scholarly journals 3P212 A cell culture condition which prevents anoikis of anchorage dependent cells

2005 ◽  
Vol 45 (supplement) ◽  
pp. S256
Author(s):  
Y. Emoto
2004 ◽  
Vol 44 (supplement) ◽  
pp. S162
Author(s):  
Y. Emoto ◽  
C. Imafuku ◽  
K. Tawada

2011 ◽  
Vol 27 (9) ◽  
pp. 907 ◽  
Author(s):  
Xiaofang GAO ◽  
Yo TANAKA ◽  
Yasuhiko SUGII ◽  
Kazuma MAWATARI ◽  
Takehiko KITAMORI

2012 ◽  
Vol 87 (Suppl_1) ◽  
pp. 588-588
Author(s):  
Kyung-Jun Uh ◽  
Se-Heon Jeong ◽  
Chi Jeon Yang ◽  
Eui-Bae Jeung ◽  
Sang-Hwan Hyun ◽  
...  

2020 ◽  
Vol 119 (1) ◽  
pp. 118-127
Author(s):  
Valeria Franchini ◽  
Thomas Müller ◽  
Julian M. Haupt ◽  
Patrick Ostheim ◽  
Matthaeus Majewski ◽  
...  

2013 ◽  
Vol 65 (2) ◽  
pp. 219-225
Author(s):  
Norbert Nass ◽  
Kristian Weissenberg ◽  
Veronika Somoza ◽  
Stefanie Ruhs ◽  
Rolf-Edgar Silber ◽  
...  

2018 ◽  
Vol 70 (3) ◽  
pp. 285-293 ◽  
Author(s):  
Jiaojiao Zhang ◽  
Francesca Giampieri ◽  
Sadia Afrin ◽  
Maurizio Battino ◽  
Xiaodong Zheng ◽  
...  

2009 ◽  
Vol 108 ◽  
pp. S157-S158
Author(s):  
Shinji Sugiura ◽  
Koji Hattori ◽  
Toshiyuki Kanamori

Author(s):  
W. Shain ◽  
H. Ancin ◽  
H.C. Craighead ◽  
M. Isaacson ◽  
L. Kam ◽  
...  

Neural protheses have potential to restore nervous system functions lost by trauma or disease. Nanofabrication extends this approach to implants for stimulating and recording from single or small groups of neurons in the spinal cord and brain; however, tissue compatibility is a major limitation to their practical application. We are using a cell culture method for quantitatively measuring cell attachment to surfaces designed for nanofabricated neural prostheses.Silicon wafer test surfaces composed of 50-μm bars separated by aliphatic regions were fabricated using methods similar to a procedure described by Kleinfeld et al. Test surfaces contained either a single or double positive charge/residue. Cyanine dyes (diIC18(3)) stained the background and cell membranes (Fig 1); however, identification of individual cells at higher densities was difficult (Fig 2). Nuclear staining with acriflavine allowed discrimination of individual cells and permitted automated counting of nuclei using 3-D data sets from the confocal microscope (Fig 3). For cell attachment assays, LRM5 5 astroglial cells and astrocytes in primary cell culture were plated at increasing cell densities on test substrates, incubated for 24 hr, fixed, stained, mounted on coverslips, and imaged with a 10x objective.


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