A bacterial two-hybrid system that utilizes Gateway cloning for rapid screening of protein-protein interactions

S.L. Rajasekhar Karna; Xhavit Zogaj; Jeffrey R. Barker; Janakiram Seshu; Simon L. Dove; Karl E. Klose

doi:10.2144/000113539

A bacterial two-hybrid system that utilizes Gateway cloning for rapid screening of protein-protein interactions

BioTechniques ◽

10.2144/000113539 ◽

2010 ◽

Vol 49 (5) ◽

pp. 831-833 ◽

Cited By ~ 14

Author(s):

S.L. Rajasekhar Karna ◽

Xhavit Zogaj ◽

Jeffrey R. Barker ◽

Janakiram Seshu ◽

Simon L. Dove ◽

...

Keyword(s):

Hybrid System ◽

Protein Interactions ◽

Rapid Screening ◽

Protein Protein Interactions ◽

Gateway Cloning ◽

Two Hybrid

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Faculty Opinions recommendation of A tethered catalysis, two-hybrid system to identify protein-protein interactions requiring post-translational modifications.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1020074.241459 ◽

2004 ◽

Author(s):

Igor Stagljar

Keyword(s):

Hybrid System ◽

Protein Interactions ◽

Protein Protein Interactions ◽

Post Translational Modifications ◽

Two Hybrid

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One- Plus Two-Hybrid System for the Efficient Selection of Missense Mutant Alleles Defective in Protein–Protein Interactions

Methods in Molecular Biology - Two Hybrid Technologies ◽

10.1007/978-1-61779-455-1_12 ◽

2011 ◽

pp. 209-223 ◽

Cited By ~ 1

Author(s):

Ji Young Kim ◽

Ok Gu Park ◽

Young Chul Lee

Keyword(s):

Hybrid System ◽

Protein Interactions ◽

Protein Protein Interactions ◽

Missense Mutant ◽

Efficient Selection ◽

Two Hybrid ◽

Selection Of

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The Yeast Two-Hybrid System: A Tool for Mapping Protein–Protein Interactions

Cold Spring Harbor Protocols ◽

10.1101/pdb.top083345 ◽

2015 ◽

Vol 2015 (5) ◽

pp. pdb.top083345 ◽

Cited By ~ 17

Author(s):

Jitender Mehla ◽

J. Harry Caufield ◽

Peter Uetz

Keyword(s):

Hybrid System ◽

Protein Interactions ◽

Protein Protein Interactions ◽

Yeast Two Hybrid ◽

System A ◽

Yeast Two Hybrid System ◽

Two Hybrid

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Yeast Two-Hybrid System for Studying Protein-Protein Interactions--Stage 2: Transforming and Characterizing the Library

Cold Spring Harbor Protocols ◽

10.1101/pdb.prot5430 ◽

2010 ◽

Vol 2010 (5) ◽

pp. pdb.prot5430-pdb.prot5430

Author(s):

I. Serebriiskii

Keyword(s):

Hybrid System ◽

Protein Interactions ◽

Protein Protein Interactions ◽

Yeast Two Hybrid ◽

Yeast Two Hybrid System ◽

Two Hybrid

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[16] Analyzing protein-protein interactions using two-hybrid system

Oncogene techniques - Methods in Enzymology ◽

10.1016/0076-6879(95)54018-0 ◽

1995 ◽

pp. 241-263 ◽

Cited By ~ 240

Author(s):

Paul L. Bartel ◽

Stanley Fields

Keyword(s):

Hybrid System ◽

Protein Interactions ◽

Protein Protein Interactions ◽

Two Hybrid

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Studying Protein-Protein Interactions Using a Bacterial Two-Hybrid System

E. coli Gene Expression Protocols ◽

10.1385/1-59259-301-1:251 ◽

2003 ◽

pp. 251-266

Author(s):

Simon L. Dove

Keyword(s):

Hybrid System ◽

Protein Interactions ◽

Protein Protein Interactions ◽

Two Hybrid

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The Bacterial Two-Hybrid System as a Reporter System for Analyzing Protein-Protein Interactions

Cold Spring Harbor Protocols ◽

10.1101/pdb.prot4672 ◽

2007 ◽

Vol 2007 (3) ◽

pp. pdb.prot4672 ◽

Cited By ~ 2

Author(s):

Astrid V. Giesecke ◽

J. Keith Joung

Keyword(s):

Hybrid System ◽

Protein Interactions ◽

Protein Protein Interactions ◽

Reporter System ◽

Two Hybrid

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Detection of Protein-Protein Interactions Using a Green Fluorescent Protein-Based Mammalian Two-Hybrid System

BioTechniques ◽

10.2144/00291bm01 ◽

2000 ◽

Vol 29 (1) ◽

pp. 22-26 ◽

Cited By ~ 2

Author(s):

M. Fotin-Mleczek ◽

M. Rottmann ◽

G. Rehg ◽

S. Rupp ◽

F.-J. Johannes

Keyword(s):

Green Fluorescent Protein ◽

Hybrid System ◽

Protein Interactions ◽

Fluorescent Protein ◽

Protein Protein Interactions ◽

Green Fluorescent ◽

Two Hybrid

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High Throughput Mapping of Protein-Protein Interactions: Automation of the Yeast Two-Hybrid System

JALA Journal of the Association for Laboratory Automation ◽

10.1016/s1535-5535-04-00192-3 ◽

2002 ◽

Vol 7 (3) ◽

pp. 38-40 ◽

Cited By ~ 1

Author(s):

Steve A. Richmond ◽

Kelly Gregan ◽

Julian F. Burke

Keyword(s):

Hybrid System ◽

High Throughput ◽

Protein Interactions ◽

Protein Protein Interactions ◽

Yeast Two Hybrid ◽

Yeast Two Hybrid System ◽

Two Hybrid

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The reverse two-hybrid system: a genetic scheme for selection against specific protein/protein interactions

Nucleic Acids Research ◽

10.1093/nar/24.17.3341 ◽

1996 ◽

Vol 24 (17) ◽

pp. 3341-3347 ◽

Cited By ~ 46

Author(s):

C. Leanna

Keyword(s):

Hybrid System ◽

Protein Interactions ◽

Specific Protein ◽

Protein Protein Interactions ◽

System A ◽

Two Hybrid

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