scholarly journals Effect of Heat Stress and L-Ascorbic Acid-2-Phosphate Magnesium on Plasma and Liver Thiobarbituric Acid Reactive Substances Concentrations, and on Liver Protein Carbonyl Concentration in Chicks.

1997 ◽  
Vol 34 (1) ◽  
pp. 63-66 ◽  
Author(s):  
Yosuke AOYAGI ◽  
Takashi OHNISHI ◽  
Shinobu ITOH ◽  
Tetsuro NAKAYA
Keyword(s):  
Ascorbic Acid ◽  
Heat Stress ◽  
Thiobarbituric Acid ◽  
Protein Carbonyl ◽  
Liver Protein ◽  
Thiobarbituric Acid Reactive Substances ◽  
Phosphate Magnesium

scholarly journals Effect of the Cymbopogon citratus Infusion on the Activity of Acetylcholinesterase Enzyme and on the Redox Profile in Farmers’ Erythrocytes

2017 ◽  
Vol 9 (9) ◽  
pp. 68
Author(s):  
Natacha Cossettin Mori ◽  
Roberta Cattaneo Horn ◽  
Caroline Oliveira ◽  
Gabriela Tassotti Gelatti ◽  
Jonathas Zeni Klafke ◽  
...  
Keyword(s):  
Thiobarbituric Acid ◽  
Acetylcholinesterase Activity ◽  
Protein Carbonyl ◽  
Protein Carbonyls ◽  
Thiobarbituric Acid Reactive Substances ◽  
Cymbopogon Citratus ◽  
Agricultural Modernization ◽  
Rural Workers ◽  
Modernization Process ◽  
Acetylcholinesterase Enzyme

Agrochemicals were more prominent in 1960, marked due to the agricultural modernization process. As a result of this widespread use for food production, there was also an increase in cases of intoxication caused by these agents which made it necessary to search for alternative therapies for agricultural workers. Thus, considering that phytochemical characterization revealed the presence of antioxidants in Cymbopogon citratus extract, the objective of this study was to evaluate the effect of this plant infusion on the enzyme acetylcholinesterase activity (AChE) and on the redox response in farmers’ erythrocytes. These erytrocytes were processed and subjected to treatment with the Cymbopogon citratus infusion (5, 10, 25 and 50 g/L). In these samples the following were determined: the AChE enzyme activity, the levels of thiobarbituric acid reactive substances (TBARS), protein carbonyls (CPs) and reduced glutathione (GSH). In general, it was discovered that the inhibition of AChE activity is negative regarding to the increase of protein carbonyl levels and positive regarding the GSH levels. In addition, Cymbopogon citratus infusions could not even reverse this inhibition or the high levels of TBARS and CPs. On the other hand, levels of GSH were increased by infusions demonstrating the increased antioxidant activity in rural workers’ erythrocytes.

Carotenoids from Malanga (Xanthosoma Sagittifolium) Leaves Protect Cells Against Oxidative Stress in Rats

2005 ◽  
Vol 75 (2) ◽  
pp. 161-168 ◽  
Author(s):  
Arruda ◽  
Souza ◽  
Siqueira
Keyword(s):  
Vitamin A ◽  
Protein Oxidation ◽  
Beta Carotene ◽  
Thiobarbituric Acid ◽  
Protein Carbonyl ◽  
Rat Tissues ◽  
Liver Protein ◽  
Xanthosoma Sagittifolium ◽  
Leaf Powder ◽  
Carotene Beta

The present study investigated the effect of malanga leaf powder and its carotenoids oil extract on oxidative damage in rat tissues. A group of rats received AIN-93G diet devoid of vitamin A (AD) and three other groups were treated with AD diet supplied with synthetic beta-carotene (beta-car) or malanga leaf powder (MP) or malanga carotenoids extract (CE). The diets’ carotenoids bioavailability was determined through carotenoids intake/liver retinol content. Lipid thiobarbituric acid reactive substances (TBARS) and protein (carbonyl) oxidation, and reduced (GSH) and oxidized (GSSG) glutathione concentrations were determined in liver, heart, and brain. Synthetic beta-carotene bioavailability was higher than that of pro-vitamin A carotenoids from MP and CE diets, and no difference was observed between the latter two groups. Liver and heart lipid peroxidation was lower in the beta-car and CE groups than the AD group, while no difference was observed for the MP group. The beta-car, MP, and CE groups showed lower liver protein oxidation than the AD group, and only the CE group had lower heart protein oxidation in relation to AD. The MP group had a lower liver GSSG concentration and higher GSH/GSSG ratio than the AD group, while no difference was observed for heart glutathione concentration among the groups. The results indicate that at physiological levels, beta-car, malanga carotenoids extract, and malanga leaf powder have antioxidant effects in rats.

scholarly journals Effect of turmeric powder (Curcuma longa L.) and ascorbic acid on antioxidant capacity and oxidative status in rabbit burgers after cooking

2016 ◽  
Vol 24 (2) ◽  
pp. 121 ◽  
Author(s):  
S. Mancini ◽  
G. Preziuso ◽  
G. Paci
Keyword(s):  
Ascorbic Acid ◽  
Lipid Oxidation ◽  
Antioxidant Capacity ◽  
Storage Time ◽  
Curcuma Longa ◽  
Thiobarbituric Acid ◽  
Antioxidant Effect ◽  
Thiobarbituric Acid Reactive Substances ◽  
Reducing Ability ◽  
Tbars Values

<p>The aim of this study was to evaluate the effects of turmeric powder and ascorbic acid on lipid oxidation and antioxidant capacity in cooked rabbit burgers. The burgers were derived from 3 different formulations (C, control, with no additives; Tu with 3.5% of turmeric powder and AA with 0.1% of ascorbic acid) and were stored at 4°C for 0 and 7 d and cooked. The lipid oxidation (thiobarbituric acid reactive substances [TBARS]) and antioxidant capacity (2,2-azinobis-[3 ethylbenzothiazoline-6-sulfonic acid] {ABTS}, 1,1-diphenyl-2-pircydrazyl [DPPH] and ferric reducing ability [FRAP]) were evaluated. A significant interaction between storage time and formulation (P&lt;0.001) was observed for DPPH, FRAP and TBARS in cooked burgers. At day 0 and day 7, the DPPH value was higher in Tu and AA compared to C burgers. At day 0, C showed a lower level of FRAP than the Tu and AA burgers. At day 7, the FRAP values tended to decrease but remained significantly higher in Tu and AA compared to C burgers. Lipid oxidation at day 0 in Tu and AA showed lower TBARS values compared to C burgers. The addition of 3.5% turmeric powder in rabbit burgers exerts an antioxidant effect during storage and it seems more effective in controlling lipid oxidation than ascorbic acid after cooking.</p>

scholarly journals Association of Hypoadiponectinemia with Hypoglutathionemia in NAFLD Subjects with and without Type 2 Diabetes

2010 ◽  
Vol 29 (5) ◽  
pp. 213-221 ◽  
Author(s):  
Narasimhan Sandhya ◽  
Kuppan Gokulakrishnan ◽  
Radhakrishnan Ravikumar ◽  
Viswanathan Mohan ◽  
Muthuswamy Balasubramanyam
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
Thiobarbituric Acid ◽  
Normal Glucose Tolerance ◽  
Protein Carbonyl ◽  
Protein Carbonyls ◽  
Diabetic Patients ◽  
Thiobarbituric Acid Reactive Substances ◽  
Type 2 Diabetic Patients

Objective: The aim of this study is to measure the extent of oxidative stress and to see whether it has any correlation to changes in adiponectin levels in NAFLD subjects with and without Type 2 diabetes.Methods: Subjects recruited from the Chennai Urban Rural Epidemiology Study comprise of 1: Normal Glucose Tolerance (NGT) subjects without NAFLD; 2: NGT with NAFLD; 3: Type 2 Diabetic patients [T2DM] without NAFLD and 4: T2DM with NAFLD. Thiobarbituric acid reactive substances (TBARS), protein carbonyl (PCO), glutathione and adiponectin levels were measured by standard methods. Ultrasound of the liver was used to diagnose NAFLD.Results: T2DM subjects with NAFLD had significantly (p< 0.001) higher levels of thiobarbituric acid reactive substances (TBARS) and protein carbonyls (PCO) but lower (p< 0.001) GSH/GSSG ratio and adiponectin levels compared to other three groups. The association of hypoadiponectinemia withNAFLD/Type 2 diabetes was significant even after adjusting for age, gender and BMI, but lost when adjusted for parameters of oxidative stress. While palmitate significantly reduced GSH/GSSG ratio in hepatocytes, addition of exogenous recombinant adiponectin restored the GSH/GSSG ratio comparable to those of untreated cells.Conclusion: There exists an association of hypoglutathionemia and hypoadiponectinemia in subjects with NAFLD and/or T2DM. In addition to the known beneficial effects, out study also exposes the antioxidant nature of adiponectin.

Anoxia-reoxygenation versus ischemia in isolated rat lungs

1997 ◽  
Vol 273 (6) ◽  
pp. L1112-L1117 ◽  
Author(s):  
Guochang Zhao ◽  
Abu B. Al-Mehdi ◽  
Aron B. Fisher
Keyword(s):  
Ischemia Reperfusion ◽  
Thiobarbituric Acid ◽  
Protein Carbonyl ◽  
Oxidase Inhibitor ◽  
Thiobarbituric Acid Reactive Substances ◽  
Protein Carbonyl Content ◽  
Xanthine Oxidase Inhibitor ◽  
Fourfold Increase ◽  
Rat Lungs ◽  
Isolated Rat

Oxidant generation in anoxia-reoxygenation and ischemia-reperfusion was compared in isolated rat lungs. Anoxia-reoxygenation was produced by N2 ventilation followed by O2 ventilation. After anoxia, lung ATP content was decreased by 59%. Oxygenated ischemia was produced by discontinuing perfusion while ventilation with O2 was maintained. With anoxia-reoxygenation, oxidant generation, evaluated by oxidation of dichlorodihydrofluorescein (H2DCF) to fluorescent dichlorofluorescein, increased 3.6-fold, lung thiobarbituric acid reactive substances (TBARS) increased 342%, conjugated dienes increased 285%, and protein carbonyl content increased 46%. Pretreatment of lungs with 100 μM allopurinol inhibited the reoxygenation-mediated increase in lung fluorescence by 75% and TBARS by 69%. Oxygenated ischemia resulted in an approximately eightfold increase in lung H2DCF oxidation and a fourfold increase in TBARS, but allopurinol had no effect. On the other hand, 100 μM diphenyliodonium (DPI) inhibited the ischemia-mediated increase in lung fluorescence by 69% and lung TBARS by 70%, but it had no effect on the increase with anoxia-reoxygenation. Therefore, both ischemia-reperfusion and anoxia-reoxygenation result in oxidant generation by the lung, but a comparison of results with a xanthine oxidase inhibitor (allopurinol) and a flavoprotein inhibitor (DPI) indicate that the pathways for oxidant generation are distinctly different.

Effects of Administration of beta-Carotene, Ascorbic Acid, Persimmons, and Pods on Antioxidative Ability in UV-Irradiated ODS Rats

2005 ◽  
Vol 75 (4) ◽  
pp. 227-234 ◽  
Author(s):  
Hosotani ◽  
Yoshida ◽  
Kitagawa
Keyword(s):  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
Beta Carotene ◽  
Thiobarbituric Acid ◽  
Ultraviolet B ◽  
Thiobarbituric Acid Reactive Substances ◽  
Sod Activity ◽  
Antioxidative Ability

To evaluate the effects of supplementing diets with carotenoid and ascorbic acid (AsA) on the antioxidative ability of Osteogenic Disorder-Shionogi (ODS) rats, we added synthetic b-carotene (bC), AsA, and powders of persimmon (Ka) and pods (Po) containing bC and AsA to the diet and obtained the following results. The urinary 8-hydroxydeoxyguanosine (8-OHdG) concentration was low in the –betaC•AsA and +AsA groups but high in the +betaC. AsA, +Ka, and +Po groups. The thiobarbituric acid-reactive substances (TBARS) in both the liver and skin were higher in the –betaC•AsA group than in the +betaC•AsA group and were low in the +Ka and +Po groups. As antioxidant enzymes, glutathione peroxidase (GSH-Px) activity was high in the +betaC•AsA group, low in the –betaC•AsA group in both the skin and liver, and also high in the + Ka and +Po group in the liver. Superoxide dismutase (SOD) activity was high in the –betaC•AsA group and low in the +betaC•AsA and +Ka groups in both the skin and liver. Catalase (CAT) activity in the liver was low in the –betaC•AsA, + AsA, and +betaC groups and high in the +betaC•AsA and + Po groups. These results confirmed that the administration of betaC, AsA, and persimmons and pods increases antioxidative ability in the skin and liver of ultraviolet-b(UV-B)-irradiated ODS rats.

Conjugated Linoleic Acid Causes a Marked Increase in Liver α-Tocopherol and Liver α-Tocopherol Transfer Protein in C57BL/6 J Mice

2010 ◽  
Vol 80 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Pei-Min Chao ◽  
Wan-Hsuan Chen ◽  
Chun-Huei Liao ◽  
Huey-Mei Shaw
Keyword(s):  
Antioxidant Enzymes ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substances ◽  
Control Groups ◽  
Protein Levels ◽  
Transfer Protein ◽  
And Control

Conjugated linoleic acid (CLA) is a collective term for the positional and geometric isomers of a conjugated diene of linoleic acid (C18:2, n-6). The aims of the present study were to evaluate whether levels of hepatic α-tocopherol, α-tocopherol transfer protein (α-TTP), and antioxidant enzymes in mice were affected by a CLA-supplemented diet. C57BL/6 J mice were divided into the CLA and control groups, which were fed, respectively, a 5 % fat diet with or without 1 g/100 g of CLA (1:1 mixture of cis-9, trans-11 and trans-10, cis-12) for four weeks. α-Tocopherol levels in plasma and liver were significantly higher in the CLA group than in the control group. Liver α-TTP levels were also significantly increased in the CLA group, the α-TTP/β-actin ratio being 2.5-fold higher than that in control mice (p<0.01). Thiobarbituric acid-reactive substances were significantly decreased in the CLA group (p<0.01). There were no significant differences between the two groups in levels of three antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). The accumulation of liver α-tocopherol seen with the CLA diet can be attributed to the antioxidant potential of CLA and the ability of α-TTP induction. The lack of changes in antioxidant enzyme protein levels and the reduced lipid peroxidation in the liver of CLA mice are due to α-tocopherol accumulation.

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