scholarly journals 2SBP-03 A gel-encapsulated bioreactor system for NMR studies of protein-protein interactions in living mammalian cells(2SBP Transient macromolecular complexes involved in multilevel biological phenomena,Symposium,The 51th Annual Meeting of the Biophysical Society of Japan)

2013 ◽  
Vol 53 (supplement1-2) ◽  
pp. S97
Author(s):  
Noritaka Nishida ◽  
Ichio Shimada
Keyword(s):  
Annual Meeting ◽  
Protein Interactions ◽  
Mammalian Cells ◽  
Protein Protein Interactions ◽  
Nmr Studies ◽  
Macromolecular Complexes ◽  
Biological Phenomena ◽  
Biophysical Society ◽  
Bioreactor System

A Gel-Encapsulated Bioreactor System for NMR Studies of Protein-Protein Interactions in Living Mammalian Cells

2012 ◽  
Vol 125 (4) ◽  
pp. 1246-1249 ◽  
Author(s):  
Satoshi Kubo ◽  
Noritaka Nishida ◽  
Yuko Udagawa ◽  
Osamu Takarada ◽  
Shinji Ogino ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Mammalian Cells ◽  
Protein Protein Interactions ◽  
Nmr Studies ◽  
Bioreactor System

A Gel-Encapsulated Bioreactor System for NMR Studies of Protein-Protein Interactions in Living Mammalian Cells

2012 ◽  
Vol 52 (4) ◽  
pp. 1208-1211 ◽  
Author(s):  
Satoshi Kubo ◽  
Noritaka Nishida ◽  
Yuko Udagawa ◽  
Osamu Takarada ◽  
Shinji Ogino ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Mammalian Cells ◽  
Protein Protein Interactions ◽  
Nmr Studies ◽  
Bioreactor System

3P-290 Prediction of protein-protein interactions using domain sequences(The 46th Annual Meeting of the Biophysical Society of Japan)

2008 ◽  
Vol 48 (supplement) ◽  
pp. S172
Author(s):  
Masanori Kakuta ◽  
Kazuya Sumikoshi ◽  
Shugo Nakamura ◽  
Kentaro Shimizu
Keyword(s):  
Annual Meeting ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Biophysical Society

scholarly journals Applications of In-Cell NMR in Structural Biology and Drug Discovery

2019 ◽  
Vol 20 (1) ◽  
pp. 139 ◽  
Author(s):  
CongBao Kang
Keyword(s):  
Drug Discovery ◽  
Conformational Changes ◽  
Protein Interactions ◽  
Mammalian Cells ◽  
Structural Information ◽  
Living Cells ◽  
Bacterial Cells ◽  
Protein Protein Interactions ◽  
Nmr Studies ◽  
Post Translational Modifications

In-cell nuclear magnetic resonance (NMR) is a method to provide the structural information of a target at an atomic level under physiological conditions and a full view of the conformational changes of a protein caused by ligand binding, post-translational modifications or protein–protein interactions in living cells. Previous in-cell NMR studies have focused on proteins that were overexpressed in bacterial cells and isotopically labeled proteins injected into oocytes of Xenopus laevis or delivered into human cells. Applications of in-cell NMR in probing protein modifications, conformational changes and ligand bindings have been carried out in mammalian cells by monitoring isotopically labeled proteins overexpressed in living cells. The available protocols and successful examples encourage wide applications of this technique in different fields such as drug discovery. Despite the challenges in this method, progress has been made in recent years. In this review, applications of in-cell NMR are summarized. The successful applications of this method in mammalian and bacterial cells make it feasible to play important roles in drug discovery, especially in the step of target engagement.

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