scholarly journals High-throughput and high-precision structure determination in protein crystallography

2003 ◽  
Vol 43 (supplement) ◽  
pp. S1
Author(s):  
K. Miki
Keyword(s):  
High Precision ◽  
High Throughput ◽  
Structure Determination ◽  
Protein Crystallography

Molecular replacement and high-throughput structure determination

2002 ◽  
Vol 217 (12) ◽  
Author(s):  
J. Navaza ◽  
P. M. Alzari
Keyword(s):  
High Throughput ◽  
Structure Determination ◽  
Solution Space ◽  
Molecular Replacement ◽  
Fast Rotation ◽  
The Way

AbstractWe describe the philosophy of the MR method as implemented in the AMoRe package. Fast rotation and translation functions are first used to obtain a meaningful sampling of solution space, whose elements are subsequently assessed by using more robust criteria. The introduction of fast and accurate algorithms for screening a large number of possible solutions opened the way to automation, thus bringing MR methods to the realm of high-throughput structure determination. Selected examples are discussed to illustrate specific aspects of the method.

High-speed and high-precision fluorescence-based cell count and viability assays using the Cellaca™ MX high-throughput cell counter

Cytotherapy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. S97
Author(s):  
J. Bell ◽  
Y. Huang ◽  
S. Yung ◽  
H. Qazi ◽  
C. Hernandez ◽  
...  
Keyword(s):  
Cell Count ◽  
High Precision ◽  
High Throughput ◽  
High Speed ◽  
Cell Counter

scholarly journals Parallel, linear, and subnanometric 3D tracking of microparticles with Stereo Darkfield Interferometry

2021 ◽  
Vol 7 (6) ◽  
pp. eabe3902
Author(s):  
Martin Rieu ◽  
Thibault Vieille ◽  
Gaël Radou ◽  
Raphaël Jeanneret ◽  
Nadia Ruiz-Gutierrez ◽  
...  
Keyword(s):  
High Precision ◽  
High Throughput ◽  
Single Molecule ◽  
Particle Tracking ◽  
Focal Plane ◽  
Tracking System ◽  
Three Dimensional ◽  
3D Tracking ◽  
Single Molecule Force Spectroscopy ◽  
A New Technique

While crucial for force spectroscopists and microbiologists, three-dimensional (3D) particle tracking suffers from either poor precision, complex calibration, or the need of expensive hardware, preventing its massive adoption. We introduce a new technique, based on a simple piece of cardboard inserted in the objective focal plane, that enables simple 3D tracking of dilute microparticles while offering subnanometer frame-to-frame precision in all directions. Its linearity alleviates calibration procedures, while the interferometric pattern enhances precision. We illustrate its utility in single-molecule force spectroscopy and single-algae motility analysis. As with any technique based on back focal plane engineering, it may be directly embedded in a commercial objective, providing a means to convert any preexisting optical setup in a 3D tracking system. Thanks to its precision, its simplicity, and its versatility, we envision that the technique has the potential to enhance the spreading of high-precision and high-throughput 3D tracking.

scholarly journals Novel Electrochemiluminescent Assays for Drug Discovery

2000 ◽  
Vol 5 (1) ◽  
pp. 45-48 ◽  
Author(s):  
Maura C. Kibbey ◽  
David MacAllan ◽  
James W. Karaszkiewicz
Keyword(s):  
Drug Discovery ◽  
High Precision ◽  
High Throughput ◽  
High Performance ◽  
Dynamic Range ◽  
Wide Dynamic Range ◽  
Receptor Ligand ◽  
Enzymatic Assays ◽  
Detection Systems ◽  
Protein Assays

IGEN's ORIGEN® technology, which is based on electrochemiluminescence, has been adopted by a number of research and bioanalytical laboratories who have recognized its exquisite sensitivity, high precision, wide dynamic range, and flexibility in formatting a wide variety of applications. IGEN's M-SERIES™ marks the introduction of the second generation of detection systems employing the ORIGEN technology specifically repackaged to address the needs of the high throughput laboratories involved in drug discovery. Assays are formatted without wash steps. Users realize the high performance of a heterogeneous technology with the convenience of a homogeneous format. The M-SERIES platform can address enzymatic assays (kinases, proteases, helicases, etc.), receptor-ligand or protein-protein assays, immunoassays, quantitation of nucleic acids, as well as other applications. Recent assay formats will be explored in detail.

scholarly journals Toolkit for Multi-Conformation Biomolecular Structure Determination by High-Precision FRET and Molecular Simulations

2015 ◽  
Vol 108 (2) ◽  
pp. 163a-164a
Author(s):  
Mykola Dimura ◽  
Stanislav Kalinin ◽  
Thomas Peulen ◽  
Holger Gohlke ◽  
Claus A.M. Seidel
Keyword(s):  
High Precision ◽  
Structure Determination ◽  
Molecular Simulations ◽  
Biomolecular Structure

RIKEN structural genomics beamlines at the SPring-8; high throughput protein crystallography with automated beamline operation

2006 ◽  
Vol 7 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Go Ueno ◽  
Hiroyuki Kanda ◽  
Raita Hirose ◽  
Koh Ida ◽  
Takashi Kumasaka ◽  
...  
Keyword(s):  
High Throughput ◽  
Structural Genomics ◽  
Protein Crystallography

scholarly journals Micro-array chip for high throughput protein crystallography

2002 ◽  
Vol 58 (s1) ◽  
pp. c94-c94 ◽  
Author(s):  
N. Watanabe ◽  
T. Akita ◽  
T. Sumi ◽  
H. Takeuchi ◽  
I. Tanaka
Keyword(s):  
High Throughput ◽  
Protein Crystallography ◽  
Micro Array
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