scholarly journals Ambient temperature stabilization of purified RNA in GenTegra™ for use in Affymetrix Human Exon 1.0 ST arrays

BioTechniques ◽  
2010 ◽  
Vol 48 (6) ◽  
pp. 468-469 ◽  
Author(s):  
M. G. Barnes ◽  
M. Tsoras ◽  
S. D. Thompson ◽  
H. Martinez ◽  
B. Iverson ◽  
...  
Keyword(s):  
Ambient Temperature ◽  
Temperature Stabilization ◽  
Human Exon

Ambient temperature stabilization of crystalline zirconia thin films deposited by direct current magnetron sputtering

2009 ◽  
Vol 517 (5) ◽  
pp. 1587-1591 ◽  
Author(s):  
D. Pamu ◽  
K. Sudheendran ◽  
M. Ghanashyam Krishna ◽  
K.C. James Raju ◽  
Anil K. Bhatnagar
Keyword(s):  
Thin Films ◽  
Magnetron Sputtering ◽  
Ambient Temperature ◽  
Direct Current ◽  
Temperature Stabilization ◽  
Direct Current Magnetron Sputtering

scholarly journals Improving the Efficiency of Measurements of Combustible Gases Concentration by a Thermocatalytic Sensor

2020 ◽  
Vol 24 (1) ◽  
pp. 189-205
Author(s):  
O. G. Bondar ◽  
E. O. Brezhneva
Keyword(s):  
Ambient Temperature ◽  
Mathematical Description ◽  
Temperature Fluctuations ◽  
Conversion Function ◽  
Temperature Stabilization ◽  
Temperature Influence ◽  
Gas Concentration ◽  
Conversion Characteristic ◽  
Combustible Gases ◽  
Tuning Out

Thermocatalytic sensors are widely used in gas analysis systems and have high reliability and low cost. However, errors in measuring the concentration of combustible gases related to the non-linearity of the conversion characteristic and the influence of ambient temperature fluctuations significantly limit the scope of their application.Purpose of reseach. Development of a method for measuring gas concentration by thermocatalytic sensors, which allows reducing measurement errors by tuning out due to ambient temperature influence and linearization of the conversion characteristic.Problems. They are as follows: to develop a method for temperature stabilization of a thermocatalytic sensor; to make a structural and functional scheme for the sensor activation; to obtain a mathematical description of the method and substantitation for tuning out as a result of temperature influence; to experimentally confirm the possibility of linearization of the sensor conversion function in the thermal stabilization mode.Methods. The mathematical description of the method applies the theory of heat transfer and the theory of electrical circuits with discrete signals. When analyzing existing solutions and synthesizing the device, methods for calculating circuits with nonlinear elements and the theory of measurement systems have been used. The real conversion function has been obtained through an experimental method.Results. A method for measuring gas concentration by a thermocatalytic sensor with the use of a microcontroller and PWM has been developed. It allows reducing errors due to tuning out as a result of ambient temperature influence. A mathematical description of the method has been given. An experiment has been performed. It demonstrates the effectiveness of using temperature stabilization to linearize the conversion characteristic.Conclusion. The paper proposes a method for temperature stabilization of thermocatalytic gas sensors. The method makes it possible to increase the accuracy of measurements by tuning out due to the influence of temperature fluctuations and linearization of the conversion function. The possibility of linearization of the sensor function has been experimentally confirmed. It characterizes the dependence of the output signal on the concentration of combustible gas. Using this method allows you to reduce the cost of the sensor, improve the quality factors of the sensor, such as the reliability and stability of parameters.

SEM of non-coated hepatocellular cytoskeleton of perfused livers

1984 ◽  
Vol 42 ◽  
pp. 306-307
Author(s):  
S.W. French ◽  
N.C. Benson ◽  
C. Davis-Scibienski
Keyword(s):  
Ambient Temperature ◽  
Critical Point ◽  
Protease Inhibitors ◽  
Metal Deposition ◽  
Heat Damage ◽  
Detergent Extraction ◽  
Cytoskeletal Elements ◽  
Triton X ◽  
Excised Tissue

Previous SEM studies of liver cytoskeletal elements have encountered technical difficulties such as variable metal coating and heat damage which occurs during metal deposition. The majority of studies involving evaluation of the cell cytoskeleton have been limited to cells which could be isolated, maintained in culture as a monolayer and thus easily extracted. Detergent extraction of excised tissue by immersion has often been unsatisfactory beyond the depth of several cells. These disadvantages have been avoided in the present study. Whole C3H mouse livers were perfused in situ with 0.5% Triton X-100 in a modified Jahn's buffer including protease inhibitors. Perfusion was continued for 1 to 2 hours at ambient temperature. The liver was then perfused with a 2% buffered gluteraldehyde solution. Liver samples including spontaneous tumors were then maintained in buffered gluteraldehyde for 2 hours. Samples were processed for SEM and TEM using the modified thicarbohydrazide procedure of Malich and Wilson, cryofractured, and critical point dried (CPD). Some samples were mechanically fractured after CPD.

Formation of Dislocation Channels in Neutron Irradiated Molybdenum

1972 ◽  
Vol 30 ◽  
pp. 672-673
Author(s):  
S. Mahajan
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ambient Temperature ◽  
Room Temperature ◽  
Channel Formation ◽  
Early Stages ◽  
Transmission Electron ◽  
Three Stages ◽  
Two Stages ◽  
Polycrystalline Molybdenum

The evolution of dislocation channels in irradiated metals during deformation can be envisaged to occur in three stages: (i) formation of embryonic cluster free regions, (ii) growth of these regions into microscopically observable channels and (iii) termination of their growth due to the accumulation of dislocation damage. The first two stages are particularly intriguing, and we have attempted to follow the early stages of channel formation in polycrystalline molybdenum, irradiated to 5×1019 n. cm−2 (E > 1 Mev) at the reactor ambient temperature (∼ 60°C), using transmission electron microscopy. The irradiated samples were strained, at room temperature, up to the macroscopic yield point.Figure 1 illustrates the early stages of channel formation. The observations suggest that the cluster free regions, such as A, B and C, form in isolated packets, which could subsequently link-up to evolve a channel.

Crystallographic Characterization of Dislocation Loops in Irradiated Tungsten

1968 ◽  
Vol 26 ◽  
pp. 290-291
Author(s):  
Robert C. Rau
Keyword(s):  
Electron Microscope ◽  
Ambient Temperature ◽  
Dislocation Loops ◽  
Polycrystalline Specimen ◽  
Post Irradiation ◽  
Neutron Fluences

Previous work has shown that post-irradiation annealing, at temperatures near 1100°C, produces resolvable dislocation loops in tungsten irradiated to fast (E > 1 MeV) neutron fluences of about 4 x 1019 n/cm2 or greater. To crystallographically characterize these loops, tilting experiments were carried out in the electron microscope on a polycrystalline specimen which had been irradiated to 1.5 × 1021 n/cm2 at reactor ambient temperature (∼ 70°C), and subseouently annealed for 315 hours at 1100°C. This treatment produced large loops averaging 1000 Å in diameter, as shown in the micrographs of Fig. 1. The orientation of this grain was near (001), and tilting was carried out about axes near [100], [10] and [110].

Voids in Quenched Nickel

1968 ◽  
Vol 26 ◽  
pp. 266-267
Author(s):  
J. J. Laidler
Keyword(s):  
Electron Beam ◽  
Ambient Temperature ◽  
Cooling Rate ◽  
Three Dimensional ◽  
Cooling Curve ◽  
Polycrystalline Nickel ◽  
Quenching Temperature ◽  
Long Tail ◽  
Diffusion Pump

The presence of three-dimensional voids in quenched metals has long been suspected, and voids have indeed been observed directly in a number of metals. These include aluminum, platinum, and copper, silver and gold. Attempts at the production of observable quenched-in defects in nickel have been generally unsuccessful, so the present work was initiated in order to establish the conditions under which such defects may be formed.Electron beam zone-melted polycrystalline nickel foils, 99.997% pure, were quenched from 1420°C in an evacuated chamber into a bath containing a silicone diffusion pump fluid . The pressure in the chamber at the quenching temperature was less than 10-5 Torr . With an oil quench such as this, the cooling rate is approximately 5,000°C/second above 400°C; below 400°C, the cooling curve has a long tail. Therefore, the quenched specimens are aged in place for several seconds at a temperature which continuously approaches the ambient temperature of the system.

STUDY ON DEVELOPMENT OF PASTA PRODUCT WITH INCORPORATION OF OCIMUM SANCTUM (BASIL) TO ENHANCE THE SENSORY AND NUTRITIONAL QUALITY

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
PIYUSH MISHRA ◽  
DEVENDRA KUMAR BHATT
Keyword(s):  
Ambient Temperature ◽  
Shelf Life ◽  
Nutritional Quality ◽  
Leaf Extract ◽  
Storage Stability ◽  
Natural Antioxidants ◽  
Sensory Properties ◽  
Ocimum Sanctum ◽  
Leaf Powder

Pasta was prepared by incorporation of Ocimum sanctum (Basil) for better textural and sensory properties. The pasta was incorporated with the leaf extract of Ocimum sanctum at different concentrations of control, 5, 10, and 15.The natural antioxidants present in the O. sanctum leaf powder that was incorporated in the fruit leather showed extended shelf-life over three months when compared with control, without any added preservative at ambient temperature. Also the nutritional stability of the product was studied under two flexible packages of polypropylene and polyester out of that the products packed in polypropylene showed better storage stability .

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