scholarly journals 3I1058 Functional Implications from Structural Characterization of CN-bound Nitric Oxide Reductase(Heme Proteins,Oral Presentation)

2012 ◽  
Vol 52 (supplement) ◽  
pp. S73
Author(s):  
Takehiko Tosha ◽  
Nozomi Sato ◽  
Norihiro Okada ◽  
Hiroshi Sugimoto ◽  
Yoshitsugu Shiro
Keyword(s):  
Nitric Oxide ◽  
Oral Presentation ◽  
Structural Characterization ◽  
Heme Proteins ◽  
Nitric Oxide Reductase ◽  
Functional Implications

Kinetic Characterization of the Escherichia coli Nitric Oxide Reductase Flavorubredoxin

2008 ◽  
pp. 47-62 ◽  
Author(s):  
João B. Vicente ◽  
Francesca M. Scandurra ◽  
Elena Forte ◽  
Maurizio Brunori ◽  
Paolo Sarti ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Escherichia Coli ◽  
Kinetic Characterization ◽  
Nitric Oxide Reductase

Structural and Functional Characterization of Nitric Oxide Reductase

1997 ◽  
Vol 7 (C2) ◽  
pp. C2-587-C2-591 ◽  
Author(s):  
Y. Shiro ◽  
K. Tsukamoto ◽  
E. Obayashi ◽  
S. I. Adachi ◽  
T. Iizuka ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Functional Characterization ◽  
Nitric Oxide Reductase

scholarly journals Characterization of the norB Gene, Encoding Nitric Oxide Reductase, in the Nondenitrifying Cyanobacterium Synechocystis sp. Strain PCC6803

2002 ◽  
Vol 68 (2) ◽  
pp. 668-672 ◽  
Author(s):  
Andrea B�sch ◽  
B�rbel Friedrich ◽  
Rainer Cramm
Keyword(s):  
Nitric Oxide ◽  
Reductase Activity ◽  
Nitric Oxide Reductase ◽  
Wild Type ◽  
Gene Encoding ◽  
Single Subunit ◽  
Transcriptional Fusions ◽  
Synechocystis Sp ◽  
Negative Mutant

ABSTRACT A norB gene encoding a putative nitric oxide reductase is present in the genome of the nondenitrifying cyanobacterium Synechocystis sp. strain PCC6803. The gene product belongs to the quinol-oxidizing single-subunit class of nitric oxide reductases, discovered recently in the denitrifier Ralstonia eutropha. Heterologous complementation of a nitric oxide reductase-negative mutant of R. eutropha with norB from Synechocystis restored nitric oxide reductase activity. With reduced menadione as the electron donor, an enzymatic activity of 101 nmol of NO per min per mg of protein was obtained with membrane fractions of Synechocystis wild-type cells. Virtually no nitric oxide reductase activity was present in a norB-negative mutant of Synechocystis. Growing cells of this mutant are more sensitive toward NO than wild-type cells, indicating that the presence of a nitric oxide reductase is beneficial for Synechocystis when the cells are exposed to NO. Transcriptional fusions with the chloramphenicol acetyltransferase reporter gene were constructed to monitor norB expression in Synechocystis. Transcription of norB was not enhanced by the addition of the NO-generating agent sodium nitroprusside.

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