scholarly journals 2P244 Membrane hyperpolarization induced membrane fluidization in Paramecium : Correlation with the regulation of ciliary beat frequency(39. Cell motility,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

2006 ◽  
Vol 46 (supplement2) ◽  
pp. S356
Author(s):  
yasuo nakaoka ◽  
junya uemura
Keyword(s):  
Cell Motility ◽  
Poster Session ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Membrane Hyperpolarization ◽  
Induced Membrane ◽  
Meeting Program ◽  
Membrane Fluidization ◽  
Ciliary Beat

Effects of Prostaglandins D, and E2on Ciliary Beat Frequency of Human Upper Respiratory Ciliain Vitro

1995 ◽  
Vol 115 (3) ◽  
pp. 438-442 ◽  
Author(s):  
P. J. Schuil ◽  
M. Ten Berge ◽  
J. M. E. Van Gelder ◽  
K. Graamans ◽  
E. H. Huizing
Keyword(s):  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Upper Respiratory ◽  
Ciliary Beat

Apparatus for endoscopical, laser-based determination of ciliary beat frequency

2005 ◽  
Author(s):  
N. Bogdanovic ◽  
B. Krattiger ◽  
J. Ricka ◽  
M. Frenz
Keyword(s):  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Ciliary Beat

Betadine has a ciliotoxic effect on ciliated human respiratory cells

2014 ◽  
Vol 129 (S1) ◽  
pp. S45-S50 ◽  
Author(s):  
J H Kim ◽  
J Rimmer ◽  
N Mrad ◽  
S Ahmadzada ◽  
R J Harvey
Keyword(s):  
Epithelial Cells ◽  
High Speed ◽  
Imaging System ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Positive Control ◽  
Clinical Dose ◽  
Significant Difference ◽  
Sinonasal Mucosa ◽  
Ciliary Beat

AbstractObjective:This study investigated the effect of Betadine on ciliated human respiratory epithelial cells.Methods:Epithelial cells from human sinonasal mucosa were cultured at the air–liquid interface. The cultures were tested with Hanks' balanced salt solution containing 10 mM HEPES (control), 100 µM ATP (positive control), 5 per cent Betadine or 10 per cent Betadine (clinical dose). Ciliary beat frequency was analysed using a high-speed camera on a computer imaging system.Results:Undiluted 10 per cent Betadine (n = 6) decreased the proportion of actively beating cilia over 1 minute (p < 0.01). Ciliary beat frequency decreased from 11.15 ± 4.64 Hz to no detectable activity. The result was similar with 5 per cent Betadine (n = 7), with no significant difference compared with the 10 per cent solution findings.Conclusion:Betadine, at either 5 and 10 per cent, was ciliotoxic. Caution should be applied to the use of topical Betadine solution on the respiratory mucosal surface.

Correlations among Mucociliary Transport, Ciliary Function, and Ciliary Structure

1998 ◽  
Vol 12 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Mark Jorissen
Keyword(s):  
Defense Mechanisms ◽  
Ex Vivo ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Preliminary Evidence ◽  
Relevant Parameter ◽  
Mucociliary Transport ◽  
Ciliary Dyskinesia ◽  
Ciliary Beat

Mucociliary transport is one of the most important defense mechanisms of the airway. Mucociliary transport time or rate, as measured using the saccharin test or the radioisotope technique, respectively, is clinically the most relevant parameter, although subject to large intra- and interindividual variability. There is no correlation between mucociliary transport in vivo and ciliary beat frequency ex vivo. Preliminary evidence demonstrates that mucociliary transport correlates with ciliary structure and orientation as investigated with transmission and scanning electron microscopy. A correlation is presented between ciliary beat frequency and secondary ciliary abnormalities. This correlation can best be described according to the logistic sigmoid model (r = 0.69). Based on these functional data, an ultrastructural distinction is proposed among normal (less than 5%), light (5 to 15%), moderate (15 to 25%), and severe (more than 25%) secondary ciliary dyskinesia.

Luffa Operculata Affects Mucociliary Function of the Isolated Frog Palate

2005 ◽  
Vol 19 (4) ◽  
pp. 353-357 ◽  
Author(s):  
Mônica Aidar Menon-Miyake ◽  
Regiani Carvalho de Oliveira ◽  
Geraldo Lorenzi-Filho ◽  
Paulo Hilário Nascimento Saldiva ◽  
Ossamu Butugan
Keyword(s):  
Alternative Medicine ◽  
Nasal Mucosa ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
The United States ◽  
Mucociliary Transport ◽  
Transport Velocity ◽  
Mucociliary Function ◽  
Frog Palate ◽  
Ciliary Beat

Background Luffa operculata is a medicinal plant used in homeopathic and alternative medicine. In the United States, it is sold in a purified spray form, whereas a homemade L. operculata dry fruit infusion (DFI) is commonly used in Latin America. The L. operculata DFI is applied intranasally, inducing profuse mucous secretion and relieving nasal symptoms. Nevertheless, this medication may cause irritation of the nasal mucosa, as well as epistaxis or anosmia. Given the growing popularity of alternative medicine, a decision was made to evaluate the effects of this substance on mucous membranes. Methods The effects of L. operculata DFI on mucociliary transport velocity, ciliary beat frequency, and transepithelial potential difference (PD) were evaluated in an isolated frog palate preparation. We tested 46 palates immediately before immersion and again at 5 and 20 minutes after immersion. Four groups (n = 10) were tested in frog Ringer: control; L. operculata DFI, 60 mg/L; 600 mg/L; and 1200 mg/L. An additional group was tested using L. operculata DFI prepared with water (600 mg/L of H2O, n = 6). Epithelial samples were harvested for ultrastructural study. Results In treated palates, mucociliary transport velocity and ciliary beat frequency decreased significantly (p < 0.001 and p < 0.008, respectively). There was a dose-dependent decrease in PD modulus (p < .007). Our PD findings indicated ion-fluid transport abnormalities, which were confirmed by transmission electron microscopy that showed enlargement of interepithelial spaces. Conclusion In this ex vivo model, the L. operculata DFI infusion promoted significant changes in the mucociliary function of the epithelium, suggesting that it is potentially noxious to human nasal mucosa.

Cellular-Neural-Cellular Pathways Mediating the Response of Tracheal Ciliary Beat Frequency (CBFt) to Inhaled Capsaicin

CHEST Journal ◽  
1992 ◽  
Vol 101 (3) ◽  
pp. 72S-73S ◽  
Author(s):  
Donovan B. Yeates ◽  
Mohammed Eljamal ◽  
Lid B. Wong ◽  
Irving F. Miller
Keyword(s):  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Cellular Pathways ◽  
Ciliary Beat

Capsaicin-activated bronchial- and alveolar-initiated pathways regulating tracheal ciliary beat frequency

1994 ◽  
Vol 77 (3) ◽  
pp. 1239-1245 ◽  
Author(s):  
M. Eljamal ◽  
L. B. Wong ◽  
D. B. Yeates
Keyword(s):  
Low Dose ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Neural Stimulation ◽  
Short Exposure ◽  
Tracheal Lumen ◽  
Prolonged Stimulation ◽  
Prior Administration ◽  
Stimulation Of ◽  
Ciliary Beat

We questioned whether the prolonged stimulation of ciliary beat frequency (CBF) to a short exposure of low-dose capsaicin (Wong et al. J. Appl. Physiol. 68: 257–2580, 1990) could be due to the activation of indirect pathways involving neural reflexes initiated independently in the bronchi and alveoli. Tracheal CBF (CBFtr) was measured temporally in anesthetized groups of 10 dogs by means of heterodyne-mode correlation analysis laser light scattering. To elucidate the site of the afferent neural stimulation and the efferent mediators affecting the ciliated epithelium, capsaicin (3 nM) aerosol was delivered for 4 min, either predominantly to the bronchi or to the alveolar regions, with use of pulsed aerosol techniques. This resulted in 13 pg of bronchial (85%) and 10 pg of alveolar (96%) capsaicin deposited, which caused marked stimulation of CBFtr with maxima at 7 and 35 min, respectively. Prior administration of aerosolized indomethacin to the bronchi or aerosolized cromolyn to the alveoli inhibited the bronchial and alveolar responses, respectively. Prior administration of aerosolized hexamethonium to the tracheal lumen blocked the stimulatory CBFtr responses from both capsaicin challenges. Ipratropium or propranolol aerosols delivered to the tracheal lumen also inhibited these responses. It is proposed that these pathways comprise one set of sensitive mechanisms to ensure a prolonged stimulation of CBF to effect the removal of secretions and the irritant from the lungs.

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