scholarly journals Assays for the rapid and specific identification of North AmericanYersinia pestisand the common laboratory strain CO92

BioTechniques ◽  
2008 ◽  
Vol 44 (2) ◽  
pp. 201-207 ◽  
Author(s):  
Amy J. Vogler ◽  
Elizabeth M. Driebe ◽  
Judy Lee ◽  
Raymond K. Auerbach ◽  
Christopher J. Allender ◽  
...  
Keyword(s):  
Laboratory Strain ◽  
Specific Identification ◽  
The Common ◽  
Common Laboratory

scholarly journals Pigeon-Associated Strains of Salmonella enterica Serovar Typhimurium Phage Type DT2 Have Genomic Rearrangements at rRNA Operons

2004 ◽  
Vol 72 (12) ◽  
pp. 7338-7341 ◽  
Author(s):  
R. Allen Helm ◽  
Steffen Porwollik ◽  
April E. Stanley ◽  
Stanley Maloy ◽  
Michael McClelland ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Phage Type ◽  
Laboratory Strain ◽  
Genomic Rearrangements ◽  
Serovar Typhimurium ◽  
The Common ◽  
Genomic Anomalies ◽  
Common Laboratory

ABSTRACT Strains from a subgroup of Salmonella enterica serovar Typhimurium frequently associated with pigeon infections were tested for genomic anomalies and virulence in mice. Some strains have a genomic inversion between rrn operons. Two prophages found in the common laboratory strain LT2 were absent. Pigeon-associated strains are still virulent in mice.

scholarly journals In Vitro Susceptibility of Isolates of Francisella tularensis Types A and B from North America

2008 ◽  
Vol 52 (6) ◽  
pp. 2276-2278 ◽  
Author(s):  
Sandra K. Urich ◽  
Jeannine M. Petersen
Keyword(s):  
North America ◽  
Francisella Tularensis ◽  
Antimicrobial Agents ◽  
Susceptibility Testing ◽  
Laboratory Strain ◽  
In Vitro Susceptibility ◽  
Testing Method ◽  
The Common ◽  
Common Laboratory

ABSTRACT Due to concern that Francisella tularensis, the causative agent of tularemia, may be used as a bioterrorist weapon, the Clinical and Laboratory Standards Institute recently provided a susceptibility testing method with breakpoints. Here, 169 isolates (92 type A and 77 type B) from North America were tested against seven antimicrobial agents (streptomycin, gentamicin, tetracycline, doxycycline, ciprofloxacin, levofloxacin, and chloramphenicol) used for the treatment of tularemia. The MICs for all of the isolates fell within the susceptible range. In addition, all isolates had MICs for erythromycin of 0.5 to 4 μg/ml, in contrast to an MIC of >256 μg/ml for the common laboratory strain LVS (live vaccine strain).

scholarly journals The life history of Hepatozoon leptodactyli (Lesage, 1908) Pessoa, 1970: a parasite of the common laboratory animal: the frog of the genus Leptodactylus

1973 ◽  
Vol 71 (1-2) ◽  
pp. 1-8 ◽  
Author(s):  
Sylvio Celso Goncalves da Costa ◽  
Samuel B. Pessoa ◽  
Neize de Moura Pereira ◽  
Tania Colombo
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Life History ◽  
Laboratory Animal ◽  
Peripheral Circulation ◽  
Experimental Conditions ◽  
History Of ◽  
The Central Nervous System ◽  
The Common ◽  
Common Laboratory

The main object of the present paper is to furnish a brief account to the knowledgement of Protozoa parasitic in common Brazilian frog of the genus Leptodactylus for general students in Zoology and for investigators that use this frog as a laboratory animal. Hepatozoon leptodactyli (Haemogregarina leptodactyli) was found in two species of frogs - Leptodactylus ocellatus and L. pentadactylus - in which develop schizogony whereas sporogony occurs in the leech Haementeria lutzi as was obtainded in experimental conditions. Intracellular forms have been found in peripheral circulation, chiefly in erythrocytes, but we have found them in leukocytes too. Tissue stages were found in frog, liver, lungs, spleen, gut, brain and heart. The occurence of hemogregarine in the Central Nervous System was recorded by Costa & al,(13) and Ball (2). Some cytochemical methods were employed in attempt to differentiate gametocytes from trophozoites in the peripheral blood and to characterize the cystic membrane as well. The speorogonic cycle was developed in only one specie of leech. A brief description of the parasite is given.

scholarly journals Reclassification of Shigella species as later heterotypic synonyms of Escherichia coli in the Genome Taxonomy Database

2021 ◽  
Author(s):  
Donovan H Parks ◽  
Maria Chuvochina ◽  
Peter R Reeves ◽  
Scott A Beatson ◽  
Philip Hugenholtz
Keyword(s):  
Escherichia Coli ◽  
Type Strain ◽  
Shigella Flexneri ◽  
Laboratory Strain ◽  
Single Species ◽  
Species Definition ◽  
E Coli ◽  
Shigella Species ◽  
K 12 ◽  
Common Laboratory

Members of the genus Shigella have high genomic similarity to Escherichia coli and are often considered to be atypical members of this species. In an attempt to retain Shigella species as recognizable entities, they were reclassified as Escherichia species in the Genome Taxonomy Database (GTDB) using an operational average nucleotide identity (ANI)-based approach nucleated around type strains. This resulted in nearly 80% of E. coli genomes being reclassified to new species including the common laboratory strain E. coli K-12 (to 'E. flexneri') because it is more closely related to the type strain of Shigella flexneri than it is to the type strain of E. coli. Here we resolve this conundrum by treating Shigella species as later heterotypic synonyms of E. coli, present evidence supporting this reclassification, and show that assigning E. coli/Shigella strains to a single species is congruent with the GTDB-adopted genomic species definition.

Acute Liver Failure

2018 ◽  
Vol 39 (05) ◽  
pp. 513-522 ◽  
Author(s):  
Priyanka Rajaram ◽  
Ram Subramanian
Keyword(s):  
Liver Failure ◽  
Acute Liver Failure ◽  
Multiorgan Failure ◽  
Drug Induced ◽  
Detailed Review ◽  
Drug Induced Liver Injury ◽  
The Common ◽  
Organ Dysfunctions ◽  
Common Laboratory

AbstractAcute liver failure (ALF) is a condition that can rapidly progress to multiorgan failure. This article focuses on the diagnosis and management of ALF. We provide a detailed review of the common etiologies of ALF, including acetaminophen overdose, viral hepatitis, drug-induced liver injury, Wilson's disease, and autoimmune hepatitis. The article then addresses how to recognize ALF and reviews the role of common laboratory and imaging tests in establishing this diagnosis. The remainder of the article details the management of hepatic and extrahepatic organ dysfunctions in ALF. The article concludes with a discussion regarding the prognostication of patients with ALF and the criteria for considering liver transplantation.

scholarly journals Differences in neutralizing antigenicity between laboratory and clinical isolates of HCoV-229E isolated in Japan in 2004–2008 depend on the S1 region sequence of the spike protein

2012 ◽  
Vol 93 (9) ◽  
pp. 1908-1917 ◽  
Author(s):  
Kazuya Shirato ◽  
Miyuki Kawase ◽  
Oshi Watanabe ◽  
Chika Hirokawa ◽  
Shutoku Matsuyama ◽  
...  
Keyword(s):  
Protein A ◽  
Laboratory Strain ◽  
Clinical Isolates ◽  
Pseudotyped Virus ◽  
S Protein ◽  
Human Sera ◽  
Antigenic Phenotype ◽  
Vesicular Stomatitis ◽  
The Common ◽  
The Difference

Human coronavirus (HCoV) is a causative agent of the common cold. Although HCoV is highly prevalent in the world, studies of the genomic and antigenic details of circulating HCoV strains have been limited. In this study, we compared four Japanese isolates with the standard HCoV-229E strain obtained from ATCC (ATCC-VR740) by focusing on the spike (S) protein, a major determinant of neutralizing antigen and pathogenicity. The isolates were found to have nucleotide deletions and a number of sequence differences in the S1 region of the S protein. We compared two of the Japanese isolates with the ATCC-VR740 strain by using virus-neutralizing assays consisting of infectious HCoV-229E particles and vesicular stomatitis virus (VSV)-pseudotyped virus carrying the HCoV-229E S protein. The two clinical isolates (Sendai-H/1121/04 and Niigata/01/08) did not react with antiserum to the ATCC-VR740 strain via the neutralizing test. We then constructed a pseudotype VSV-harboured chimeric S protein with the ATCC S1 and Sendai S2 regions or that with Sendai S1 and ATCC S2 regions and compared them by a neutralization test. The results revealed that the difference in the neutralizing antigenicity depends on the S1 region. This different antigenic phenotype was also confirmed by a neutralizing test with clinically isolated human sera. These results suggest that the HCoV-229E viruses prevalent in Japan are quite different from the laboratory strain ATCC-VR740 in terms of the S sequence and neutralization antigenicity, which is attributed to the difference in the S1 region.

A comparison of methods for the prediction of Cantonese noodle colour

1992 ◽  
Vol 72 (4) ◽  
pp. 1021-1029 ◽  
Author(s):  
J. E. Kruger ◽  
R. R. Matsuo ◽  
K. Preston
Keyword(s):  
Sodium Hydroxide ◽  
Polyphenol Oxidase ◽  
Quality Parameter ◽  
Laboratory Method ◽  
Alternative Methods ◽  
Comparison Of Methods ◽  
White Wheat ◽  
Flour Colour ◽  
The Common ◽  
Common Laboratory

The common laboratory method for preparing Cantonese raw noodle dough for colour evaluation requires 200–300 g flour, a Hobart mixer to mix the dough and an Ohtake laboratory noodle machine. Alternative methods requiring less flour or less costly apparatus were developed and compared. Although the brightness (L*) and yellowness (b*) were influenced by the method of noodle-dough preparation, variations between methods were consistent. Different formulations of Kansui had similar effects on noodle brightness and yellowness, with the exception of sodium hydroxide, which produced substantially increased yellowness. The rate of decrease in brightness with time was useful as a quality parameter in estimation of noodle colour and may be indicative, in some cases, of the presence of polyphenol oxidase enzymes. The ranges in brightness and yellowness of Cantonese noodle doughs are very similar for Canadian wheats, except for soft white wheat cultivars.Key words: Noodle, wheat, flour, colour, polyphenol oxidase

Sign in / Sign up

Export Citation Format

Share Document