Dendritic Peptide-Conjugated Polymeric Nanovectors for Nontoxic Delivery of Plasmid DNA and Enhanced Non-Viral Transfection of Immune Cells

Novel lipid combination for delivery of plasmid DNA to immune cells in the spleen

Journal of Controlled Release ◽

10.1016/j.jconrel.2021.01.005 ◽

2021 ◽

Author(s):

Seigo Kimura ◽

Ikramy A. Khalil ◽

Yaser H.A. Elewa ◽

Hideyoshi Harashima

Keyword(s):

Immune Cells ◽

Plasmid Dna

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Engineered Nanodelivery Systems to Improve DNA Vaccine Technologies

Pharmaceutics ◽

10.3390/pharmaceutics12010030 ◽

2020 ◽

Vol 12 (1) ◽

pp. 30 ◽

Cited By ~ 12

Author(s):

Michael Lim ◽

Abu Zayed Md Badruddoza ◽

Jannatul Firdous ◽

Mohammad Azad ◽

Adnan Mannan ◽

...

Keyword(s):

Immune Responses ◽

Clinical Application ◽

Dna Vaccine ◽

Immune Cells ◽

Plasmid Dna ◽

Dna Vaccines ◽

Immunological Response ◽

Main Challenge ◽

Human Immunodeficiency Viruses ◽

Stimulation Of

DNA vaccines offer a flexible and versatile platform to treat innumerable diseases due to the ease of manipulating vaccine targets simply by altering the gene sequences encoded in the plasmid DNA delivered. The DNA vaccines elicit potent humoral and cell-mediated responses and provide a promising method for treating rapidly mutating and evasive diseases such as cancer and human immunodeficiency viruses. Although this vaccine technology has been available for decades, there is no DNA vaccine that has been used in bed-side application to date. The main challenge that hinders the progress of DNA vaccines and limits their clinical application is the delivery hurdles to targeted immune cells, which obstructs the stimulation of robust antigen-specific immune responses in humans. In this updated review, we discuss various nanodelivery systems that improve DNA vaccine technologies to enhance the immunological response against target diseases. We also provide possible perspectives on how we can bring this exciting vaccine technology to bedside applications.

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The study of complexation between dicationic surfactants and the DNA duplex using structural and spectroscopic methods

RSC Advances ◽

10.1039/c6ra24978g ◽

2017 ◽

Vol 7 (42) ◽

pp. 26006-26018 ◽

Cited By ~ 7

Author(s):

W. Andrzejewska ◽

M. Wilkowska ◽

M. Chrabąszczewska ◽

M. Kozak

Keyword(s):

Nucleic Acids ◽

Plasmid Dna ◽

Living Cells ◽

Spectroscopic Methods ◽

Dna Duplex ◽

Dna Oligomers ◽

Different Types ◽

Dicationic Surfactants ◽

Viral Transfection

Dicationic (also known as gemini or dimeric) bis-alkylimidazolium surfactants belong to a group of non-viral transfection systems proposed for the successful introduction of different types of nucleic acids (i.e., siRNA, DNA oligomers, and plasmid DNA) into living cells.

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Exhaustivein vivolabelling of plasmid DNA with BrdU for intracellular detection in non-viral transfection of mammalian cells

Biotechnology Journal ◽

10.1002/biot.200900053 ◽

2009 ◽

Vol 4 (10) ◽

pp. 1479-1487 ◽

Cited By ~ 5

Author(s):

Valérie Jérôme ◽

Andreas Heider ◽

Anja Schallon ◽

Ruth Freitag

Keyword(s):

Plasmid Dna ◽

Mammalian Cells ◽

Intracellular Detection ◽

Viral Transfection

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Deciphering the role of substrate stiffness in enhancing the internalization efficiency of plasmid DNA in stem cells using lipid-based nanocarriers

Nanoscale ◽

10.1039/c8nr01516c ◽

2018 ◽

Vol 10 (19) ◽

pp. 8947-8952 ◽

Cited By ~ 8

Author(s):

Saman Modaresi ◽

Settimio Pacelli ◽

Jonathan Whitlow ◽

Arghya Paul

Keyword(s):

Stem Cells ◽

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Plasmid Dna ◽

Substrate Stiffness ◽

Vascular Endothelial ◽

Adipose Derived Stem Cells ◽

Endothelial Growth Factor ◽

Viral Transfection

This study investigates the role of substrate stiffness in the non-viral transfection of human adipose-derived stem cells (hASCs) with the aim to maximize the hASC expression of vascular endothelial growth factor (VEGF).

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