scholarly journals Super-Resolution Microscopy Gives New Insights into Nuclear Pore Complex Organization

BioTechniques ◽  
2014 ◽  
Vol 57 (4) ◽  
Keyword(s):  
Nuclear Pore Complex ◽  
Super Resolution ◽  
Nuclear Pore ◽  
Complex Organization ◽  
Super Resolution Microscopy

scholarly journals Nuclear pore complex plasticity during developmental process as revealed by super-resolution microscopy

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Julien Sellés ◽  
May Penrad-Mobayed ◽  
Cyndélia Guillaume ◽  
Alica Fuger ◽  
Loïc Auvray ◽  
...  
Keyword(s):  
Nuclear Pore Complex ◽  
Developmental Process ◽  
Super Resolution ◽  
Nuclear Pore ◽  
Super Resolution Microscopy

scholarly journals Super-resolution mapping of scaffold nucleoporins in the nuclear pore complex

2017 ◽  
Vol 130 (7) ◽  
pp. 1299-1306 ◽  
Author(s):  
Jiong Ma ◽  
Joseph M. Kelich ◽  
Samuel L. Junod ◽  
Weidong Yang
Keyword(s):  
Nuclear Pore Complex ◽  
Super Resolution ◽  
Nuclear Pore ◽  
Resolution Mapping ◽  
Super Resolution Mapping

Nuclear Pore Scaffold Structure Analyzed by Super-Resolution Microscopy and Particle Averaging

Science ◽  
2013 ◽  
Vol 341 (6146) ◽  
pp. 655-658 ◽  
Author(s):  
Anna Szymborska ◽  
Alex de Marco ◽  
Nathalie Daigle ◽  
Volker C. Cordes ◽  
John A. G. Briggs ◽  
...  
Keyword(s):  
Protein Complexes ◽  
Super Resolution ◽  
Molecular Organization ◽  
Nuclear Pore ◽  
Protein Assemblies ◽  
Large Protein ◽  
Whole Cells ◽  
Molecular Machinery ◽  
Scaffold Structure ◽  
Super Resolution Microscopy

Much of life’s essential molecular machinery consists of large protein assemblies that currently pose challenges for structure determination. A prominent example is the nuclear pore complex (NPC), for which the organization of its individual components remains unknown. By combining stochastic super-resolution microscopy, to directly resolve the ringlike structure of the NPC, with single particle averaging, to use information from thousands of pores, we determined the average positions of fluorescent molecular labels in the NPC with a precision well below 1 nanometer. Applying this approach systematically to the largest building block of the NPC, the Nup107-160 subcomplex, we assessed the structure of the NPC scaffold. Thus, light microscopy can be used to study the molecular organization of large protein complexes in situ in whole cells.

scholarly journals Super-Resolution by Feedback Imaging: Mechanisms of Translocation through the Nuclear Pore Complex

2013 ◽  
Vol 104 (2) ◽  
pp. 120a
Author(s):  
Francesco Cardarelli ◽  
Luca Lanzano ◽  
Enrico Gratton
Keyword(s):  
Nuclear Pore Complex ◽  
Super Resolution ◽  
Nuclear Pore ◽  
Imaging Mechanisms

scholarly journals Correlative super-resolution fluorescence and electron microscopy of the nuclear pore complex with molecular resolution

2014 ◽  
Vol 127 (20) ◽  
pp. 4351-4355 ◽  
Author(s):  
Anna Löschberger ◽  
Christian Franke ◽  
Georg Krohne ◽  
Sebastian van de Linde ◽  
Markus Sauer
Keyword(s):  
Electron Microscopy ◽  
Nuclear Pore Complex ◽  
Super Resolution ◽  
Nuclear Pore ◽  
Fluorescence And Electron Microscopy

Dynamics of nuclear pore complex organization through the cell cycle

2004 ◽  
Vol 16 (3) ◽  
pp. 314-321 ◽  
Author(s):  
Gwénaël Rabut ◽  
Péter Lénárt ◽  
Jan Ellenberg
Keyword(s):  
Cell Cycle ◽  
Nuclear Pore Complex ◽  
Nuclear Pore ◽  
Complex Organization
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