scholarly journals Effective generation and twisted weak positivity of direct images

2019 ◽  
Vol 13 (2) ◽  
pp. 425-454 ◽  
Author(s):  
Yajnaseni Dutta ◽  
Takumi Murayama
Keyword(s):  
Weak Positivity ◽  
Effective Generation

scholarly journals Tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Watchareewan Rodprasert ◽  
Sirirat Nantavisai ◽  
Koranis Pathanachai ◽  
Prasit Pavasant ◽  
Thanaphum Osathanon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Hanging Drop ◽  
Differentiation Potential ◽  
Insulin Producing Cells ◽  
Edmonton Protocol ◽  
Effective Generation ◽  
Hanging Drop Culture ◽  
Peptide Secretion

AbstractThe trend of regenerative therapy for diabetes in human and veterinary practices has conceptually been proven according to the Edmonton protocol and animal models. Establishing an alternative insulin-producing cell (IPC) resource for further clinical application is a challenging task. This study investigated IPC generation from two practical canine mesenchymal stem cells (cMSCs), canine bone marrow-derived MSCs (cBM-MSCs) and canine adipose-derived MSCs (cAD-MSCs). The results illustrated that cBM-MSCs and cAD-MSCs contain distinct pancreatic differentiation potential and require the tailor-made induction protocols. The effective generation of cBM-MSC-derived IPCs needs the integration of genetic and microenvironment manipulation using a hanging-drop culture of PDX1-transfected cBM-MSCs under a three-step pancreatic induction protocol. However, this protocol is resource- and time-consuming. Another study on cAD-MSC-derived IPC generation found that IPC colonies could be obtained by a low attachment culture under the three-step induction protocol. Further, Notch signaling inhibition during pancreatic endoderm/progenitor induction yielded IPC colonies through the trend of glucose-responsive C-peptide secretion. Thus, this study showed that IPCs could be obtained from cBM-MSCs and cAD-MSCs through different induction techniques. Also, further signaling manipulation studies should be conducted to maximize the protocol’s efficiency.

Cost-effective generation of A-to-G mutant mice by zygote electroporation of adenine base editor ribonucleoproteins

2020 ◽  
Vol 47 (6) ◽  
pp. 337-340
Author(s):  
Hongwei Sun ◽  
Shengyao Zhi ◽  
Guifang Wu ◽  
Guanglan Wu ◽  
Tianqi Cao ◽  
...  
Keyword(s):  
Cost Effective ◽  
Mutant Mice ◽  
Effective Generation ◽  
Adenine Base

Theoretical Preconditions of Creation of Self-Adjusted Mechanisms

2016 ◽  
Vol 859 ◽  
pp. 37-45
Author(s):  
Konstantin Samson Ivanov
Keyword(s):  
Control System ◽  
External Loading ◽  
Closed Contour ◽  
Force Analysis ◽  
Proved Theorem ◽  
Transfer Ratio ◽  
Effective Generation ◽  
Highly Effective ◽  
Force Moment

On the basis of the force analysis of two mobile mechanisms containing a mobile closed contour the theoretical preconditions of creation of self-adjusted mechanisms is developed. The load design of mechanism is executed on the basis of the proved theorem of a derivative on a time from the force moment. Brand new property of self-adjustment is found. Mechanism self-adjustment allows it to be independently conforming to variable external loading by change of speed. Property of self-adjustment allows creating the mechanisms with variable transfer ratio depending on loading. Self-adjusted mechanisms are simple on the device and are working without a control system. Self-adjusted mechanisms (for example, self-adjusted gear boxes) are determining a new highly effective generation of drives of machines with variable technological resistance.

Scalable and cost-effective generation of osteogenic micro-tissues through the incorporation of inorganic microparticles within mesenchymal stem cell spheroids

2019 ◽  
Vol 12 (1) ◽  
pp. 015021 ◽  
Author(s):  
Ibrahim Zarkesh ◽  
Majid Halvaei ◽  
Mohammad Hossein Ghanian ◽  
Fatemeh Bagheri ◽  
Forough Azam Sayahpour ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cost Effective ◽  
Cell Spheroids ◽  
Effective Generation
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