scholarly journals Magnetic resonance-based visualization of gene expression in mammalian cells using a bacterial polyphosphate kinase reporter gene

BioTechniques ◽  
2007 ◽  
Vol 42 (2) ◽  
pp. 209-215 ◽  
Author(s):  
Sewon Ki ◽  
Fuminori Sugihara ◽  
Koji Kasahara ◽  
Hidehito Tochio ◽  
Masahiro Shirakawa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Magnetic Resonance ◽  
Reporter Gene ◽  
Mammalian Cells ◽  
Polyphosphate Kinase ◽  
Expression In Mammalian Cells

scholarly journals Molecular Imaging with Genetically Programmed Nanoparticles

2021 ◽  
Author(s):  
Donna E. Goldhawk
Keyword(s):  
Gene Expression ◽  
Molecular Imaging ◽  
Magnetic Resonance ◽  
Reporter Gene ◽  
Protein Interactions ◽  
Mammalian Cells ◽  
Reporter Gene Expression ◽  
Cell Therapies ◽  
Membrane Compartment

Nanoparticle research has greatly benefitted medical imaging platforms by generating new signals, enhancing detection sensitivity, and expanding both clinical and preclinical applications. For magnetic resonance imaging, the fabrication of superparamagnetic iron oxide nanoparticles has provided a means of detecting cells and has paved the way for magnetic particle imaging. As the field of molecular imaging grows and enables the tracking of cells and their molecular activities so does the possibility of tracking genetically programmed biomarkers. This chapter discusses the advantages and challenges of gene-based contrast, using the bacterial magnetosome model to highlight the requirements of in vivo iron biomineralization and reporter gene expression for magnetic resonance signal detection. New information about magnetosome protein interactions in non-magnetic mammalian cells is considered in the light of design and application(s) of a rudimentary magnetosome-like nanoparticle for molecular imaging. Central to this is the hypothesis that a magnetosome root structure is defined by essential magnetosome genes, whose expression positions the biomineral in a given membrane compartment, in any cell type. The use of synthetic biology for programming multi-component structures not only broadens the scope of reporter gene expression for molecular MRI but also facilitates the tracking of cell therapies.

scholarly journals Integrated Genomic and Proteomic Analyses of Gene Expression in Mammalian Cells

2004 ◽  
Vol 3 (10) ◽  
pp. 960-969 ◽  
Author(s):  
Qiang Tian ◽  
Serguei B. Stepaniants ◽  
Mao Mao ◽  
Lee Weng ◽  
Megan C. Feetham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
Proteomic Analyses ◽  
Expression In Mammalian Cells
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