scholarly journals 1P089 Structural analysis of transmembrane halobacterial tranceducer pHtrII by multi-dimensional high-resolution solid-state NMR(Membrane proteins,Poster Presentations)

2007 ◽  
Vol 47 (supplement) ◽  
pp. S45
Author(s):  
Ayako Egawa ◽  
Miwako Saeki ◽  
Kokoro Hayashi ◽  
Chojiro Kojima ◽  
Hideo Akutsu ◽  
...  
Keyword(s):  
Solid State ◽  
Structural Analysis ◽  
High Resolution ◽  
Membrane Proteins ◽  
Solid State Nmr ◽  
Poster Presentations

Solid state NMR of membrane proteins: methods and applications

2021 ◽  
Author(s):  
Vivien Yeh ◽  
Boyan B. Bonev
Keyword(s):  
Solid State ◽  
High Resolution ◽  
Membrane Proteins ◽  
Solid State Nmr ◽  
Lipid Membrane ◽  
Cell Function ◽  
Membrane Lipid ◽  
Pharmacological Intervention ◽  
Nmr Methods ◽  
Informative Approach

Membranes of cells are active barriers, in which membrane proteins perform essential remodelling, transport and recognition functions that are vital to cells. Membrane proteins are key regulatory components of cells and represent essential targets for the modulation of cell function and pharmacological intervention. However, novel folds, low molarity and the need for lipid membrane support present serious challenges to the characterisation of their structure and interactions. We describe the use of solid state NMR as a versatile and informative approach for membrane and membrane protein studies, which uniquely provides information on structure, interactions and dynamics of membrane proteins. High resolution approaches are discussed in conjunction with applications of NMR methods to studies of membrane lipid and protein structure and interactions. Signal enhancement in high resolution NMR spectra through DNP is discussed as a tool for whole cell and interaction studies.

scholarly journals Structural Analysis of Transmembrane Helical Halobacterial Transducer pHtrll by High-Resolution Solid-State NMR

2003 ◽  
Vol 43 (supplement) ◽  
pp. S184
Author(s):  
Y. Matsuki ◽  
Y. Sudo ◽  
C. Kojima ◽  
T. Fujiwara ◽  
N. Kamo ◽  
...  
Keyword(s):  
Solid State ◽  
Structural Analysis ◽  
High Resolution ◽  
Solid State Nmr

scholarly journals 2P020 Secondary Structural Analysis of H^+-ATP Synthase Subunit c by High-Resolution Solid-State NMR

2004 ◽  
Vol 44 (supplement) ◽  
pp. S114
Author(s):  
M. Kobayashi ◽  
Y. Matsuki ◽  
T. Fujiwara ◽  
H. Akutsu
Keyword(s):  
Solid State ◽  
Structural Analysis ◽  
High Resolution ◽  
Atp Synthase ◽  
Solid State Nmr ◽  
Subunit C

scholarly journals Membrane Protein Structure Determination and Characterisation by Solution and Solid-State NMR

Biology ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 396
Author(s):  
Vivien Yeh ◽  
Alice Goode ◽  
Boyan B. Bonev
Keyword(s):  
Protein Structure ◽  
Solid State ◽  
High Resolution ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Solid State Nmr ◽  
Basic Unit ◽  
Molecular Assemblies ◽  
Nmr Studies ◽  
Main Challenge

Biological membranes define the interface of life and its basic unit, the cell. Membrane proteins play key roles in membrane functions, yet their structure and mechanisms remain poorly understood. Breakthroughs in crystallography and electron microscopy have invigorated structural analysis while failing to characterise key functional interactions with lipids, small molecules and membrane modulators, as well as their conformational polymorphism and dynamics. NMR is uniquely suited to resolving atomic environments within complex molecular assemblies and reporting on membrane organisation, protein structure, lipid and polysaccharide composition, conformational variations and molecular interactions. The main challenge in membrane protein studies at the atomic level remains the need for a membrane environment to support their fold. NMR studies in membrane mimetics and membranes of increasing complexity offer close to native environments for structural and molecular studies of membrane proteins. Solution NMR inherits high resolution from small molecule analysis, providing insights from detergent solubilised proteins and small molecular assemblies. Solid-state NMR achieves high resolution in membrane samples through fast sample spinning or sample alignment. Recent developments in dynamic nuclear polarisation NMR allow signal enhancement by orders of magnitude opening new opportunities for expanding the applications of NMR to studies of native membranes and whole cells.

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