SensPIV: Simultaneous Visualization of Flow Fields and Oxygen Concentrations to Unravel Metabolic Exchange Fluxes

2022 ◽  
Author(s):  
Soeren Ahmerkamp ◽  
Farooq M. Jalaluddin ◽  
Yuan Cui ◽  
Douglas R. Brumley ◽  
Cesar O. Pacherres ◽  
...  
Keyword(s):  
Flow Fields ◽  
Simultaneous Visualization ◽  
Metabolic Exchange ◽  
Oxygen Concentrations

Three-Dimensional Subcellular Organization of Hepatocytes in Intact Liver: Simultaneous Visualization of Cytoskeletal and Membrane Markers by Confocal Microscopy

1996 ◽  
Vol 54 ◽  
pp. 288-289
Author(s):  
Greg V. Martin ◽  
Ann L. Hubbard
Keyword(s):  
Three Dimensional ◽  
Integral Membrane Proteins ◽  
Polarized Secretion ◽  
Microscope Images ◽  
Computer Aided ◽  
Intracellular Organelles ◽  
Cytoskeletal Elements ◽  
Simultaneous Visualization

The microtubule (MT) cytoskeleton is necessary for many of the polarized functions of hepatocytes. Among the functions dependent on the MT-based cytoskeleton are polarized secretion of proteins, delivery of endocytosed material to lysosomes, and transcytosis of integral plasma membrane (PM) proteins. Although microtubules have been shown to be crucial to the establishment and maintenance of functional and structural polarization in the hepatocyte, little is known about the architecture of the hepatocyte MT cytoskeleton in vivo, particularly with regard to its relationship to PM domains and membranous organelles. Using an in situ extraction technique that preserves both microtubules and cellular membranes, we have developed a protocol for immunofluorescent co-localization of cytoskeletal elements and integral membrane proteins within 20 µm cryosections of fixed rat liver. Computer-aided 3D reconstruction of multi-spectral confocal microscope images was used to visualize the spatial relationships among the MT cytoskeleton, PM domains and intracellular organelles.

Recurrence of fragmenting granules and their relation to meso- and supergranular flow fields

2003 ◽  
Vol 9 ◽  
pp. 371-371
Author(s):  
Th. Roudier ◽  
F. Lignières ◽  
M. Rieutord ◽  
P. N. Brandt ◽  
J.-M. Malherbe
Keyword(s):  
Flow Fields

Measurement of post-separated flow fields on airfoils

1975 ◽  
Author(s):  
H. SEETHARAM ◽  
W. WENTZ, JR. ◽  
J. WALKER
Keyword(s):  
Separated Flow ◽  
Flow Fields
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