scholarly journals Membrane Potential Imaging with Voltage-sensitive Dye (VSD) for Long-term Recording

2021 ◽  
Vol 61 (6) ◽  
pp. 404-408
Author(s):  
Takashi TOMINAGA ◽  
Riichi KAJIWARA ◽  
Yoko TOMINAGA
Keyword(s):  
Membrane Potential ◽  
Voltage Sensitive Dye

Periodic Current Injection (PCI) -A New Method to Image Steady-State Membrane Potential of Single Neurons in situ Using Extracellular Voltage-Sensitive Dyes

1995 ◽  
Vol 50 (5-6) ◽  
pp. 435-438 ◽  
Author(s):  
Alexander Borst
Keyword(s):  
Steady State ◽  
Membrane Potential ◽  
Current Injection ◽  
New Method ◽  
Excitation Wavelength ◽  
Single Neurons ◽  
Voltage Sensitive Dye ◽  
Difference Image ◽  
Periodic Current

Abstract A new method is described which allows to image the steady-state distribution of m em ­ brane potential of single neurons in situ. The method consists of staining the tissue with an extracellular voltage-sensitive dye (Di-4-ANEPPS) and impaling a single neuron with a microelectrode. After focusing the imaging system onto the cell a large series of images are taken with a CCD camera at the appropriate excitation wavelength of the voltage-sensitive dye while the neuron’s membrane potential is shifted by a periodic current injection (PCI). Afterwards two groups of images are averaged separately: those images while the cell was at rest and those images while the cell was hyperpolarized. After subtraction of these averaged images, the resulting difference image shows only the membrane potential of the cell which was altered periodically. The success of the method is demonstrated on leech cells in intact ganglia. If applied to cells with a basically two-dimensional arborization pattern, the decrease of activity in the difference image in areas further away from the injection site should relate to a decrease in membrane potential according to the passive electrotonic properties of the cell under study.

Conditions for the Induction of Long-Term Potentiation and Long-Term Depression by Conjunctive Pairing in the Dentate Gyrus In Vitro

1997 ◽  
Vol 78 (5) ◽  
pp. 2569-2573 ◽  
Author(s):  
Yue Wang ◽  
Jianqun Wu ◽  
Michael J. Rowan ◽  
Roger Anwyl
Keyword(s):  
Membrane Potential ◽  
Dentate Gyrus ◽  
Kinase Inhibitor ◽  
Long Term Potentiation ◽  
Ruthenium Red ◽  
Long Term Depression ◽  
Afferent Stimulation ◽  
Term Potentiation

Wang, Yue, Jianqun Wu, Michael J. Rowan, and Roger Anwyl. Conditions for the induction of long-term potentiation and long-term depression by conjunctive pairing in the dentate gyrus in vitro. J. Neurophysiol. 78: 2569–2573, 1997. The conditions under which long-term potentiation (LTP) and long-term depression (LTD) of excitatory postsynaptic currents were induced by the conjunctive pairing-type protocol of afferent stimulation and postsynaptic depolarization were studied in the medial perforant pathway-granule cell synapse of the dentate gyrus in vitro. The conjunctive pairing of 1-Hz afferent stimulation and steady state postsynaptic depolarization to 0 mV did not induce LTP or LTD. Inhibition of LTD induction with a phosphatase inhibitor or ruthenium red resulted in induction of LTP after the conjunctive pairing. Such LTP induction was N-methyl-d-aspartate dependent. Conversely, inhibition of LTP induction with a kinase inhibitor resulted in LTD induction after the conjunctive pairing. Thus the failure to induce LTP or LTD with the pairing protocol involving depolarization to 0 mV membrane potential was due to simultaneous activation of intracellular processes that generate the induction of LTP and LTD. Increasing the frequency of afferent stimulation to 200 Hz, even for just eight stimuli, resulted in LTP induction. The studies show that two factors govern the induction of LTP/LTD, membrane potential and frequency of afferent stimulation, with either increased depolarization or increased afferent stimulation favoring LTP induction.

Optical recording of membrane potential in dissociated mouse vestibular ganglion cells using a voltage-sensitive dye

2000 ◽  
Vol 27 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Shi-Ming Yang ◽  
Tadashi Doi ◽  
Mikiya Asako ◽  
Ayumi Matsumoto ◽  
Toshio Yamashita
Keyword(s):  
Membrane Potential ◽  
Ganglion Cells ◽  
Optical Recording ◽  
Voltage Sensitive Dye ◽  
Vestibular Ganglion ◽  
Vestibular Ganglion Cells

Recording membrane potential changes through photoacoustic voltage sensitive dye

2017 ◽  
Author(s):  
Haichong K. Zhang ◽  
Jeeun Kang ◽  
Ping Yan ◽  
Diane S. Abou ◽  
Hanh N. D. Le ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Voltage Sensitive Dye

Accommodation of cat spinal motoneurones to linearly rising currents before and during long-term changes of membrane potential

1974 ◽  
Vol 76 (2) ◽  
pp. 213-221 ◽  
Author(s):  
W.R. Schlue ◽  
D.W. Richter ◽  
K.H. Mauritz ◽  
A.C. Nacimiento
Keyword(s):  
Membrane Potential ◽  
Long Term Changes ◽  
Spinal Motoneurones

How the Shape of Pre- and Postsynaptic Signals Can Influence STDP: A Biophysical Model

2004 ◽  
Vol 16 (3) ◽  
pp. 595-625 ◽  
Author(s):  
Ausra Saudargiene ◽  
Bernd Porr ◽  
Florentin Wörgötter
Keyword(s):  
Membrane Potential ◽  
Weight Change ◽  
Hebbian Learning ◽  
Dendritic Tree ◽  
Learning Rule ◽  
Long Term Potentiation ◽  
Spike Timing ◽  
Biophysical Model ◽  
Nmda Channel

Spike-timing-dependent plasticity (STDP) is described by long-term potentiation (LTP), when a presynaptic event precedes a postsynaptic event, and by long-term depression (LTD), when the temporal order is reversed. In this article, we present a biophysical model of STDP based on a differential Hebbian learning rule (ISO learning). This rule correlates presynaptically the NMDA channel conductance with the derivative of the membrane potential at the synapse as the postsynaptic signal. The model is able to reproduce the generic STDP weight change characteristic. We find that (1) The actual shape of the weight change curve strongly depends on the NMDA channel characteristics and on the shape of the membrane potential at the synapse. (2) The typical antisymmetrical STDP curve (LTD and LTP) can become similar to a standard Hebbian characteristic (LTP only) without having to change the learning rule. This occurs if the membrane depolarization has a shallow onset and is long lasting. (3) It is known that the membrane potential varies along the dendrite as a result of the active or passive backpropagation of somatic spikes or because of local dendritic processes. As a consequence, our model predicts that learning properties will be different at different locations on the dendritic tree. In conclusion, such site-specific synaptic plasticity would provide a neuron with powerful learning capabilities.

Nutrition and muscle potassium: differential effect in rat slow and fast muscles

1987 ◽  
Vol 65 (11) ◽  
pp. 2188-2190 ◽  
Author(s):  
C. N. Fong ◽  
H. L. Atwood ◽  
K. N. Jeejeebhoy ◽  
M. P. Charlton
Keyword(s):  
Membrane Potential ◽  
Soleus Muscle ◽  
Activity Pattern ◽  
Gastrocnemius Muscle ◽  
Hypocaloric Diet ◽  
Gastrocnemius Muscles ◽  
Fast Muscles ◽  
Slow And Fast Muscles

The effects of malnutrition on intracellular K+ activity, (aK)i, and membrane potential, Em, were measured by means of double-barrelled K+ -selective microlectrodes in the soleus and gastrocnemius muscles of the rat. (aK)i and Em were measured in vivo in normal anaesthetized animals and in rats subjected to one of two diet restrictions: a 2-day fast or a long-term hypocaloric diet. In the soleus muscle, (aK)i fell by similar amounts in both 2-day fasted and long-term hypocalorically fed rats, while Em depolarized significantly only in hypocalorically fed rats. In the gastrocnemius muscle, neither the 2-day fast nor the hypocaloric diet affected (aK)i or Em. It is suggested that the selective loss of K+ from the soleus muscle may be related to its activity pattern.

scholarly journals Evaluation of Lasting Effects of Heat Stress on Sperm Profile and Oxidative Status of Ram Semen and Epididymal Sperm

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Thais Rose dos Santos Hamilton ◽  
Camilla Mota Mendes ◽  
Letícia Signori de Castro ◽  
Patrícia Monken de Assis ◽  
Adriano Felipe Perez Siqueira ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heat Stress ◽  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Treated Group ◽  
Epididymal Sperm ◽  
Long Term Effects ◽  
Stressed Cells

Higher temperatures lead to an increase of testicular metabolism that results in spermatic damage. Oxidative stress is the main factor responsible for testicular damage caused by heat stress. The aim of this study was to evaluate lasting effects of heat stress on ejaculated sperm and immediate or long-term effects of heat stress on epididymal sperm. We observed decrease in motility and mass motility of ejaculated sperm, as well as an increase in the percentages of sperm showing major and minor defects, damaged plasma and acrosome membranes, and a decrease in the percentage of sperm with high mitochondrial membrane potential in the treated group until one spermatic cycle. An increased enzymatic activity of glutathione peroxidase and an increase of stressed cells were observed in ejaculated sperm of the treated group. A decrease in the percentage of epididymal sperm with high mitochondrial membrane potential was observed in the treated group. However, when comparing immediate and long-term effects, we observed an increase in the percentage of sperm with low mitochondrial membrane potential. In conclusion, testicular heat stress induced oxidative stress that led to rescuable alterations after one spermatic cycle in ejaculated sperm and also after 30 days in epididymal sperm.

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