scholarly journals Automation of the cytokinesis-block micronucleus cytome assay by laser scanning cytometry and its potential application in radiation biodosimetry

BioTechniques ◽  
2014 ◽  
Vol 57 (6) ◽  
Author(s):  
Maxime François ◽  
Kevin Hochstenbach ◽  
Wayne Leifert ◽  
Michael Felix Fenech
Keyword(s):  
Potential Application ◽  
Laser Scanning ◽  
Laser Scanning Cytometry ◽  
Cytome Assay ◽  
Radiation Biodosimetry

Automation of the Buccal Micronucleus Cytome Assay Using Laser Scanning Cytometry

2011 ◽  
pp. 321-339 ◽  
Author(s):  
Wayne R. Leifert ◽  
Maxime François ◽  
Philip Thomas ◽  
Ed Luther ◽  
Elena Holden ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Laser Scanning Cytometry ◽  
Cytome Assay

Enumeration of Cryptosporidium oocysts from surface water concentrates by laser-scanning cytometry

2000 ◽  
Vol 41 (7) ◽  
pp. 197-202 ◽  
Author(s):  
F. Zanelli ◽  
B. Compagnon ◽  
J. C. Joret ◽  
M. R. de Roubin
Keyword(s):  
Surface Water ◽  
Water Samples ◽  
Laser Scanning ◽  
Immunomagnetic Separation ◽  
Entire Surface ◽  
Cryptosporidium Oocysts ◽  
Different Types ◽  
Laser Scanning Cytometry ◽  
Direct Microscopic Observation

The utilization of the ChemScan® RDI was tested for different types of water concentrates. Concentrates were prepared by cartridge filtration or flocculation, and analysed either without purification, or after Immunomagnetic separation (IMS) or flotation on percoll-sucrose gradients. Theenumeration of the oocysts was subsequently performed using the ChemScan® RDI Cryptosporidium application. Enumeration by direct microscopic observation of the entire surface of the membrane was carried out as a control, and recoveries were calculated as a ratio between the ChemScan® RDI result and the result obtained with direct microscopic enumeration. The Chemscan enumeration technique proved reliable, with recoveries yielding close to 100% in most cases (average 125%, range from 86 to 467%) for all the concentration/purification techniques tested. The quality of the antibodies was shown to be critical, with antibodies from some suppliers yielding recoveries a low as 10% in some cases. This difficulty could, however, be overcome by the utilization of the antibody provided by Chemunex. These data conclusively prove that laser scanning cytometry, which greatly facilitates the microscopic enumeration of Cryptosporidium oocysts from water samples and decreases the time of observation by four to six times, can be successfully applied to water concentrates prepared from a variety of concentration/purification techniques.

scholarly journals Laser scanning cytometry in the characterization of the proapoptotic effects of transiently transfected genes in cerebellar granule neurons

2006 ◽  
Vol 69A (11) ◽  
pp. 1114-1122 ◽  
Author(s):  
Brendan Bingham ◽  
Smita Kotnis ◽  
Barbara McHendry-Rinde ◽  
Ru Shen ◽  
Andrew Wood ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Cerebellar Granule Neurons ◽  
Granule Neurons ◽  
Cerebellar Granule ◽  
Laser Scanning Cytometry

scholarly journals A suitable method for identifying cell aggregates in laser scanning cytometry listmode data for analyzing disaggregated cell suspensions obtained from human cancers

Cytometry ◽  
2004 ◽  
Vol 59B (1) ◽  
pp. 10-23 ◽  
Author(s):  
Stanley E. Shackney ◽  
Charles A. Smith ◽  
Agnese A. Pollice ◽  
Kathryn Brown ◽  
Deborah Kosiban
Keyword(s):  
Laser Scanning ◽  
Cell Suspensions ◽  
Suitable Method ◽  
Cell Aggregates ◽  
Human Cancers ◽  
Laser Scanning Cytometry

scholarly journals Analysis of Cell Cycle and Replication of Mouse Macrophages afterIn VivoandIn VitroCryptococcus neoformans Infection Using Laser Scanning Cytometry

2012 ◽  
Vol 80 (4) ◽  
pp. 1467-1478 ◽  
Author(s):  
Carolina Coelho ◽  
Lydia Tesfa ◽  
Jinghang Zhang ◽  
Johanna Rivera ◽  
Teresa Gonçalves ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Division ◽  
Cytotoxic Effect ◽  
Laser Scanning ◽  
Cell Cycle Progression ◽  
Cycle Progression ◽  
Content Type ◽  
Cycle Arrest ◽  
Laser Scanning Cytometry

ABSTRACTWe investigated the outcome of the interaction ofCryptococcus neoformanswith murine macrophages using laser scanning cytometry (LSC). Previous results in our lab had shown that phagocytosis ofC. neoformanspromoted cell cycle progression. LSC allowed us to simultaneously measure the phagocytic index, macrophage DNA content, and 5-ethynyl-2′-deoxyuridine (EdU) incorporation such that it was possible to study host cell division as a function of phagocytosis. LSC proved to be a robust, reliable, and high-throughput method for quantifying phagocytosis. Phagocytosis ofC. neoformanspromoted cell cycle progression, but infected macrophages were significantly less likely to complete mitosis. Hence, we report a new cytotoxic effect associated with intracellularC. neoformansresidence that manifested itself in impaired cell cycle completion as a consequence of a block in the G2/M stage of the mitotic cell cycle. Cell cycle arrest was not due to increased cell membrane permeability or DNA damage. We investigated alveolar macrophage replicationin vivoand demonstrated that these cells are capable of low levels of cell division in the presence or absence ofC. neoformansinfection. In summary, we simultaneously studied phagocytosis, the cell cycle state of the host cell and pathogen-mediated cytotoxicity, and our results demonstrate a new cytotoxic effect ofC. neoformansinfection on murine macrophages: fungus-induced cell cycle arrest. Finally, we provide evidence for alveolar macrophage proliferationin vivo.

scholarly journals Laser scanning cytometry (LCS) allows detailed analysis of the cell cycle in PI stained human fibroblasts (TIG-7)

1997 ◽  
Vol 30 (3) ◽  
pp. 139-147 ◽  
Author(s):  
M. Kawasaki ◽  
K. Sasaki ◽  
T. Satoh ◽  
A. Kurose ◽  
T. Kamada ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Detailed Analysis ◽  
Laser Scanning ◽  
Human Fibroblasts ◽  
Laser Scanning Cytometry
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