Genotypic Frequency in Asian Native Chicken Populations and Gene Expression Using Insulin-like Growth Factor 1 (IGF1) Gene Promoter Polymorphism

2009 ◽  
Vol 46 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Hla Hla Moe ◽  
Takeshi Shimogiri ◽  
Kotaro Kawabe ◽  
Masahide Nishibori ◽  
Shin Okamoto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Gene Promoter ◽  
Promoter Polymorphism ◽  
Genotypic Frequency ◽  
Insulin Like Growth Factor ◽  
Native Chicken ◽  
Gene Promoter Polymorphism

Antagonistic effects of phorbol esters on insulin regulation of insulin-like growth factor-binding protein-1 (IGFBP-1) but not glucose-6-phosphatase gene expression

2001 ◽  
Vol 359 (3) ◽  
pp. 611-619 ◽  
Author(s):  
Satish PATEL ◽  
Pamela A. LOCHHEAD ◽  
Graham RENA ◽  
Calum SUTHERLAND
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Map Kinases ◽  
Phorbol Esters ◽  
Binding Protein ◽  
Gene Promoter ◽  
Dependent Manner ◽  
Insulin Like Growth Factor ◽  
Factor Binding

Glucose-6-phosphatase (G6Pase) and insulin-like growth factor-binding protein-1 (IGFBP-1) genes contain a homologous promoter sequence that is required for gene repression by insulin. Interestingly, this element interacts with members of the forkhead family of transcription factors [e.g. HNF3 (hepatic nuclear factor 3), FKHR (forkhead in rhabdomyosarcoma)] in vitro, while insulin promotes the phosphorylation and inactivation of FKHR in a phosphatidylinositol 3-kinase- and protein kinase B (PKB)-dependent manner. This mechanism has been proposed to underlie insulin action on G6Pase and IGFBP-1 gene transcription. However, we find that treatment of cells with phorbol esters mimics the effect of insulin on G6Pase, but not IGFBP-1, gene expression. Indeed, phorbol ester treatment actually blocks the ability of insulin to repress IGFBP-1 gene expression. In addition, the action of phorbol esters is significantly reduced by inhibition of the p42/p44 mitogen-activated protein (MAP) kinase pathway. However insulin-induced phosphorylation of PKB or FKHR is not affected by the presence of phorbol esters. Therefore we suggest that activation of p42/p44 MAP kinases will reduce the sensitivity of the IGFBP-1 gene promoter, but not the G6Pase gene promoter, to insulin. Importantly, the activation of PKB and phosphorylation of FKHR is not, in itself, sufficient to reduce IGFBP-1 gene expression in the presence of phorbol esters.

scholarly journals Transcriptional Activation of Insulin-Like Growth Factor-Binding Protein-4 by 17β-Estradiol in MCF-7 Cells: Role of Estrogen Receptor-Sp1 Complexes*

Endocrinology ◽  
1999 ◽  
Vol 140 (6) ◽  
pp. 2501-2508 ◽  
Author(s):  
Chunhua Qin ◽  
Pomila Singh ◽  
Stephen Safe
Keyword(s):  
Gene Expression ◽  
Estrogen Receptor ◽  
Growth Factor ◽  
Binding Protein ◽  
Gene Promoter ◽  
Insulin Like Growth Factor ◽  
Factor Binding ◽  
Human Estrogen Receptor ◽  
17Β Estradiol ◽  
Mcf 7

Abstract Insulin-like growth factor-binding protein-4 (IGFBP-4) is expressed in MCF-7 human breast cancer cells, and treatment of these cells with 17β-estradiol (E2) resulted in induction of IGFBP-4 gene expression (>3-fold) and protein secretion (>6-fold). To identify genomic sequences associated with E2 responsiveness, the 5′-promoter region (−1214 to +18) of the IGFBP-4 gene was cloned into a vector upstream from the firefly luciferase reporter gene, and E2 induced a 10-fold increase in luciferase activity in MCF-7 cells transiently transfected with this construct. Deletion analysis of this region of the IGFBP-4 gene promoter identified two GC-rich sequences at −559 to −553 and −72 to −64 that were important for E2-induced trans-activation. Gel mobility shift assays using 32P-labeled −569 to −540 and −83 to −54 oligonucleotides from the IGFBP-4 gene promoter showed that Sp1 protein bound these oligonucleotides to form a retarded band, and the intensity of the band was competitively decreased after coincubation with unlabeled IGFBP-4-derived and consensus Sp1 oligonucleotides. Mutation of the GC-rich sites within these sequences resulted in loss of the retarded band formation. Wild-type human estrogen receptor did not bind directly to the IGFBP-4 oligonucleotides; however, human estrogen receptor enhanced Sp1-DNA binding in a concentration-dependent manner. The results of this study demonstrate that at least two GC-rich sequences at −559 to −553 and− 72 to −64 are required for induction of IGFBP-4 gene expression by E2 in MCF-7 cells.

scholarly journals Association between an Insulin-Like Growth Factor I Gene Promoter Polymorphism and Bone Mineral Density in the Elderly: The Rotterdam Study

2003 ◽  
Vol 88 (8) ◽  
pp. 3878-3884 ◽  
Author(s):  
Fernando Rivadeneira ◽  
Jeanine J. Houwing-Duistermaat ◽  
Norbert Vaessen ◽  
Jeannette M. Vergeer-Drop ◽  
Albert Hofman ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Growth Factor ◽  
Gene Promoter ◽  
The Elderly ◽  
Promoter Polymorphism ◽  
Rotterdam Study ◽  
Mineral Density ◽  
Factor I ◽  
I Gene ◽  
Gene Promoter Polymorphism

The TF-603A/G gene promoter polymorphism and circulating monocyte tissue factor gene expression in healthy volunteers

2004 ◽  
Vol 91 (02) ◽  
pp. 248-254 ◽  
Author(s):  
Jean-Luc Reny ◽  
Ingrid Laurendeau ◽  
Pierre Fontana ◽  
Ivan Bièche ◽  
Annabelle Dupont ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tissue Factor ◽  
Ex Vivo ◽  
Gene Promoter ◽  
Promoter Polymorphism ◽  
Insertion Polymorphism ◽  
Mrna Levels ◽  
Tf Gene ◽  
Over Time ◽  
Gene Promoter Polymorphism

SummaryThree single nucleotide polymorphisms (-603A/G, -1322C/T, -1812C/T) and one deletion/insertion polymorphism (-1208D/I) are present in the tissue factor (TF) gene promoter sequence. These polymorphisms are in complete linkage disequilibrium, determining two haplotypes with almost equal frequency. The -603A/-1208D/-1322C/-1812C haplotype, presently defined as TF-603A, has been linked to venous thromboembolic disease, with a potentially protecting effect. The effects of the TF-603A/G gene polymorphism on monocyte gene expression and on a whole-blood clotting time (WBCT) are not known. We determined the WBCT in basal conditions (H0) and after 4 hours of LPS stimulation ex vivo (H4LPS) on blood samples from 100 young healthy caucasian male subjects on 2 visits, 7 days apart. Monocyte TF mRNA was quantified at H0 and H4LPS by real-time quantitative reverse-transcription PCR. The monocyte TF mRNA values determined at the first and second visits were concordant. In H4LPS samples, TF mRNA levels were increased 70-fold. The TF-603A haplotype was associated with 40%-lower TF mRNA levels at H0 (P=0.0002) and this association followed the same trend but was no longer significant at H4LPS. At H4LPS, TF mRNA levels were associated with WBCT shortening (P=0.0003). WBCT at H0 was not concordant over time, precluding any genotype-phenotype analysis. WBCT at H4LPS was concordant over time but was not related to the TF-603A/G polymorphism. The TF-603A/G gene promoter polymorphism thus significantly influences constitutive TF gene expression in human monocytes but has no major effect on TF gene expression or on WBCT in LPS stimulated conditions.

scholarly journals The Influence of an Insulin-Like Growth Factor I Gene Promoter Polymorphism on Hip Bone Geometry and the Risk of Nonvertebral Fracture in the Elderly: The Rotterdam Study

2004 ◽  
Vol 19 (8) ◽  
pp. 1280-1290 ◽  
Author(s):  
Fernando Rivadeneira ◽  
Jeanine J Houwing-Duistermaat ◽  
Thomas J Beck ◽  
Joop AMJL Janssen ◽  
Albert Hofman ◽  
...  
Keyword(s):  
Growth Factor ◽  
Gene Promoter ◽  
Bone Geometry ◽  
The Elderly ◽  
Promoter Polymorphism ◽  
Nonvertebral Fracture ◽  
Rotterdam Study ◽  
Factor I ◽  
I Gene ◽  
Gene Promoter Polymorphism

132: Association of MMP-1 Gene Promoter Polymorphism with the Risk of Prostate Cancer and MMP-1 Expression in Radical Prostatectomy Specimens

2006 ◽  
Vol 175 (4S) ◽  
pp. 42-42
Author(s):  
Norihiko Tsuchiya ◽  
Shintaro Narita ◽  
Teruaki Kumazawa ◽  
Yohei Horikawa ◽  
Hideaki Kakinuma ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Radical Prostatectomy ◽  
Gene Promoter ◽  
Promoter Polymorphism ◽  
Gene Promoter Polymorphism

The Role of TNFalpha Gene Promoter Polymorphism in the Development of Coal Workers' Pneumoconiosis

2002 ◽  
Vol 14 (2) ◽  
pp. 117
Author(s):  
Byoung Yong Ahn ◽  
Kyoung Ah Kim ◽  
Hae Yun Nam ◽  
Je Hyeok Mun ◽  
Jin Sook Jeoung ◽  
...  
Keyword(s):  
Gene Promoter ◽  
Promoter Polymorphism ◽  
Coal Workers Pneumoconiosis ◽  
Coal Workers ◽  
Gene Promoter Polymorphism
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