scholarly journals A simple modification to the luminometric methylation assay to control for the effects of DNA fragmentation

BioTechniques ◽  
2015 ◽  
Vol 58 (5) ◽  
Author(s):  
Elif Aysimi Duman ◽  
Skirmantas Kriaucionis ◽  
John J. Dunn ◽  
Eli Hatchwell
Keyword(s):  
Dna Fragmentation ◽  
Simple Modification ◽  
Methylation Assay ◽  
Luminometric Methylation Assay

LUMA (LUminometric Methylation Assay)—A high throughput method to the analysis of genomic DNA methylation

2006 ◽  
Vol 312 (11) ◽  
pp. 1989-1995 ◽  
Author(s):  
Mohsen Karimi ◽  
Sofia Johansson ◽  
Dirk Stach ◽  
Martin Corcoran ◽  
Dan Grandér ◽  
...  
Keyword(s):  
Dna Methylation ◽  
High Throughput ◽  
Genomic Dna ◽  
Methylation Assay ◽  
High Throughput Method ◽  
Luminometric Methylation Assay

scholarly journals Mercury-associated DNA hypomethylation in polar bear brains via the LUminometric Methylation Assay: a sensitive method to study epigenetics in wildlife

2009 ◽  
Vol 19 (2) ◽  
pp. 307-314 ◽  
Author(s):  
J. RICHARD PILSNER ◽  
ALICIA L. LAZARUS ◽  
DONG-HA NAM ◽  
ROBERT J. LETCHER ◽  
CHRISTIAN SONNE ◽  
...  
Keyword(s):  
Polar Bear ◽  
Dna Hypomethylation ◽  
Methylation Assay ◽  
Luminometric Methylation Assay ◽  
Sensitive Method

DNA-fragmentation and expression of apoptosis-related proteins in experimentally denervated and reinnervated rat facial muscle

1997 ◽  
Vol 23 (3) ◽  
pp. 141-149 ◽  
Author(s):  
D. S. Tews ◽  
H. H. Goebel ◽  
I. Schneider ◽  
A. Gunkel ◽  
E. Stennert ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Facial Muscle ◽  
Related Proteins

Simple Modification Measurement of Photodegradability of Polymers by using Photo-induced Chemiluminescence Technique

2013 ◽  
Vol 10 (2) ◽  
pp. 51
Author(s):  
Siti Farhana Zakaria ◽  
Keith R Millington
Keyword(s):  
Free Radicals ◽  
Organic Materials ◽  
Oxidative Degradation ◽  
Working Life ◽  
Bimolecular Reaction ◽  
Synthetic Polymers ◽  
Accelerated Weathering ◽  
Simple Modification ◽  
Uv Absorbers ◽  
Irradiation Period

Polymers and organic materials that are exposed to sunlight undergo photooxidation, which leads to deterioration of their physical properties. To allow adequate performance under outdoor conditions, synthetic polymers require additives such as antioxidants and UV absorbers. A major problem with optimising polymer formulations to maximise their working life span is that accelerated weathering tests are empirical. The conditions differ significantly from real weathering situations, and samples require lengthy irradiation period. Degradation may not be apparent in the early stages of exposure, although this is when products such as hydroperoxides are formed which later cause acceleration of oxidation. A simple way of quantifying the number of free radicals presents in organic materials following exposure to light or heat is by measuring chemiluminescence (CL) emission. Most polymers emit CL when they undergo oxidative degradation, and it originates from the bimolecular reaction of macroperoxy radicals which creates an excited carbonyl.

Sign in / Sign up

Export Citation Format

Share Document