Dipstick-Based Rapid Nucleic Acids Purification and CRISPR/Cas12a-Mediated Isothermal Amplification for Visual Detection of African Swine Fever Virus

2021 ◽  
Author(s):  
Siwenjie Qian ◽  
Yanju Chen ◽  
Cheng Peng ◽  
Xiaofu Wang ◽  
Hui Wu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Visual Detection ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Isothermal Amplification

scholarly journals Field Verification of an African Swine Fever Virus Loop-Mediated Isothermal Amplification (LAMP) Assay during an Outbreak in Timor-Leste

Viruses ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1444
Author(s):  
Peter T. Mee ◽  
Shani Wong ◽  
Kim J. O’Riley ◽  
Felisiano da Conceição ◽  
Joanita Bendita da Costa Jong ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Lamp Assay ◽  
Fever Virus ◽  
Isothermal Amplification ◽  
Clinical Samples ◽  
Loop Mediated Isothermal Amplification ◽  
Field Verification ◽  
Extraction Step ◽  
Timor Leste

Recent outbreaks of African swine fever virus (ASFV) have seen the movement of this virus into multiple new regions with devastating impact. Many of these outbreaks are occurring in remote, or resource-limited areas, that do not have access to molecular laboratories. Loop-mediated isothermal amplification (LAMP) is a rapid point of care test that can overcome a range of inhibitors. We outline further development of a real-time ASFV LAMP, including field verification during an outbreak in Timor-Leste. To increase field applicability, the extraction step was removed and an internal amplification control (IAC) was implemented. Assay performance was assessed in six different sample matrices and verified for a range of clinical samples. A LAMP detection limit of 400 copies/rxn was determined based on synthetic positive control spikes. A colourmetric LAMP assay was also assessed on serum samples. Comparison of the LAMP assay to a quantitative polymerase chain reaction (qPCR) was performed on clinical ASFV samples, using both serum and oral/rectal swabs, with a substantial level of agreement observed. The further verification of the ASFV LAMP assay, removal of extraction step, implementation of an IAC and the assessment of a range of sample matrix, further support the use of this assay for rapid in-field detection of ASFV.

Detection of African swine fever virus by loop-mediated isothermal amplification

2010 ◽  
Vol 164 (1-2) ◽  
pp. 68-74 ◽  
Author(s):  
Heather E. James ◽  
K. Ebert ◽  
R. McGonigle ◽  
Scott M. Reid ◽  
Neil Boonham ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification

Rapid and visual detection of African swine fever virus antibody by using fluorescent immunochromatography test strip

Talanta ◽  
2020 ◽  
Vol 219 ◽  
pp. 121284 ◽  
Author(s):  
Chengfei Li ◽  
Xinglin He ◽  
Yong Yang ◽  
Wenxiao Gong ◽  
Kun Huang ◽  
...  
Keyword(s):  
Visual Detection ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Test Strip ◽  
Fever Virus ◽  
Virus Antibody

Genetic markers of the African swine fever virus

2020 ◽  
Vol 23 (04) ◽  
pp. 21-26
Author(s):  
A.K. Sibgatullova ◽  
◽  
M.E. Vlasov ◽  
I.A. Titov ◽  
◽  
...  
Keyword(s):  
Genetic Markers ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus
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