Multi-Site Optical Recording of Action Potentials from Aplysia Neuronal Network and Analysis of Learning Mechanims

1991 ◽  
Vol 31 (5) ◽  
pp. 3-11
Author(s):  
Satoshi Yamada ◽  
Michio Nakashima ◽  
Satoru Siono
Keyword(s):  
Neuronal Network ◽  
Action Potentials ◽  
Optical Recording

Two-Photon Excitation of Potentiometric Probes Enables Optical Recording of Action Potentials From Mammalian Nerve Terminals In Situ

2008 ◽  
Vol 99 (3) ◽  
pp. 1545-1553 ◽  
Author(s):  
Jonathan A. N. Fisher ◽  
Jonathan R. Barchi ◽  
Cristin G. Welle ◽  
Gi-Ho Kim ◽  
Paul Kosterin ◽  
...  
Keyword(s):  
Action Potentials ◽  
Optical Recording ◽  
Photon Absorption ◽  
Nerve Terminals ◽  
Electrode Arrays ◽  
Two Photon ◽  
Photon Excitation ◽  
Mammalian Nerve ◽  
Two Photon Excitation

We report the first optical recordings of action potentials, in single trials, from one or a few (∼1–2 μm) mammalian nerve terminals in an intact in vitro preparation, the mouse neurohypophysis. The measurements used two-photon excitation along the “blue” edge of the two-photon absorption spectrum of di-3-ANEPPDHQ (a fluorescent voltage-sensitive naphthyl styryl-pyridinium dye), and epifluorescence detection, a configuration that is critical for noninvasive recording of electrical activity from intact brains. Single-trial recordings of action potentials exhibited signal-to-noise ratios of ∼5:1 and fractional fluorescence changes of up to ∼10%. This method, by virtue of its optical sectioning capability, deep tissue penetration, and efficient epifluorescence detection, offers clear advantages over linear, as well as other nonlinear optical techniques used to monitor voltage changes in localized neuronal regions, and provides an alternative to invasive electrode arrays for studying neuronal systems in vivo.

Optical recording of action potentials from vertebrate nerve terminals using potentiometric probes provides evidence for sodium and calcium components

Nature ◽  
1983 ◽  
Vol 306 (5938) ◽  
pp. 36-40 ◽  
Author(s):  
B. M. Salzberg ◽  
A. L. Obaid ◽  
D. M. Senseman ◽  
H. Gainer
Keyword(s):  
Action Potentials ◽  
Optical Recording ◽  
Nerve Terminals

Optical Recording of Action Potentials and Other Discrete Physiological Events: A Perspective from Signal Detection Theory

Physiology ◽  
2007 ◽  
Vol 22 (1) ◽  
pp. 47-55 ◽  
Author(s):  
Lucas Sjulson ◽  
Gero Miesenböck
Keyword(s):  
Action Potential ◽  
Signal Detection ◽  
Action Potentials ◽  
Signal Detection Theory ◽  
Biological Function ◽  
Optical Recording ◽  
Trial Data ◽  
Single Trial ◽  
Optical Reporters ◽  
Potential Recording

Optical imaging of physiological events in real time can yield insights into biological function that would be difficult to obtain by other experimental means. However, the detection of all-or-none events, such as action potentials or vesicle fusion events, in noisy single-trial data often requires a careful balance of tradeoffs. The analysis of such experiments, as well as the design of optical reporters and instrumentation for them, is aided by an understanding of the principles of signal detection. This review illustrates these principles, using as an example action potential recording with optical voltage reporters.

Biomodeling System - Interaction Between Living Neuronal Networks and the Outer World

2007 ◽  
Vol 19 (5) ◽  
pp. 592-600 ◽  
Author(s):  
Suguru N. Kudoh ◽  
◽  
Chie Hosokawa ◽  
Ai Kiyohara ◽  
Takahisa Taguchi ◽  
...  
Keyword(s):  
Information Processing ◽  
Neuronal Network ◽  
Action Potentials ◽  
Hippocampal Neurons ◽  
Neuronal Networks ◽  
Biological Information ◽  
Culture Dish ◽  
Outer World ◽  
Intelligent Information ◽  
Instinctive Behavior

Rat hippocampal neurons reorganized into complex networks in a culture dish with 64 planar microelectrodes and the electrical activity of neurons were recorded from individual sites. Multi-site recording system for extracellular action potentials was used for recording the activity of living neuronal networks and for applying input from the outer world to the network. The living neuronal network was able to distinguish among patterns of evoked action potentials based on different input, suggesting that the living neuronal network can express several pattern independently, meaning that it has fundamental mechanisms for intelligent information processing. We are developing a “biomodeling system,” in which a living neuronal network is connected to a moving robot with premised control rules corresponding to a genetically provided interface of neuronal networks to peripheral systems. Premised rules are described in fuzzy logic and the robot can generate instinctive behavior, avoiding collision. Sensor input from the robot body was sent to a neuronal network, and the robot moved based on commands from the living neuronal network. This is a good modeling system to analyze interaction between biological information processing and electrical devices.

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