scholarly journals Raman Optical Activity Probing the Active Site of Chromoproteins

2014 ◽  
Vol 54 (1) ◽  
pp. 039-042 ◽  
Author(s):  
Masashi UNNO
Keyword(s):  
Optical Activity ◽  
Active Site ◽  
Raman Optical Activity

Active Site Structure of Photoactive Yellow Protein with a Locked Chromophore Analogue Revealed by Near-Infrared Raman Optical Activity

2013 ◽  
Vol 4 (18) ◽  
pp. 3031-3038 ◽  
Author(s):  
Kensuke Kubota ◽  
Takahito Shingae ◽  
Nicole D. Foster ◽  
Masato Kumauchi ◽  
Wouter D. Hoff ◽  
...  
Keyword(s):  
Optical Activity ◽  
Active Site ◽  
Near Infrared ◽  
Photoactive Yellow Protein ◽  
Site Structure ◽  
Raman Optical Activity ◽  
Active Site Structure

Exploring the Active Site Structure of Photoreceptor Proteins by Raman Optical Activity

2010 ◽  
Author(s):  
Masashi Unno ◽  
Takahito Shingae ◽  
Takashi Kikukawa ◽  
Naoki Kamo ◽  
P. M. Champion ◽  
...  
Keyword(s):  
Optical Activity ◽  
Active Site ◽  
Site Structure ◽  
Raman Optical Activity ◽  
Active Site Structure

Exploring the Active Site Structure of a Photoreceptor Protein by Raman Optical Activity

2013 ◽  
Vol 117 (5) ◽  
pp. 1321-1325 ◽  
Author(s):  
Masashi Unno ◽  
Takashi Kikukawa ◽  
Masato Kumauchi ◽  
Naoki Kamo
Keyword(s):  
Optical Activity ◽  
Active Site ◽  
Site Structure ◽  
Raman Optical Activity ◽  
Active Site Structure

scholarly journals Spectroscopic ruler for measuring active-site distortions based on Raman optical activity of a hydrogen out-of-plane vibration

2018 ◽  
Vol 115 (35) ◽  
pp. 8671-8675 ◽  
Author(s):  
Shojiro Haraguchi ◽  
Takahito Shingae ◽  
Tomotsumi Fujisawa ◽  
Noritaka Kasai ◽  
Masato Kumauchi ◽  
...  
Keyword(s):  
Dihedral Angle ◽  
Optical Activity ◽  
Active Site ◽  
Active Sites ◽  
Density Functional ◽  
Water Soluble ◽  
Plane Vibration ◽  
Raman Optical Activity ◽  
Out Of Plane ◽  
Halorhodospira Halophila

Photoactive yellow protein (PYP), from the phototrophic bacterium Halorhodospira halophila, is a small water-soluble photoreceptor protein and contains p-coumaric acid (pCA) as a chromophore. PYP has been an attractive model for studying the physical chemistry of protein active sites. Here, we explore how Raman optical activity (ROA) can be used to extract quantitative information on distortions of the pCA chromophore at the active site in PYP. We use 13C8-pCA to assign an intense signal at 826 cm−1 in the ROA spectrum of PYP to a hydrogen out-of-plane vibration of the ethylenic moiety of the chromophore. Quantum-chemical calculations based on density functional theory demonstrate that the sign of this ROA band reports the direction of the distortion in the dihedral angle about the ethylenic C=C bond, while its amplitude is proportional to the dihedral angle. These results document the ability of ROA to quantify structural deformations of a cofactor molecule embedded in a protein moiety.

Structural characterization of proteins and viruses using Raman optical activity

2004 ◽  
Vol 35 (1-2) ◽  
pp. 87-92 ◽  
Author(s):  
Ewan W Blanch ◽  
Iain H McColl ◽  
Lutz Hecht ◽  
Kurt Nielsen ◽  
Laurence D Barron
Keyword(s):  
Optical Activity ◽  
Structural Characterization ◽  
Raman Optical Activity

scholarly journals Recognition of the true and false resonance Raman optical activity

2021 ◽  
Author(s):  
Malgorzata Baranska ◽  
Ewa Machalska ◽  
Grzegorz Zajac ◽  
Aleksandra J. Wierzba ◽  
Josef Kapitán ◽  
...  
Keyword(s):  
Optical Activity ◽  
Resonance Raman ◽  
Raman Optical Activity

scholarly journals Recognition of the true and false resonance Raman optical activity

2021 ◽  
Author(s):  
Malgorzata Baranska ◽  
Ewa Machalska ◽  
Grzegorz Zajac ◽  
Aleksandra J. Wierzba ◽  
Josef Kapitán ◽  
...  
Keyword(s):  
Optical Activity ◽  
Resonance Raman ◽  
Raman Optical Activity
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