scholarly journals Effects of Ultraviolet Irradiation on the Migratory Ability of Primordial Germ Cells (PGCs) in the Domestic Chicken

2004 ◽  
Vol 41 (2) ◽  
pp. 110-119 ◽  
Author(s):  
Takeo Minematsu ◽  
Yukio Kanai ◽  
Atsushi Tajima
Keyword(s):  
Germ Cells ◽  
Ultraviolet Irradiation ◽  
Primordial Germ Cells ◽  
Domestic Chicken

Production of germ line chimera by transfer of primordial germ cells in the domestic chicken ()

1993 ◽  
Vol 40 (3) ◽  
pp. 509-519 ◽  
Author(s):  
A. Tajima ◽  
M. Naito ◽  
Y. Yasuda ◽  
T. Kuwana
Keyword(s):  
Germ Cells ◽  
Germ Line ◽  
Primordial Germ Cells ◽  
Domestic Chicken

scholarly journals Elimination of primordial germ cells in sturgeon embryos by ultraviolet irradiation†

2018 ◽  
Vol 99 (3) ◽  
pp. 556-564 ◽  
Author(s):  
Taiju Saito ◽  
Hilal Güralp ◽  
Viktoriia Iegorova ◽  
Marek Rodina ◽  
Martin Pšenička
Keyword(s):  
Germ Cells ◽  
Ultraviolet Irradiation ◽  
Primordial Germ Cells

Conservation of chicken male germline by orthotopic transplantation of primordial germ cells from genetically distant donors†

2019 ◽  
Vol 101 (1) ◽  
pp. 200-207 ◽  
Author(s):  
Jitka Mucksová ◽  
Markéta Reinišová ◽  
Jiří Kalina ◽  
Barbora Lejčková ◽  
Jiří Hejnar ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Genome Editing ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Avian Species ◽  
Domestic Chicken ◽  
White Leghorn ◽  
Orthotopic Transplantation ◽  
Viable Offspring ◽  
Male Germline

Abstract Successful derivation and cultivation of primordial germ cells (PGCs) opened the way to efficient transgenesis and genome editing in the chicken. Furthermore, implantation of male PGCs from non-chicken galliform species into the chicken embryos resulted in cross-species germline chimeras and viable offspring. We have recently improved the PGC technology by demonstrating that chicken male PGCs transplanted into the testes of adult cockerel recipients mature into functional sperms. However, the availability of this orthotopic transplantation for cross-species transfer remains to be explored. Here we tested the capacity of genetically distant male PGCs to mature in the microenvironment of adult testes. We derived PGCs from the Chinese black-bone Silkie and transplanted them into infertile White Leghorn cockerels. Within 15–18 weeks after transplantation, we observed restoration of spermatogenesis in recipient cockerels and production of healthy progeny derived from the transplanted PGCs. Our findings also indicate the possibility of cross-species orthotopic transplantation of PGCs. Thus, our results might contribute to the preservation of endangered avian species and maintaining the genetic variability of the domestic chicken.

Hexachlorobenzene toxicity in the rat ovary: II. Ultrastructure induced by medium (10 mg/kg) dose exposure

1992 ◽  
Vol 50 (1) ◽  
pp. 668-669
Author(s):  
Amreek Singh ◽  
Warren G. Foster ◽  
Anna Dykeman ◽  
David C. Villeneuve
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Surface Epithelium ◽  
Morphological Parameters ◽  
Comprehensive Investigation ◽  
Ovary Surface Epithelium ◽  
Rat Ovary ◽  
High Doses

Hexachlorobenzene (HCB) is a known toxicant that is found in the environment as a by-product during manufacture of certain pesticides. This chlorinated chemical has been isolated from many tissues including ovary. When administered in high doses, HCB causes degeneration of primordial germ cells and ovary surface epithelium in sub-human primates. A purpose of this experiment was to determine a no-effect dose of the chemical on the rat ovary. The study is part of a comprehensive investigation on the effects of the compound on the biochemical, hematological, and morphological parameters in the monkey and rat.

Transfusion of Chick Primordial Germ Cells into Quail Embryos and their Settlement in Gonads

1998 ◽  
Vol 69 (10) ◽  
pp. 911-915 ◽  
Author(s):  
Tamao ONO ◽  
Ryohei YOKOI ◽  
Seishi MAEDA ◽  
Takao NISHIDA ◽  
Hirohiko AOYAMA
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells
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