scholarly journals Cationic lipid-mediated intracellular delivery of antibodies into live cells

BioTechniques ◽  
2008 ◽  
Vol 44 (7S) ◽  
pp. vii-xi ◽  
Author(s):  
Claire O. Weill ◽  
Stéphanie Biri ◽  
Patrick Erbacher
Keyword(s):  
Cationic Lipid ◽  
Intracellular Delivery ◽  
Live Cells

Cationic Lipid‐based Intracellular Delivery of Bacterial Effectors for Rewiring Malignant Cell Signaling

2020 ◽  
Vol 59 (41) ◽  
pp. 18087-18094 ◽  
Author(s):  
Shaojun Yang ◽  
Qiao Tang ◽  
Long Chen ◽  
Jin Chang ◽  
Tian Jiang ◽  
...  
Keyword(s):  
Cell Signaling ◽  
Cationic Lipid ◽  
Intracellular Delivery ◽  
Malignant Cell ◽  
Bacterial Effectors

Intracellular Delivery of Nanobodies for Imaging of Target Proteins in Live Cells

2016 ◽  
Vol 34 (1) ◽  
pp. 161-174 ◽  
Author(s):  
Ruth Röder ◽  
Jonas Helma ◽  
Tobias Preiß ◽  
Joachim O. Rädler ◽  
Heinrich Leonhardt ◽  
...  
Keyword(s):  
Intracellular Delivery ◽  
Live Cells ◽  
Target Proteins

scholarly journals A Chemical-genetics and Nanoparticle Enabled Approach for in vivo Protein Kinase Analysis

2020 ◽  
Author(s):  
Fengqian Chen ◽  
Qi Liu ◽  
Terrell Hilliard ◽  
Tingzeng Wang ◽  
Hongjun Liang ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinases ◽  
Intracellular Delivery ◽  
Binding Pocket ◽  
Live Cells ◽  
Genetic Method ◽  
Chemical Genetic ◽  
Methionine Residue ◽  
Two Factors

AbstractThe human kinome contains >500 protein kinases, and regulates up to 30% of the proteome. Kinase study is currently hindered by a lack of in vivo analysis approaches due to two factors: our inability to distinguish the kinase reaction of interest from those of other kinases in live cells and the cell impermeability of the ATP analogs. Herein, we tackled this issue by combining the widely used chemical genetic method developed by Dr. Kevan Shokat and colleagues with nanoparticle-mediated intracellular delivery of the ATP analog. The critical AKT1 protein kinase, which has been successfully studied with the method, was used as our initial prototype. Briefly, enlargement of the ATP binding pocket, by mutating the gate-keeper Methionine residue to a Glycine, prompted the mutant AKT1 to preferentially use the bulky ATP analog N6-Benzyl-ATP-γ-S (A*TPγS) and, thus, differentiating AKT1-catalyzed and other phosphorylation events. The lipid/calcium/phosphate (LCP) nanoparticle was used for efficient intracellular delivery of A*TPγS, overcoming the cell impermeability issue. The mutant, but not wild-type, AKT1 used the delivered A*TPγS for autophosphorylation and phosphorylating its substrates in live cells. Thus, an in vivo protein kinase analysis method has been developed. The strategy should be widely applicable to other protein kinases.

Intracellular delivery of the PTEN protein using cationic lipidoids for cancer therapy

2016 ◽  
Vol 4 (12) ◽  
pp. 1773-1780 ◽  
Author(s):  
Sarah A. Altınoğlu ◽  
Ming Wang ◽  
Kathleen Q. Li ◽  
Yuyang Li ◽  
Qiaobing Xu
Keyword(s):  
Cancer Therapy ◽  
Tumor Suppressor ◽  
Cancer Cells ◽  
Treatment Approach ◽  
Cationic Lipid ◽  
Intracellular Delivery ◽  
Tumor Suppressor Protein ◽  
Pten Protein ◽  
Direct Treatment

The PTEN tumor suppressor protein encapsulated in cationic lipid-like nanoparticles as a direct treatment approach for PTEN-deficient cancer cells.

A new technique for reversible permeabilization of live cells for intracellular delivery of quantum dots

2013 ◽  
Vol 24 (20) ◽  
pp. 205101 ◽  
Author(s):  
Krishnakiran Medepalli ◽  
Bruce W Alphenaar ◽  
Robert S Keynton ◽  
Palaniappan Sethu
Keyword(s):  
Quantum Dots ◽  
Intracellular Delivery ◽  
New Technique ◽  
Live Cells ◽  
Reversible Permeabilization ◽  
A New Technique

Cationic lipid saturation influences intracellular delivery of encapsulated nucleic acids

2005 ◽  
Vol 107 (2) ◽  
pp. 276-287 ◽  
Author(s):  
James Heyes ◽  
Lorne Palmer ◽  
Kaz Bremner ◽  
Ian MacLachlan
Keyword(s):  
Nucleic Acids ◽  
Cationic Lipid ◽  
Intracellular Delivery

Fully synthetic polymer vesicles for intracellular delivery of antibodies in live cells

2012 ◽  
Vol 27 (1) ◽  
pp. 98-108 ◽  
Author(s):  
Irene Canton ◽  
Marzia Massignani ◽  
Nisa Patikarnmonthon ◽  
Luca Chierico ◽  
James Robertson ◽  
...  
Keyword(s):  
Intracellular Delivery ◽  
Synthetic Polymer ◽  
Live Cells ◽  
Polymer Vesicles

Cationic Lipid‐based Intracellular Delivery of Bacterial Effectors for Rewiring Malignant Cell Signaling

2020 ◽  
Vol 132 (41) ◽  
pp. 18243-18250
Author(s):  
Shaojun Yang ◽  
Qiao Tang ◽  
Long Chen ◽  
Jin Chang ◽  
Tian Jiang ◽  
...  
Keyword(s):  
Cell Signaling ◽  
Cationic Lipid ◽  
Intracellular Delivery ◽  
Malignant Cell ◽  
Bacterial Effectors

scholarly journals Guiding protein delivery into live cells using DNA-programmed membrane fusion

2018 ◽  
Vol 9 (27) ◽  
pp. 5967-5975 ◽  
Author(s):  
Lele Sun ◽  
Yanjing Gao ◽  
Yaoguang Wang ◽  
Qin Wei ◽  
Jiye Shi ◽  
...  
Keyword(s):  
Membrane Fusion ◽  
Protein Delivery ◽  
Intracellular Delivery ◽  
Live Cells ◽  
Fusion Strategy

A DNA-programmed membrane fusion strategy for directing intracellular delivery of proteins into live cells.

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