Characterization of ascites- and tumor-infiltrating γδ T cells reveals distinct repertoires and a beneficial role in ovarian cancer

immuneACCESS ◽  
2021 ◽  
Author(s):  
E Foord ◽  
LCM Arruda ◽  
A Gaballa ◽  
C Klynning ◽  
M Uhlin
Keyword(s):  
Ovarian Cancer ◽  
T Cells ◽  
Γδ T Cells ◽  
Beneficial Role

scholarly journals Pitfalls in the characterization of circulating and tissue‐resident human γδ T cells

2020 ◽  
Vol 107 (6) ◽  
pp. 1097-1105 ◽  
Author(s):  
Nicola Beucke ◽  
Daniela Wesch ◽  
Hans‐Heinrich Oberg ◽  
Christian Peters ◽  
Jonas Bochem ◽  
...  
Keyword(s):  
T Cells ◽  
Γδ T Cells

Phenotypic characterization of γδ T cells mobilized in response to acute psychological stress

2010 ◽  
Vol 24 (4) ◽  
pp. 608-614 ◽  
Author(s):  
Leila H. Anane ◽  
Kate M. Edwards ◽  
Victoria E. Burns ◽  
Jet J.C.S. Veldhuijzen van Zanten ◽  
Mark T. Drayson ◽  
...  
Keyword(s):  
T Cells ◽  
Psychological Stress ◽  
Γδ T Cells ◽  
Phenotypic Characterization ◽  
Acute Psychological Stress

scholarly journals Ex Vivo Expanded Human Vγ9Vδ2 T-Cells Can Suppress Epithelial Ovarian Cancer Cell Growth

2019 ◽  
Vol 20 (5) ◽  
pp. 1139 ◽  
Author(s):  
Tsui Mao ◽  
Carol Miao ◽  
Yi Liao ◽  
Ying Chen ◽  
Chia Yeh ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
T Cells ◽  
Mononuclear Cells ◽  
Ex Vivo ◽  
Γδ T Cells ◽  
Ovarian Cancer Cells ◽  
Cytotoxic Activities ◽  
Antitumor Effects ◽  
Peripheral Blood Mononuclear

γδ-T-cells have attracted attention because of their potent cytotoxicity towards tumors. Most γδ-T-cells become activated via a major histocompatibility complex (MHC)-independent pathway by the interaction of their receptor, Natural Killer Group 2 Member D (NKG2D) with the tumor-specific NKG2D ligands, including MHC class I-related chain A/B (MICA/B) and UL16-binding proteins (ULBPs), to kill tumor cells. However, despite their potent antitumor effects, the treatment protocols specifically targeting ovarian tumors require further improvements. Ovarian cancer is one of the most lethal and challenging female malignancies worldwide because of delayed diagnoses and resistance to traditional chemotherapy. In this study, we successfully enriched and expanded γδ-T-cells up to ~78% from peripheral blood mononuclear cells (PBMCs) with mostly the Vγ9Vδ2-T-cell subtype in the circulation. We showed that expanded γδ-T-cells alone exerted significant cytotoxic activities towards specific epithelial-type OVCAR3 and HTB75 cells, whereas the combination of γδ-T cells and pamidronate (PAM), a kind of aminobisphosphonates (NBPs), showed significantly enhanced cytotoxic activities towards all types of ovarian cancer cells in vitro. Furthermore, in tumor xenografts of immunodeficient NSG mice, γδ-T-cells not only suppressed tumor growth but also completely eradicated preexisting tumors with an initial size of ~5 mm. Thus, we concluded that γδ-T-cells alone possess dramatic cytotoxic activities towards epithelial ovarian cancers both in vitro and in vivo. These results strongly support the potential of clinical immunotherapeutic application of γδ-T-cells to treat this serious female malignancy.

Identification and characterization of Foxp3+ γδ T cells in mouse and human

2009 ◽  
Vol 125 (2) ◽  
pp. 105-113 ◽  
Author(s):  
Ning Kang ◽  
Long Tang ◽  
Xiaoyan Li ◽  
Dan Wu ◽  
Wenjing Li ◽  
...  
Keyword(s):  
T Cells ◽  
Γδ T Cells ◽  
Identification And Characterization

scholarly journals Characterization of Tumor Reactivity of Human Vγ9Vδ2 γδ T Cells In Vitro and in SCID Mice In Vivo

2004 ◽  
Vol 173 (11) ◽  
pp. 6767-6776 ◽  
Author(s):  
Dieter Kabelitz ◽  
Daniela Wesch ◽  
Elke Pitters ◽  
Margot Zöller
Keyword(s):  
T Cells ◽  
Γδ T Cells ◽  
Scid Mice

Characterization of a Distinct Subpopulation of Bovine γδ T cells

1995 ◽  
Vol 42 (1-10) ◽  
pp. 162-174 ◽  
Author(s):  
M. Amadori ◽  
I. L. Archetti ◽  
R. Verardi ◽  
C. Berneri
Keyword(s):  
T Cells ◽  
Γδ T Cells ◽  
Distinct Subpopulation

scholarly journals Identification and characterization of latency-associated peptide-expressing γδ T cells

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Rafael M. Rezende ◽  
Andre P. da Cunha ◽  
Chantal Kuhn ◽  
Stephen Rubino ◽  
Hanane M’Hamdi ◽  
...  
Keyword(s):  
T Cells ◽  
Γδ T Cells ◽  
Identification And Characterization
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