scholarly journals Changes in the mRNA Expression of TGFβ Receptor Types II and III in the Anterior Pituitary during Induced Molting in Hens

2004 ◽  
Vol 41 (2) ◽  
pp. 140-146 ◽  
Author(s):  
V.S. Chowdhury ◽  
M. Nishibori ◽  
Y. Yoshimura
Keyword(s):  
Mrna Expression ◽  
Anterior Pituitary ◽  
Tgf Beta ◽  
Beta Receptor

Abstract 017: Hyperglycemia-induced Posttranscriptional Modification Of Proinflammatory Cytokine Mrna Stability Is Mediated Via Hur/PKC-delta Signaling Pathway

2013 ◽  
Vol 113 (suppl_1) ◽  
Author(s):  
Suresh K Verma ◽  
Alexander R Mackie ◽  
Erin E Vaughan ◽  
Tatiana V Abramova ◽  
Raj kishore ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Cell Line ◽  
Signaling Pathway ◽  
Mrna Stability ◽  
Cardiac Fibrosis ◽  
Diabetic Patients ◽  
Tgf Beta ◽  
Intramyocardial Injection ◽  
Pkc Delta ◽  
Increased Risk

Patients with diabetes are predisposed to increased risk of cardiovascular diseases. Persistent interaction of infiltrating macrophages and resident fibroblasts play a critical role in cardiac fibrosis. However, the signaling mechanism is not clear. We hypothesized that macrophage ELAV1 (mRNA stabilizing protein) modulates profibrotic mediators and extracellular matrix turnover by binding to 3′UTR and regulating the mRNA stability of TGF-beta and MMP-9 in hyperglycemic conditions. Mice receiving intramyocardial injection of HuR-specific shRNA showed significant reduction in infarct size and fibrosis area. Reduced fibrosis was associated with decrease in TGF-beta and MMP-9 expression in the myocardium. Conditioned media (CM) from high glucose (HG) treated macrophages significantly increased profibrogenic response (increased mRNA expression of Col1a1, Col3a1 and fibronectin) in fibroblast cell line as compared to fibroblasts incubated with CM from low glucose (LG)-treated macrophages. Knockdown of ELAV1 in HG-treated macrophages abrogated the profibrotic effects in fibroblasts. Indirect immunofluroscence of bone marrow-derived macrophages (BMM) demonstrated that HG increases nuclear ELAV1 export to the cytoplasm. Pharmacological inhibition of Protein kinase C-delta (PKCd) blocked HG-induced ELAV1 nuclear to cytoplasmic translocation. In vitro, stable knockdown of ELAV1 in mouse macrophage cell line RAW 264.7 reduced mRNA expression of TGF-beta and MMP-9 following LPS challenge, accompanied by a marked reduction in the mRNA stability of these genes. Our study here establishes an ELAV1/TGF-beta/MMP-9/PKC-delta signaling axis in the macrophages controlling the profibrogenic responses in fibroblasts, the major contributor in the pathogenesis of fibrosis. Therefore, targeting this signaling pathway might be of therapy value for cardiac fibrosis in diabetic patients.

scholarly journals Expression of TGF-beta receptor 1 and Smads in the tissues of primary spontaneous pneumothorax

2018 ◽  
Vol 10 (3) ◽  
pp. 1765-1774
Author(s):  
Xiaoyu Wang ◽  
Bin You ◽  
Shuo Chen ◽  
Wenqian Zhang ◽  
Bo Tian ◽  
...  
Keyword(s):  
Spontaneous Pneumothorax ◽  
Primary Spontaneous Pneumothorax ◽  
Tgf Beta ◽  
Beta Receptor

scholarly journals Expression of mRNA for IL1 beta, IL6 and TGF beta 1 in developing human bone and cartilage.

1994 ◽  
Vol 42 (6) ◽  
pp. 733-744 ◽  
Author(s):  
R A Dodds ◽  
K Merry ◽  
A Littlewood ◽  
M Gowen
Keyword(s):  
Mrna Expression ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Close Association ◽  
Interleukin 1 ◽  
Cell Types ◽  
Tgf Beta ◽  
Specific Stage ◽  
Growth Factor Beta

Using in situ hybridization, we investigated the expression of mRNA for interleukin-1 beta (IL1 beta), interleukin-6 (IL6), and transforming growth factor-beta-1 (TGF beta 1) in sections of developing bone in human osteophytes. The expression was related to the cellular activity of alkaline phosphatase to aid in the identification of pre-osteoblast populations. IL1 beta mRNA was localized in active osteoblasts within distinct areas of intramembranous ossification. However, the expression was sporadic and appeared to occur at a specific stage of the osteoblast life cycle. There was no IL1 beta mRNA expression in any cell types during endochondral ossification. IL6 mRNA expression was located within pre-osteoblasts and in newly differentiated and matrix-secreting osteoblasts; expression was absent or reduced in flattened, inactive osteoblasts. Weak or no IL6 expression was observed in chondroblasts and chondrocytes, respectively. However, there was a close association between IL6 mRNA expression and the differentiation of mesenchymal cells into osteoblasts. TGF beta 1 expression was localized to osteoblasts apposed to bone or cartilage matrix; the intensity of expression correlated with matrix secretion. Chondroblasts and chondrocytes expressed lower but significant levels of TGF beta 1 mRNA; the expression was lost with the progression to calcifying cartilage. The three cytokines studied were differentially expressed both temporally and spatially, suggesting different roles for each in osteoblast and chondrocyte function.

scholarly journals Control of TGF-beta receptor expression in bone

10.2741/a268 ◽  
1998 ◽  
Vol 3 (4) ◽  
pp. d113-124 ◽  
Author(s):  
Michael Centrella
Keyword(s):  
Receptor Expression ◽  
Tgf Beta ◽  
Beta Receptor
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