scholarly journals 1P104 Design of a soluble transmembrane helix for measurement of water-membrane partitioning(Membrane proteins,Oral Presentations)

2007 ◽  
Vol 47 (supplement) ◽  
pp. S49
Author(s):  
Norie Shimai ◽  
Yoshiaki Yano ◽  
Katsumi Matsuzaki
Keyword(s):  
Membrane Proteins ◽  
Transmembrane Helix ◽  
Membrane Partitioning ◽  
Oral Presentations

Specificity and promiscuity in membrane helix interactions

1994 ◽  
Vol 27 (2) ◽  
pp. 157-218 ◽  
Author(s):  
Mark A. Lemmon ◽  
Donald M. Engelman
Keyword(s):  
Membrane Proteins ◽  
Transmembrane Helix ◽  
Integral Membrane Proteins ◽  
Lipid Packing ◽  
Membrane Protein Folding ◽  
Membrane Spanning ◽  
Α Helix ◽  
Prosthetic Groups ◽  
Packing Effects ◽  
Helix Interactions

The membrane-spanning portions of many integral membrane proteins consist of one or a number of transmembrane α-helices, which are expected to be independently stable on thermodynamic grounds. Side-by-side interactions between these transmembrane α-helices are important in the folding and assembly of such integral membrane proteins and their complexes. In considering the contribution of these helix–helix interactions to membrane protein folding and oligomerization, a distinction between the energetics and specificity should be recognized. A number of contributions to the energetics of transmembrane helix association within the lipid bilayer will be relatively non-specific, including those resulting from charge–charge interactions and lipid–packing effects. Specificity (and part of the energy) in transmembrane α-helix association, however, appears to rely mainly upon a detailed stereochemical fit between sets of dynamically accessible states of particular helices. In some cases, these interactions are mediated in part by prosthetic groups.

scholarly journals FOLDING ELASTIC TRANSMEMBRANE HELICES TO FIT IN A LOW-RESOLUTION IMAGE BY ELECTRON MICROSCOPY

2011 ◽  
Vol 09 (supp01) ◽  
pp. 37-50 ◽  
Author(s):  
YUTAKA UENO ◽  
KAZUNORI KAWASAKI ◽  
OSAMU SAITO ◽  
MASAFUMI ARAI ◽  
MAKIKO SUWA
Keyword(s):  
Membrane Proteins ◽  
Structure Prediction ◽  
Molecular Model ◽  
Imaging Techniques ◽  
Transmembrane Helix ◽  
Prediction Method ◽  
Molecular Shape ◽  
Coarse Grained ◽  
Transmembrane Helices ◽  
Low Resolution

Structure prediction of membrane proteins could be constrained and thereby improved by introducing data of the observed molecular shape. We studied a coarse-grained molecular model that relied on residue-based dummy atoms to fold the transmembrane helices of a protein in the observed molecular shape. Based on the inter-residue potential, the α-helices were folded to contact each other in a simulated annealing protocol to search optimized conformation. Fitting the model into a three-dimensional volume was tested for proteins with known structures and resulted in a fairly reasonable arrangement of helices. In addition, the constraint to the packing transmembrane helix with the two-dimensional region was tested and found to work as a very similar folding guide. The obtained models nicely represented α-helices with the desired slight bend. Our structure prediction method for membrane proteins well demonstrated reasonable folding results using a low-resolution structural constraint introduced from recent cell-surface imaging techniques.

scholarly journals 1P115 Crystal structure of the MgtE Mg^ transporter(Membrane proteins,Oral Presentations)

2007 ◽  
Vol 47 (supplement) ◽  
pp. S52
Author(s):  
Motoyuki Hattori ◽  
Yoshiki Tanaka ◽  
Shuya Fukai ◽  
Ryuichiro Ishitani ◽  
Osamu Nureki
Keyword(s):  
Crystal Structure ◽  
Membrane Proteins ◽  
Oral Presentations

scholarly journals Design of Soluble Transmembrane Helix for Measurement of Water-Membrane Partitioning

2009 ◽  
Vol 96 (3) ◽  
pp. 336a
Author(s):  
Yoshiaki Yano ◽  
Norie Shimai ◽  
Katsumi Matsuzaki
Keyword(s):  
Transmembrane Helix ◽  
Membrane Partitioning

scholarly journals 1P110 2D-crystallization of bovine heart NADH-ubiquinone oxidoreductase(Membrane proteins,Oral Presentations)

2007 ◽  
Vol 47 (supplement) ◽  
pp. S51
Author(s):  
Satoko Amano ◽  
Masakazu Moriyama ◽  
Yumiko Nakashima ◽  
Kyoko Shinzawa-Itoh ◽  
Christoph Gerle ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Ubiquinone Oxidoreductase ◽  
Bovine Heart ◽  
Nadh Ubiquinone Oxidoreductase ◽  
Oral Presentations

scholarly journals Helix Packing in Polytopic Membrane Proteins: Role of Glycine in Transmembrane Helix Association

1999 ◽  
Vol 77 (3) ◽  
pp. 1609-1618 ◽  
Author(s):  
Maryam M. Javadpour ◽  
Markus Eilers ◽  
Michel Groesbeek ◽  
Steven O. Smith
Keyword(s):  
Membrane Proteins ◽  
Transmembrane Helix ◽  
Helix Packing

scholarly journals N-terminal transmembrane-helix epitope tag for x-ray crystallography and electron microscopy of small membrane proteins

2021 ◽  
pp. 166909
Author(s):  
Benjamin C. McIlwain ◽  
Amanda L. Erwin ◽  
Alexander R. Davis ◽  
B. Ben Koff ◽  
Louise Chang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Membrane Proteins ◽  
Transmembrane Helix ◽  
Epitope Tag ◽  
X Ray ◽  
X Ray Crystallography
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