High-Throughput Sequencing of the B Cell Receptor in African Burkitt Lymphoma Reveals Clues to Pathogenesis

immuneACCESS ◽  
2018 ◽  
Author(s):  
K Lombardo ◽  
D Coffey ◽  
A Morales ◽  
C Carlson ◽  
A Towlerton ◽  
...  
Keyword(s):  
B Cell ◽  
High Throughput ◽  
Burkitt Lymphoma ◽  
High Throughput Sequencing ◽  
Cell Receptor ◽  
B Cell Receptor

scholarly journals High-throughput sequencing of the B-cell receptor in African Burkitt lymphoma reveals clues to pathogenesis

2017 ◽  
Vol 1 (9) ◽  
pp. 535-544 ◽  
Author(s):  
Katharine A. Lombardo ◽  
David G. Coffey ◽  
Alicia J. Morales ◽  
Christopher S. Carlson ◽  
Andrea M. H. Towlerton ◽  
...  
Keyword(s):  
B Cell ◽  
High Throughput ◽  
Burkitt Lymphoma ◽  
High Throughput Sequencing ◽  
Cell Receptor ◽  
B Cell Receptor ◽  
Key Points ◽  
Mutational Processes

Key Points High-throughput sequencing of primary African Burkitt lymphoma tumors suggests disrupted immunoglobulin rearrangements in BL progenitors. Extensive mutation of expressed and nonexpressed IGH rearrangements suggests multiple active mutational processes in BL tumors.

scholarly journals Complete T and B cell receptor repertoire analysis in rheumatoid arthritis using high throughput sequencing

2010 ◽  
Vol 69 (Suppl 2) ◽  
pp. A33-A34
Author(s):  
P. L. Klarenbeek ◽  
M. E. Doorenspleet ◽  
B. D. C. van Schaik ◽  
M. M. Herenius ◽  
M. E. Jakobs ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
B Cell ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Cell Receptor ◽  
B Cell Receptor ◽  
Receptor Repertoire ◽  
Repertoire Analysis ◽  
Cell Receptor Repertoire

scholarly journals Signatures of B-cell receptor diversity in B lymphocytes following Epstein-Barr virus transformation

2019 ◽  
Vol 51 (6) ◽  
pp. 197-207
Author(s):  
Meimei Lai ◽  
Qiongdan Wang ◽  
Yutian Lu ◽  
Xi Xu ◽  
Ying Xia ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Epstein Barr Virus ◽  
High Throughput Sequencing ◽  
Cell Receptor ◽  
B Cell Receptor ◽  
Human Virus ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr

Epstein-Barr virus (EBV) is a widespread human virus that establishes latent infection, potentially leading to tumors, hematological disorders, and other severe diseases. EBV infections are associated with diverse symptoms and affect various organs; therefore, early diagnosis and treatment are crucial. B cell receptor (BCR) repertoires of B cell surface immunoglobulins have been widely studied for their association with various infectious diseases. However, the specific genetic changes that modulate the BCR repertoires after an EBV infection are still poorly understood. In this study, we employed high-throughput sequencing (HTS) to investigate the diversity of BCR repertoires in an EBV-transformed lymphoblastic cell line (LCL). Compared with the noninfected control B cell line, the LCL exhibited a decrease in overall BCR diversity but displayed an increase in the expansion of some dominant rearrangements such as IGHV4-31/IGHJ4, IGHV4-59/IGHJ4, IGHV5-51/IGHJ3, and IGHV3-74/IGHJ3. A higher frequency of occurrence of these rearrangement types was confirmed in patients with EBV infection. Interestingly, the IGHV3-74 rearrangement was only detected in EBV-infected children, suggesting that our experimental observations were not coincidental. In addition, we identified a highly dominant consensus motif, CAR(xRx)YGSG(xYx)FD, in complementarity-determining region 3 (CDR3) sequences of the heavy chain in the LCL. Our findings demonstrated the utility of HTS technology for studying the variations in signature motifs of the BCR repertoires after EBV infection. We propose that the analysis of BCR repertoire sequences represents a promising method for diagnosing early EBV infections and developing novel antibody- and vaccine-based therapies against such infections.

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