scholarly journals Micro electrode arrays for observing spatio-temporal electrical activity pattern in neuronal networks

2000 ◽  
Vol 40 (supplement) ◽  
pp. S2
Author(s):  
A. Kawana
Keyword(s):  
Electrical Activity ◽  
Activity Pattern ◽  
Neuronal Networks ◽  
Electrode Arrays ◽  
Spatio Temporal ◽  
Micro Electrode Arrays

scholarly journals Deuterated Glutamate-Mediated Neuronal Activity on Micro-Electrode Arrays

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 830
Author(s):  
Wataru Minoshima ◽  
Kyoko Masui ◽  
Tomomi Tani ◽  
Yasunori Nawa ◽  
Satoshi Fujita ◽  
...  
Keyword(s):  
Spontaneous Activity ◽  
Neuronal Activity ◽  
Hippocampal Neurons ◽  
Neuronal Networks ◽  
Microelectrode Array ◽  
Mammalian Brain ◽  
Electrode Arrays ◽  
Physiological Properties ◽  
Micro Electrode Arrays

The excitatory synaptic transmission is mediated by glutamate (GLU) in neuronal networks of the mammalian brain. In addition to the synaptic GLU, extra-synaptic GLU is known to modulate the neuronal activity. In neuronal networks, GLU uptake is an important role of neurons and glial cells for lowering the concentration of extracellular GLU and to avoid the excitotoxicity. Monitoring the spatial distribution of intracellular GLU is important to study the uptake of GLU, but the approach has been hampered by the absence of appropriate GLU analogs that report the localization of GLU. Deuterium-labeled glutamate (GLU-D) is a promising tracer for monitoring the intracellular concentration of glutamate, but physiological properties of GLU-D have not been studied. Here we study the effects of extracellular GLU-D for the neuronal activity by using primary cultured rat hippocampal neurons that form neuronal networks on microelectrode array. The frequency of firing in the spontaneous activity of neurons increased with the increasing concentration of extracellular GLU-D. The frequency of synchronized burst activity in neurons increased similarly as we observed in the spontaneous activity. These changes of the neuronal activity with extracellular GLU-D were suppressed by antagonists of glutamate receptors. These results suggest that GLU-D can be used as an analog of GLU with equivalent effects for facilitating the neuronal activity. We anticipate GLU-D developing as a promising analog of GLU for studying the dynamics of glutamate during neuronal activity.

scholarly journals Human neuronal networks on micro-electrode arrays are a highly robust tool to study disease-specific genotype-phenotype correlations in vitro

2021 ◽  
Author(s):  
B. Mossink ◽  
A.H.A. Verboven ◽  
E.J.H. van Hugte ◽  
T.M. Klein Gunnewiek ◽  
G. Parodi ◽  
...  
Keyword(s):  
Experimental Design ◽  
Neuronal Network ◽  
Neuronal Networks ◽  
Network Activity ◽  
Full Potential ◽  
Electrode Arrays ◽  
Neuronal Network Activity ◽  
Micro Electrode Arrays ◽  
Disease Specific

AbstractMicro-electrode arrays (MEAs) are increasingly used to characterize neuronal network activity of human induced pluripotent stem-cell (hiPSC)-derived neurons. Despite their gain in popularity, MEA recordings from hiPSC-derived neuronal networks are not always used to their full potential in respect to experimental design, execution and data analysis. Therefore, we benchmarked the robustness and sensitivity of MEA-derived neuronal activity patterns derived from ten healthy individual control lines. We provide recommendations on experimental design and analysis to achieve standardization. With such standardization, MEAs can be used as a reliable platform to distinguish (disease-specific) network phenotypes. In conclusion, we show that MEAs are a powerful and robust tool to uncover functional neuronal network phenotypes from hiPSC-derived neuronal networks, and provide an important resource to advance the hiPSC field towards the use of MEAs for disease-phenotyping and drug discovery.

scholarly journals Human neuronal networks on micro-electrode arrays are a highly robust tool to study disease-specific genotype-phenotype correlations in vitro

2021 ◽  
Author(s):  
Britt Mossink ◽  
Anouk H.A. Verboven ◽  
Eline J.H. van Hugte ◽  
Teun M. Klein Gunnewiek ◽  
Giulia Parodi ◽  
...  
Keyword(s):  
Neuronal Networks ◽  
Electrode Arrays ◽  
Micro Electrode Arrays ◽  
Disease Specific
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