scholarly journals 1SP7-08 Computational investigations of mechanisms of enzymatic reactions of RNA-binding proteins(1SP7 Elucidation of Protein Functions at the Atomic Level with X-ray structural, Vibrational spectroscopic, Molecular biological and Theoretical analyses,The 47th Annual Meeting of the Biophysical Society of Japan)

2009 ◽  
Vol 49 (supplement) ◽  
pp. S9-S10
Author(s):  
Masaru Tateno ◽  
Shigehide Kuroyanagi ◽  
Yohsuke Hagiwara
Keyword(s):  
Annual Meeting ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Atomic Level ◽  
Enzymatic Reactions ◽  
X Ray ◽  
Biophysical Society ◽  
Protein Functions ◽  
Theoretical Analyses

scholarly journals The emerging universe of RNA-binding proteins

2015 ◽  
Vol 37 (2) ◽  
pp. 33-38
Author(s):  
Alfredo Castello
Keyword(s):  
Rna Synthesis ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Biological Processes ◽  
Quantitative Mass Spectrometry ◽  
Rna Biology ◽  
Protein Functions ◽  
Broad Variety ◽  
Selection Of

RNA associates with RNA-binding proteins (RBPs) from synthesis to decay, forming dynamic ribonucleoproteins that orchestrate gene expression. RBPs mediate crucial functions in RNA metabolism, including RNA synthesis, processing, transport, translation and degradation. Although essential for RNA life cycle, the repertoire of RBPs has remained largely unknown. The very recent development of a novel proteomic-based system-wide approach termed RNA interactome capture revealed the near-complete census of human RBPs. Applying UV cross-linking of proteins to RNA, oligo(dT) selection of poly(A) RNAs and quantitative mass spectrometry, this method not only ‘rediscovered’ most of the proteins already known to bind RNA, but added hundreds of novel members to the previously known repertoire of RBPs. The newly identified RBPs are distributed over a broad variety of protein families, participate in different biological processes and exert distinct protein functions, implying the existence of unprecedented links between RNA biology and intermediary metabolism, signalling, cellular homoeostasis or disease. Although the near-complete atlas of RBPs offered important insights into RNA biology, it also opened new functional and structural questions about protein–RNA interactions that remain to be answered.

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