scholarly journals Phase separation in the isolation and purification of membrane proteins

BioTechniques ◽  
2007 ◽  
Vol 43 (4) ◽  
pp. 427-440 ◽  
Author(s):  
Thomas Arnold ◽  
Dirk Linke
Keyword(s):  
Phase Separation ◽  
Membrane Proteins ◽  
Isolation And Purification

Triton X-114 phase separation in the isolation and purification of mouse liver microsomal membrane proteins

Methods ◽  
2011 ◽  
Vol 54 (4) ◽  
pp. 396-406 ◽  
Author(s):  
Rommel A. Mathias ◽  
Yuan-Shou Chen ◽  
Eugene A. Kapp ◽  
David W. Greening ◽  
Suresh Mathivanan ◽  
...  
Keyword(s):  
Phase Separation ◽  
Membrane Proteins ◽  
Mouse Liver ◽  
Microsomal Membrane ◽  
Isolation And Purification ◽  
Triton X

scholarly journals Glycosyl-phosphatidylinositol-anchored membrane proteins can be distinguished from transmembrane polypeptide-anchored proteins by differential solubilization and temperature-induced phase separation in Triton X-114

1991 ◽  
Vol 280 (3) ◽  
pp. 745-751 ◽  
Author(s):  
N M Hooper ◽  
A Bashir
Keyword(s):  
Phase Separation ◽  
Membrane Proteins ◽  
Aqueous Phase ◽  
Hydrophobic Membrane ◽  
Rich Phase ◽  
Glycosyl Phosphatidylinositol ◽  
Ionic Detergent ◽  
Membrane Spanning ◽  
Triton X ◽  
Insoluble Pellet

Treatment of kidney microvillar membranes with the non-ionic detergent Triton X-114 at 0 degrees C, followed by low-speed centrifugation, generated a detergent-insoluble pellet and a detergent-soluble supernatant. The supernatant was further fractionated by phase separation at 30 degrees C into a detergent-rich phase and a detergent-depleted or aqueous phase. Those ectoenzymes with a covalently attached glycosyl-phosphatidylinositol (G-PI) membrane anchor were recovered predominantly (greater than 73%) in the detergent-insoluble pellet. In contrast, those ectoenzymes anchored by a single membrane-spanning polypeptide were recovered predominantly (greater than 62%) in the detergent-rich phase. Removal of the hydrophobic membrane-anchoring domain from either class of ectoenzyme resulted in the proteins being recovered predominantly (greater than 70%) in the aqueous phase. This technique was also applied to other membrane types, including pig and human erythrocyte ghosts, where, in both cases, the G-PI-anchored acetylcholinesterase partitioned predominantly (greater than 69%) into the detergent-insoluble pellet. When the microvillar membranes were subjected only to differential solubilization with Triton X-114 at 0 degrees C, the G-PI-anchored ectoenzymes were recovered predominantly (greater than 63%) in the detergent-insoluble pellet, whereas the transmembrane-polypeptide-anchored ectoenzymes were recovered predominantly (greater than 95%) in the detergent-solubilized supernatant. Thus differential solubilization and temperature-induced phase separation in Triton X-114 distinguished between G-PI-anchored membrane proteins, transmembrane-polypeptide-anchored proteins and soluble, hydrophilic proteins. This technique may be more useful and reliable than susceptibility to release by phospholipases as a means of identifying a G-PI anchor on an unpurified membrane protein.

scholarly journals Partitioning the Proteome: Phase Separation for Targeted Analysis of Membrane Proteins in Human Post-Mortem Brain

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e39509 ◽  
Author(s):  
Jane A. English ◽  
Bruno Manadas ◽  
Caitriona Scaife ◽  
David R. Cotter ◽  
Michael J. Dunn
Keyword(s):  
Phase Separation ◽  
Membrane Proteins ◽  
Post Mortem ◽  
Targeted Analysis ◽  
Post Mortem Brain

Divide and conquer: How phase separation contributes to lateral transport and organization of membrane proteins and lipids

2020 ◽  
Vol 233 ◽  
pp. 104985
Author(s):  
Ethan J. Miller ◽  
Amanda M. Ratajczak ◽  
Autumn A. Anthony ◽  
Matthew Mottau ◽  
Xaymara I. Rivera Gonzalez ◽  
...  
Keyword(s):  
Phase Separation ◽  
Membrane Proteins ◽  
Divide And Conquer ◽  
Lateral Transport

scholarly journals Fractionation of membrane proteins by temperature-induced phase separation in Triton X-114. Application to subcellular fractions of the adrenal medulla

1986 ◽  
Vol 233 (2) ◽  
pp. 525-533 ◽  
Author(s):  
J G Pryde ◽  
J H Phillips
Keyword(s):  
Endoplasmic Reticulum ◽  
Phase Separation ◽  
Membrane Proteins ◽  
Low Temperature ◽  
Membrane Glycoproteins ◽  
Chromaffin Granule ◽  
Rich Phase ◽  
Granule Membrane ◽  
Separation Of Proteins ◽  
Triton X

After solubilization with the detergent Triton X-114, membrane proteins may be separated into three groups: if the membrane is sufficiently lipid-rich, one family of hydrophobic constituents separates spontaneously at low temperature; warming at 30 degrees C leads to separation of a detergent-rich phase and an aqueous phase. Using the chromaffin-granule membrane as a model, we found that many intrinsic membrane glycoproteins are found in the latter phase, probably maintained in solution by adherent detergent. They precipitate, however, when this is removed by dialysis, leaving in solution those truly hydrophilic proteins that were originally adhering to the membranes. We have used this method with mitochondria, and with Golgi- and rough-endoplasmic-reticulum-enriched microsomal fractions: it has proved to be a rapid and convenient method for effecting a partial separation of proteins from a variety of different membranes.

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