scholarly journals 1PT116 Crystal structure of the fatty acid binding protein 3 (FABP3) with or without fatty acid(The 50th Annual Meeting of the Biophysical Society of Japan)

2012 ◽  
Vol 52 (supplement) ◽  
pp. S88
Author(s):  
Mika Hirose ◽  
Shigeru Sugiyama ◽  
Mayumi Niiyama ◽  
Hanako Ishida ◽  
Tsuyoshi Inoue ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Fatty Acid ◽  
Annual Meeting ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Biophysical Society

The crystal structure of Echinococcus granulosus fatty-acid-binding protein 1

2003 ◽  
Vol 1649 (1) ◽  
pp. 40-50 ◽  
Author(s):  
Emma Jakobsson ◽  
Gabriela Alvite ◽  
Terese Bergfors ◽  
Adriana Esteves ◽  
Gerard J. Kleywegt
Keyword(s):  
Crystal Structure ◽  
Fatty Acid ◽  
Echinococcus Granulosus ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

scholarly journals Crystal Structure and Thermodynamic Analysis of Human Brain Fatty Acid-binding Protein

2000 ◽  
Vol 275 (35) ◽  
pp. 27045-27054
Author(s):  
Ganesaratnam K. Balendiran ◽  
Frank Schnütgen ◽  
Giovanna Scapin ◽  
Torsten Börchers ◽  
Ning Xhong ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Fatty Acid ◽  
Human Brain ◽  
Thermodynamic Analysis ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Crystal Structure of Chicken Liver Basic Fatty Acid-Binding Protein Complexed with Cholic Acid†,‡

Biochemistry ◽  
2004 ◽  
Vol 43 (44) ◽  
pp. 14072-14079 ◽  
Author(s):  
Daniele Nichesola ◽  
Massimiliano Perduca ◽  
Stefano Capaldi ◽  
Maria E. Carrizo ◽  
Pier Giorgio Righetti ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Fatty Acid ◽  
Cholic Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Chicken Liver ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Crystal structure of chicken liver basic fatty acid-binding protein at 2.7 � resolution

1990 ◽  
Vol 98 (1-2) ◽  
Author(s):  
Giovanna Scapin ◽  
Paola Spadon ◽  
Mario Mammi ◽  
Giuseppe Zanotti ◽  
HugoL. Monaco
Keyword(s):  
Crystal Structure ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Chicken Liver ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Expression, Purification, and Crystal Structure Determination of Recombinant Human Epidermal-Type Fatty Acid Binding Protein†,‡

Biochemistry ◽  
1999 ◽  
Vol 38 (38) ◽  
pp. 12229-12239 ◽  
Author(s):  
Carsten Hohoff ◽  
Torsten Börchers ◽  
Bernd Rüstow ◽  
Friedrich Spener ◽  
Herman van Tilbeurgh
Keyword(s):  
Crystal Structure ◽  
Fatty Acid ◽  
Structure Determination ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Crystal Structure Determination ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Crystal structure of human brain-type fatty acid-binding protein FABP7 complexed with palmitic acid

2021 ◽  
Vol 77 (7) ◽  
Author(s):  
Ki Hyun Nam
Keyword(s):  
Crystal Structure ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Human Brain ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Binding Pocket ◽  
Aliphatic Chain ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

The brain-type fatty acid-binding protein FABP7, which is expressed in astrocytes and neural progenitors, is a member of the intracellular lipid-binding protein family. This protein is not only involved in various cellular functions such as metabolism, inflammation and energy homeostasis, but also in diseases such as cognitive disorders and tumors. Structures of unsaturated fatty acids, such as oleic acid (OA) and docosahexaenoic acid (DHA), bound to FABP7 have been elucidated; however, structures of saturated fatty acids bound to FABP7 remain unknown. To better understand fatty acid recognition, here the crystal structure of human brain-type fatty acid-binding protein FABP7 complexed with palmitic acid (PA), a saturated fatty acid, is reported at a resolution of 1.6 Å. The PA bound to the fatty acid-binding pocket of FABP7 assumed a U-shaped conformation. The carboxylate moiety of PA interacted with Tyr129, Arg127 and, via a water bridge, with Arg107 and Thr54, whereas its aliphatic chain was stabilized by hydrophobic interactions with Met21, Leu24, Thr30, Thr37, Pro39, Phe58 and Asp77. Structural comparison showed that PA, OA and DHA exhibited unique binding conformations in the fatty acid-binding pocket, stabilized by distinct amino-acid interactions. The binding of PA to FABP7 exhibits a unique binding conformation when compared with other human FABPs (FABP3–FABP5 and FABP8) expressed in other tissues. Based on the crystal and fatty acid structures, it was suggested that PA, which prefers a linear form in nature, required a greater conformational change in its aliphatic chain to bind to the fatty acid-binding pocket in a U-shaped conformation, compared with the cis configurations of OA or DHA. This, together with the length of the aliphatic chain, was considered to be one of the factors determining the binding affinity of PA to FABP7. These results provide a better understanding of fatty acid recognition by FABP7 and expand the knowledge of the binding of PA to FABPs.

Sign in / Sign up

Export Citation Format

Share Document