scholarly journals Cell adhesion profiling using extracellular matrix protein microarrays

BioTechniques ◽  
2006 ◽  
Vol 40 (4) ◽  
pp. 523-531 ◽  
Author(s):  
Cornelia Kuschel ◽  
Heiko Steuer ◽  
Andreas N. Maurer ◽  
Britta Kanzok ◽  
Reinout Stoop ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Matrix Protein ◽  
Protein Microarrays ◽  
Extracellular Matrix Protein

Tenascin is induced at implantation sites in the mouse uterus and interferes with epithelial cell adhesion

Development ◽  
1994 ◽  
Vol 120 (3) ◽  
pp. 661-671 ◽  
Author(s):  
J. Julian ◽  
R. Chiquet-Ehrismann ◽  
H.P. Erickson ◽  
D.D. Carson
Keyword(s):  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Early Pregnancy ◽  
Matrix Protein ◽  
Cellular Adhesion ◽  
Uterine Epithelium ◽  
Extracellular Matrix Protein ◽  
Mouse Uterus ◽  
Uterine Epithelial Cell

Expression of tenascin, an extracellular matrix protein associated with morphogenetic events and altered states of cellular adhesion, was examined in mouse uterus during the peri-implantation period. A uniform low level expression of tenascin was detected in stromal extracellular matrix during the estrous cycle and days 1 through 4 of early pregnancy. During the period of blastocyst attachment (day 4.5), an intense deposition of tenascin fibrils was located in the extracellular matrix of stroma immediately subjacent to the uterine epithelium surrounding the attaching blastocyst. This localized intensity of tenascin expression was both spatially and temporally restricted. By day 5.5, differentiation of stroma in the immediate area around the embryo to form the primary decidual zone was accompanied by a reduced amount of tenascin expression in the form of fragmented fibrils. Tenascin also could be induced by an artificial stimulus in uterine stroma of mice that had been hormonally prepared for implantation. The ability of artificial stimuli to induce tenascin expression suggested that the tenascin-inducing signals were derived from uterine cells, presumably lumenal epithelium, rather than embryonic cells. Consistent with this, conditioned medium from primary cultures of uterine epithelium was found to induce tenascin expression (2- to 4-fold) in isolated uterine stroma. Artificial stimuli generated a temporal pattern of tenascin expression similar to that observed during early pregnancy; however, in the artificially induced model, tenascin was induced in stroma immediately subjacent to lumenal epithelium along the entire length of the uterus. Purified tenascin and a recombinant tenascin fragment consisting of alternatively spliced fibronectin type III repeats, interfered with maintenance of uterine epithelial cell adhesion to Matrigel. In contrast, other recombinant tenascin fragments or fibronectin had no effect in this regard. Tenascin had no effect on adhesion of uterine stroma. Collectively, these results suggest that stimulation of TN expression in stromal extracellular matrix in vivo occurs via hormonally regulated, epithelial-mesenchymal interactions and serves as an early marker for uterine receptivity and the attachment phase of implantation. Furthermore, tenascin may facilitate embryo penetration by disrupting uterine epithelial cell adhesion to underlying basal lamina.

The extracellular matrix protein ?IG-H3 is expressed at myotendinous junctions and supports muscle cell adhesion

2003 ◽  
Vol 313 (1) ◽  
pp. 93-105 ◽  
Author(s):  
Jill W. Ferguson ◽  
Brian S. Thoma ◽  
Michelle F. Mikesh ◽  
Randall H. Kramer ◽  
Kelly L. Bennett ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Muscle Cell ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Myotendinous Junctions

Mode of Adsorption and Orientation of an Extracellular Matrix Protein Affect Its Cell-Adhesion-Promoting Activity

1998 ◽  
Vol 265 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Mark I. Fitchmun ◽  
Jutta Falk-Marzillier ◽  
Eldri Marshall ◽  
Gina Cruz ◽  
Jonathan C.R. Jones ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Matrix Protein ◽  
Extracellular Matrix Protein

Human Trophoblast Cell Adhesion to Extracellular Matrix Protein, Entactin

1996 ◽  
Vol 36 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Yan Yang ◽  
Jill C. Todt ◽  
David M. Svinarich ◽  
Faisal Qureshi ◽  
Suzanne M. Jacques ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Matrix Protein ◽  
Trophoblast Cell ◽  
Extracellular Matrix Protein ◽  
Human Trophoblast

scholarly journals Parallelized label-free monitoring of cell adhesion on extracellular matrix proteins measured by single colour reflectometry

2021 ◽  
Author(s):  
Johanna Hutterer ◽  
Günther Proll ◽  
Peter Fechner ◽  
Günter Gauglitz
Keyword(s):  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Matrix Protein ◽  
Morphological Changes ◽  
Extracellular Matrix Protein ◽  
High Time Resolution ◽  
Label Free ◽  
Implant Surface ◽  
Sensing Applications ◽  
Single Colour

AbstractThe understanding of the initial cell adhesion to biomaterials is crucial for the survival of implants. The manifold possibilities to tailor an implant surface and the diverse requirements for different implant applications necessitate a timesaving and highly parallelized analytical methodology. Due to its intrinsic advantages (label-free, time-resolved, robust against temperature fluctuations, and particularly the multiplexing possibilities), single colour reflectometry (SCORE) is used for the first time to investigate cell adhesion to different extracellular matrix protein–coated surfaces. The excellent correlation between the novel SCORE technology and well-established reference methods proves that the results obtained by using this direct optical method are able to reflect the cell binding processes at the transducer surface. Additionally, the high time resolution of SCORE revealed the differences in the adhesion behaviour of the cells on the different extracellular matrix protein–coated glass slides during the initial adsorption phase and during the spreading of the cells on the surfaces. Therefore, we conclude that SCORE is a perfectly suited methodology for studying the entire cell adsorption process, including morphological changes, and shows great potential for other cell-based sensing applications. Graphical abstract

1280: Increased Susceptibility to Genitovaginal Prolapse Associated with a Polymorphism in the Promoter of the Extracellular Matrix Protein LAMC1

2007 ◽  
Vol 177 (4S) ◽  
pp. 421-422
Author(s):  
Ganka Nikolova ◽  
Christian O. Twiss ◽  
Hane Lee ◽  
Nelson Stanley ◽  
Janet Sinsheimer ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Increased Susceptibility
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