scholarly journals cDNA Cloning and mRNA Expression of Estrogen Receptor α in Japanese Quail

2003 ◽  
Vol 40 (2) ◽  
pp. 121-129 ◽  
Author(s):  
Kouhei Ichikawa ◽  
Ichiro Yamamoto ◽  
Akira Tsukada ◽  
Noboru Saito ◽  
Kiyoshi Shimada
Keyword(s):  
Estrogen Receptor ◽  
Mrna Expression ◽  
Cdna Cloning ◽  
Japanese Quail ◽  
Estrogen Receptor Α

scholarly journals Comparative study of estrogen receptor α, β mRNA expressions of endometriosis and normal endometrium in women and analysis of potential synthetic anti-estrogens in silico

2018 ◽  
Vol 91 (2) ◽  
Author(s):  
Eldafira Eldafira ◽  
Abinawanto Abinawanto ◽  
Luthfiralda Sjahfirdi ◽  
Asmarinah Asmarinah ◽  
Purnomo Soeharso ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Mrna Expression ◽  
Estrogen Receptor Α ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Normal Endometrium ◽  
Pcr Method ◽  
Genetic And Environmental Factors

Endometriosis is a multifactorial disease in which genetic and environmental factors interact causing its pathogenesis. The aim of this study was to investigate the expression pattern of estrogen receptor α (ERα) and β (ERβ) in endometriosis patients compared to normal endometrioum (n=18) as a control by using Quantitative Real Time PCR method. Moreover, we also measured serum estradiol levels of endometriosis patients in the proliferation phase of the menstrual cycle using the enzyme-linked immunosorbent assay method. The mRNA expression of ERβ was significantly higher in the endometriosis group compared to control, and the result of t-test showed that were significantly different (P<0.05). Overexpression of ERβ in endometriosis was likely to have other significant important impacts in the pathology of endometriosis that allowed ERβ to stimulate prostaglandin production in endometriosis tissue and cells. Estradiol content did not correlate with the ERα expression, and it is weakly correlated with ERβ mRNA expression. Molecular docking analysis showed that ERα and ERβ have different binding interactions with synthetic antiestrogens, whereas the best inhibitor was Ral2 to ERα and Aco1 to ERβ. Thus, both inhibitors could be used as leads in further investigation of ERα, ERβ inhibitory activities in vitro and in vivo.

scholarly journals Differential Expression of Myometrial Oxytocin Receptor and Prostaglandin H Synthase 2, But Not Estrogen Receptor α and Heat Shock Protein 90 Messenger Ribonucleic Acid in the Gravid Horn and Nongravid Horn in Sheep during Betamethasone-Induced Labor1

Endocrinology ◽  
1999 ◽  
Vol 140 (12) ◽  
pp. 5712-5718 ◽  
Author(s):  
Wen Xuan Wu ◽  
Xiao Hong Ma ◽  
Toshiyuki Yoshizato ◽  
Norio Shinozuka ◽  
Peter W. Nathanielsz
Keyword(s):  
Estrogen Receptor ◽  
Mrna Expression ◽  
Heat Shock Protein 90 ◽  
Late Pregnancy ◽  
Mechanical Stretch ◽  
Estrogen Receptor Α ◽  
Oxytocin Receptor ◽  
Induced Labor ◽  
Prostaglandin H ◽  
Term Labor

Abstract In the present study, we characterized four myometrial contraction-associated proteins (mCAPs): oxytocin receptor (OTR), prostaglandin H synthase 2 (PGHS2), estrogen receptor α (ERα), and heat shock protein 90 (Hsp90) messenger RNA (mRNA) expression in the nongravid horn of pregnant sheep and compared them with their expression in the gravid horn that is exposed to a greater degree of stretch. We also examined the regulatory effects of estrogen and progesterone on OTR mRNA expression in ovariectomized nonpregnant sheep. In addition, we determined the ontogeny of mCAP expression in the gravid horn throughout late pregnancy and during spontaneous term labor. Gravid horn and nongravid horn myometria were removed under general anesthesia from control ewes not in labor at 130–140 days gestational age (dGA; n = 3) and during betamethasone-induced labor (n = 6) at the same gestational age. Gravid horn myometrium was also collected from ewes not in labor at 95 dGA (n = 3), 101–110 dGA (n = 3), 111–120 dGA (n = 3), 121–130 dGA (n = 3), 131–140 dGA (n = 3), and 141–145 dGA (n = 4) and from ewes in spontaneous term labor (n = 4). All ewes were carrying single fetuses. Myometrium was also collected from ovariectomized nonpregnant ewes treated with saline (n = 5), estradiol (50 μg/day; n = 5), progesterone (0.3 g, intravaginally; n = 5), and estradiol plus progesterone (n = 5). Myometrial RNA was extracted and analyzed by Northern blot for OTR, PGHS2, ERα, and Hsp90 mRNA, normalized for 18S ribosomal RNA orβ -actin. ERα, Hsp90, OTR, and PGHS2 mRNA were all significantly up-regulated during betamethasone-induced labor (P&lt; 0.01) in gravid and nongravid horn myometrium. The level of gravid horn OTR mRNA during labor was 3 times the level of nongravid horn OTR mRNA (P &lt; 0.0001). Gravid horn PGHS2 mRNA was also higher than nongravid horn PGHS2 (P &lt; 0.02). In contrast, in spontaneous term labor nongravid horn, ERα and Hsp90 mRNA were similar to gravid horn. Myometrial ERα and Hsp90 mRNA remained unchanged throughout late pregnancy and increased at spontaneous term labor (P &lt; 0.05). In contrast, myometrial OTR increased around 130 dGA (P &lt; 0.01) and further increased at spontaneous term labor (P&lt; 0.02). Progesterone significantly inhibited myometrial OTR mRNA expression in nonpregnant sheep and estradiol antagonized progesterone’s inhibitory effect. Mechanical stretch differentially regulated mCAP mRNA expression in the ovine gravid horn and nongravid horn. Mechanical stretch appears largely responsible for increased OTR mRNA and to a lesser degree PGHS2 mRNA. In addition, endocrine factors may be required for full activation of OTR and PGHS2 mRNA associated with labor. ERα and Hsp90 mRNA are not under the control of uterine stretch in keeping with our previous results, indicating that systemic hormones such as estradiol, are prime regulators for these two mCAP mRNA expression during labor.

Localization of estrogen receptor-α and -βmRNA in brain areas controlling sexual behavior in Japanese quail

2005 ◽  
Vol 66 (2) ◽  
pp. 148-154 ◽  
Author(s):  
Krister Halldin ◽  
Jeanette Axelsson ◽  
Claes Holmgren ◽  
Björn Brunström
Keyword(s):  
Estrogen Receptor ◽  
Sexual Behavior ◽  
Japanese Quail ◽  
Estrogen Receptor Α ◽  
Brain Areas

scholarly journals Both Estrogen Receptor α and β Stimulate Pituitary GH Gene Expression

2014 ◽  
Vol 28 (1) ◽  
pp. 40-52 ◽  
Author(s):  
Dimiter Avtanski ◽  
Horacio J. Novaira ◽  
Sheng Wu ◽  
Christopher J. Romero ◽  
Rhonda Kineman ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Mrna Expression ◽  
Transcriptional Control ◽  
Estrogen Receptor Α ◽  
In Vitro Models ◽  
Mrna Levels ◽  
Ovariectomized Mice ◽  
Serum Gh

Abstract Although sex steroids have been implicated in the control of mammalian growth, their direct effect on GH synthesis is less clear. The aim of this study was to establish whether estradiol (E2) directly affects GH synthesis in somatotrophs. Somatotroph GH3 and MtT/S cells were used as in vitro models. At physiological doses of E2 stimulation, GH mRNA levels were increased and the ER antagonist ICI 182,780 completely abolished this effect. Estrogen receptor (ER) α– and ERβ-selective agonists, propylpyrazole triol (PPT), and 2,3-bis(4-hydroxyphenyl) propionitrile (DPN), respectively, augmented GH mRNA expression and secretion, whereas E2 and PPT, but not DPN increased prolactin (PRL) mRNA levels. E2, PPT, and DPN stimulated expression of the pituitary transcription factor Pou1f1 and increased its binding to the GH promoter. In vivo evidence of E2 effects on GH synthesis was obtained from the generation of the somatotroph-specific ERα knockout (sERα-KO) mouse model. Basal pituitary GH, PRL, POU1F1, and ERα mRNA expression levels were lower in sERα-KO mice compared with those in controls; whereas ERβ mRNA levels remained unchanged. E2 and DPN stimulated pituitary GH mRNA expression and serum GH levels in control and sERα-KO ovariectomized mice; however, serum GH levels were unchanged in PPT-treated ovariectomized sERα-KO mice. In these animal models, PRL mRNA levels increased after either E2 or PPT, but an increase was not seen after DPN treatment. Thus, we propose a mechanism by which estrogen directly regulates somatotroph GH synthesis at a pretranslational level. In contrast to the predominant effect of ERα in the lactotroph, these results support a role for both ERα and ERβ in the transcriptional control of Gh in the somatotroph and illustrate important differences in ER isoform specificity in the anterior pituitary gland.

Zebra finch estrogen receptor cDNA: Cloning and mRNA expression

1996 ◽  
Vol 59 (2) ◽  
pp. 135-145 ◽  
Author(s):  
Erin C. Jacobs ◽  
Arthur P. Arnold ◽  
Anthony T. Campagnoni
Keyword(s):  
Estrogen Receptor ◽  
Mrna Expression ◽  
Cdna Cloning ◽  
Zebra Finch ◽  
Receptor Cdna
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