scholarly journals Detection of antigen interactions ex vivo by proximity ligation assay: endogenous dopamine D2-adenosine A2A receptor complexes in the striatum

BioTechniques ◽  
2011 ◽  
Author(s):  
Pierre Trifilieff ◽  
Marie-Laure Rives ◽  
Eneko Urizar ◽  
Rebecca Piskorowski ◽  
Harshad Vishwasrao ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Adenosine A2a Receptor ◽  
Proximity Ligation Assay ◽  
Proximity Ligation ◽  
Receptor Complexes ◽  
Dopamine D2 ◽  
Endogenous Dopamine ◽  
A2a Receptor ◽  
Adenosine A2a

scholarly journals Radiation Dosimetry of a Novel Adenosine A2A Receptor Radioligand [11C]Preladenant Based on PET/CT Imaging and Ex Vivo Biodistribution in Rats

2016 ◽  
Vol 19 (2) ◽  
pp. 289-297 ◽  
Author(s):  
Xiaoyun Zhou ◽  
Philip H. Elsinga ◽  
Shivashankar Khanapur ◽  
Rudi A. J. O. Dierckx ◽  
Erik F. J. de Vries ◽  
...  
Keyword(s):  
Radiation Dosimetry ◽  
Ex Vivo ◽  
Ct Imaging ◽  
Adenosine A2a Receptor ◽  
Pet Ct ◽  
A2a Receptor ◽  
Adenosine A2a ◽  
Ex Vivo Biodistribution

scholarly journals Ex Vivo Perfusion With Adenosine A2A Receptor Agonist Enhances Rehabilitation of Murine Donor Lungs After Circulatory Death

2015 ◽  
Vol 99 (12) ◽  
pp. 2494-2503 ◽  
Author(s):  
Matthew L. Stone ◽  
Ashish K. Sharma ◽  
Valeria R. Mas ◽  
Ricardo C. Gehrau ◽  
Daniel P. Mulloy ◽  
...  
Keyword(s):  
Receptor Agonist ◽  
Ex Vivo ◽  
Adenosine A2a Receptor ◽  
Ex Vivo Perfusion ◽  
A2a Receptor ◽  
Adenosine A2a ◽  
Circulatory Death

scholarly journals Dopamine D2 receptor-mediated modulation of adenosine A2A receptor agonist binding within the A2AR/D2R oligomer framework

2013 ◽  
Vol 63 (1) ◽  
pp. 42-46 ◽  
Author(s):  
Víctor Fernández-Dueñas ◽  
Maricel Gómez-Soler ◽  
Xavier Morató ◽  
Fabiana Núñez ◽  
Arijit Das ◽  
...  
Keyword(s):  
Receptor Agonist ◽  
Dopamine D2 Receptor ◽  
D2 Receptor ◽  
Adenosine A2a Receptor ◽  
Agonist Binding ◽  
Dopamine D2 ◽  
A2a Receptor ◽  
Adenosine A2a

scholarly journals Ex vivo lung perfusion with adenosine A2A receptor agonist allows prolonged cold preservation of lungs donated after cardiac death

2016 ◽  
Vol 151 (2) ◽  
pp. 538-546 ◽  
Author(s):  
Cynthia E. Wagner ◽  
Nicolas H. Pope ◽  
Eric J. Charles ◽  
Mary E. Huerter ◽  
Ashish K. Sharma ◽  
...  
Keyword(s):  
Cardiac Death ◽  
Receptor Agonist ◽  
Ex Vivo ◽  
Lung Perfusion ◽  
Adenosine A2a Receptor ◽  
Ex Vivo Lung Perfusion ◽  
Cold Preservation ◽  
A2a Receptor ◽  
Adenosine A2a

scholarly journals Evaluation of the Effect of GABAB Agonists on the Vagal Nodose C-Fibers in the Esophagus

2013 ◽  
pp. 285-295 ◽  
Author(s):  
M. BROZMANOVÁ ◽  
L. MAZÚROVÁ ◽  
M. TATÁR ◽  
M. KOLLÁRIK
Keyword(s):  
Mechanical Response ◽  
Gabab Receptor ◽  
Ex Vivo ◽  
Adenosine A2a Receptor ◽  
H1 Receptor ◽  
Esophageal Diseases ◽  
Beneficial Effects ◽  
C Fibers ◽  
A2a Receptor ◽  
Adenosine A2a

Clinical studies showed that GABAB receptor agonists improve symptoms in patients with gastroesophageal reflux disease. One proposed mechanism of this effect is direct inhibition of the gastroesophageal vagal tension mechanosensors by GABAB agonists leading to reduction of reflux. In addition to tension mechanosensors, the vagal nodose ganglion supplies the esophagus with nociceptive C-fibers that likely contribute to impairment of esophageal reflex regulation in diseases. We hypothesized that GABAB agonists inhibit mechanically-induced activation of vagal esophageal nodose C-fibers in baseline and/or in sensitized state induced by inflammatory mediators. Ex vivo extracellular recordings were made from the esophageal nodose C-fibers in the isolated vagally-innervated guinea pig esophagus. We found that the selective GABAB agonist baclofen (100-300 µM) did not inhibit activation of esophageal nodose C-fibers evoked by esophageal distention (10-60 mmHg). The mechanical response of esophageal nodose C-fibers can be sensitized by different pathways including the stimulation of the histamine H1 receptor and the stimulation the adenosine A2A receptor. Baclofen failed to inhibit mechanical sensitization of esophageal nodose C-fibers induced by histamine (100 µM) or the selective adenosine A2A receptor agonist CGS21680 (3 nM). Our data suggest that the direct mechanical inhibition of nodose C-fibers in the esophagus is unlikely to contribute to beneficial effects of GABAB agonists in patients with esophageal diseases.

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