vol 80 (1) ◽  
pp. 120-123 ◽  
Author(s):  
D. H. Cohn ◽  
R. C. Ogden ◽  
J. N. Abelson ◽  
T. O. Baldwin ◽  
K. H. Nealson ◽  
...  
Keyword(s):  
Oligonucleotide Probe ◽  
Vibrio Harveyi ◽  
Synthetic Oligonucleotide ◽  
Synthetic Oligonucleotide Probe

Mutagenic properties of oxidized GTP and ATP in in vitro transcription-reverse transcription

2006 ◽  
Vol 50 (1) ◽  
pp. 99-100
Author(s):  
Hiroyuki Kamiya ◽  
Akihiro Suzuki ◽  
Kazuaki Kawai ◽  
Hiroshi Kasai ◽  
Hideyoshi Harashima
Keyword(s):  
Reverse Transcription ◽  
In Vitro Transcription
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