scholarly journals Counter-selectable marker for bacterial-based interaction trap systems

BioTechniques ◽  
2006 ◽  
Vol 40 (2) ◽  
pp. 179-184 ◽  
Author(s):  
Xiangdong Meng ◽  
Robin M. Smith ◽  
Astrid V. Giesecke ◽  
J. Keith Joung ◽  
Scot A. Wolfe
Keyword(s):  
Selectable Marker ◽  
Interaction Trap

Rapid visualized assessment of drug efficacy in live mice with a selectable marker-free autoluminescent Klebsiella pneumoniae

2021 ◽  
Vol 177 ◽  
pp. 112919
Author(s):  
Xirong Tian ◽  
Yamin Gao ◽  
Shuai Wang ◽  
H.M. Adnan Hameed ◽  
Wei Yu ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Selectable Marker ◽  
Drug Efficacy ◽  
Marker Free

scholarly journals Choice of selectable marker affects recombinant protein expression in cells and exosomes

2021 ◽  
pp. 100838
Author(s):  
Chenxu Guo ◽  
Francis K. Fordjour ◽  
Shang Jui Tsai ◽  
James C. Morrell ◽  
Stephen J. Gould
Keyword(s):  
Recombinant Protein ◽  
Protein Expression ◽  
Selectable Marker ◽  
Recombinant Protein Expression ◽  
In Cells

Hygromycin B resistance as dominant selectable marker in yeast

1984 ◽  
Vol 8 (5) ◽  
pp. 353-358 ◽  
Author(s):  
Kevin R. Kaster ◽  
Stanley G. Burgett ◽  
Thomas D. Ingolia
Keyword(s):  
Selectable Marker ◽  
Hygromycin B ◽  
Hygromycin B Resistance ◽  
Dominant Selectable Marker

Use of a feedback-insensitive ? subunit of anthranilate synthase as a selectable marker for transformation of rice and potato

2004 ◽  
Vol 14 (4) ◽  
pp. 363-373 ◽  
Author(s):  
Tetsuya Yamada ◽  
Yuzuru Tozawa ◽  
Hisakazu Hasegawa ◽  
Teruhiko Terakawa ◽  
Yasunobu Ohkawa ◽  
...  
Keyword(s):  
Selectable Marker ◽  
Anthranilate Synthase

A high-level expression vector containing selectable marker for continuous production of recombinant protein in insect cells

2009 ◽  
Vol 31 (5) ◽  
pp. 623-627 ◽  
Author(s):  
Takuya Shishido ◽  
Naoya Kurata ◽  
Myung Eui Yoon ◽  
Tsutomu Tanaka ◽  
Hideki Yamaji ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Expression Vector ◽  
Insect Cells ◽  
Selectable Marker ◽  
Continuous Production ◽  
High Level Expression ◽  
High Level

A novel selectable marker based on Aspergillus niger arginase expression

2012 ◽  
Vol 51 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Kashyap Dave ◽  
Manmeet Ahuja ◽  
T.N. Jayashri ◽  
Rekha Bisht Sirola ◽  
Narayan S. Punekar
Keyword(s):  
Aspergillus Niger ◽  
Selectable Marker

scholarly journals Mammalian Protein-Protein Interaction Trap (MAPPIT) Analysis of STAT5, CIS, and SOCS2 Interactions with the Growth Hormone Receptor

2007 ◽  
Vol 21 (11) ◽  
pp. 2821-2831 ◽  
Author(s):  
Isabel Uyttendaele ◽  
Irma Lemmens ◽  
Annick Verhee ◽  
Anne-Sophie De Smet ◽  
Joël Vandekerckhove ◽  
...  
Keyword(s):  
Protein Interaction ◽  
Binding Sites ◽  
Growth Hormone Receptor ◽  
Functional Divergence ◽  
Critical Role ◽  
Sh2 Domain ◽  
Cytokine Signaling ◽  
Protein Protein Interaction ◽  
Interaction Trap ◽  
Mammalian Protein

Abstract Binding of GH to its receptor induces rapid phosphorylation of conserved tyrosine motifs that function as recruitment sites for downstream signaling molecules. Using mammalian protein-protein interaction trap (MAPPIT), a mammalian two-hybrid method, we mapped the binding sites in the GH receptor for signal transducer and activator of transcription 5 (STAT5) a and b and for the negative regulators of cytokine signaling cytokine-inducible Src-homology 2 (SH2)-containing protein (CIS) and suppressor of cytokine signaling 2 (SOCS2). Y534, Y566, and Y627 are the major recruitment sites for STAT5. A non-overlapping recruitment pattern is observed for SOCS2 and CIS with positions Y487 and Y595 as major binding sites, ruling out SOCS-mediated inhibition of STAT5 activation by competition for shared binding sites. More detailed analysis revealed that CIS binding to the Y595, but not to the Y487 motif, depends on both its SH2 domain and the C-terminal part of its SOCS box, with a critical role for the CIS Y253 residue. This functional divergence of the two CIS/SOCS2 recruitment sites is also observed upon substitution of the Y+1 residue by leucine, turning the Y487, but not the Y595 motif into a functional STAT5 recruitment site.

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