scholarly journals Substrate preferences, phylogenetic and biochemical properties of proteolytic bacteria present in the digestive tract of Nile tilapia

2021 ◽  
Author(s):  
Tanim Jabid Hossain ◽  
Mukta Das ◽  
Ferdausi Ali ◽  
Sumaiya Islam Chowdhury ◽  
Subrina Akter Zedny
Keyword(s):  
Digestive Tract ◽  
Nile Tilapia ◽  
Biochemical Properties ◽  
Substrate Preferences ◽  
Proteolytic Bacteria

scholarly journals Substrate preferences, phylogenetic and biochemical properties of proteolytic bacteria present in the digestive tract of Nile tilapia

2021 ◽  
Author(s):  
Tanim Jabid Hossain ◽  
Mukta Das ◽  
Ferdausi Ali ◽  
Sumaiya Islam Chowdhury ◽  
Subrina Akter Zedny
Keyword(s):  
Nile Tilapia ◽  
Molecular Taxonomy ◽  
Skim Milk ◽  
Biochemical Properties ◽  
Protease Production ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Substrate Preferences ◽  
Proteolytic Bacteria ◽  
Excellent Source

Abstract Vertebrate intestine appears an excellent source of proteolytic bacteria for industrial and probiotic use. We therefore aimed obtaining the gut-associated proteolytic species of Nile tilapia. We’ve isolated twenty six bacterial strains from its intestinal tract, seven of which showed exoprotease activity with the formation of clear halos on skim milk. Their depolymerization ability was further assessed on three distinct proteins including casein, gelatin and albumin. All the isolates could successfully hydrolyze the three substrates indicating relatively broad specificity of their secreted proteases. Molecular taxonomy and phylogeny of the proteolytic isolates were determined based on their 16S rRNA gene barcoding which suggested that the seven strains belong to three phyla viz. Firmicutes, Proteobacteria and Actinobacteria, distributed across the genera Priestia, Citrobacter, Pseudomonas, Stenotrophomonas, Burkholderia, Providencia and Micrococcus. The isolates were further characterized by a comprehensive study of their morphological, cultural, cellular and biochemical properties which were consistent with the phylogenetic annotations. To reveal their proteolytic capacity alongside substrate preferences, enzyme-production was determined by the diffusion assay. The Pseudomonas, Stenotrophomonas and Micrococcus isolates appeared most promising with maximum protease production on casein, gelatin and albumin media respectively. Our findings present valuable insights into the phylogenetic and biochemical properties of gut-associated proteolytic strains of Nile tilapia.

scholarly journals Substrate preferences, phylogenetic and biochemical properties of proteolytic bacteria present in the digestive tract of Nile tilapia (Oreochromis niloticus)

2021 ◽  
Vol 7 (4) ◽  
pp. 528-545
Author(s):  
Tanim Jabid Hossain ◽  
◽  
Mukta Das ◽  
Ferdausi Ali ◽  
Sumaiya Islam Chowdhury ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Molecular Taxonomy ◽  
Skim Milk ◽  
Biochemical Properties ◽  
Protease Production ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Substrate Preferences ◽  
Proteolytic Bacteria

<abstract> <p>Vertebrate intestine appears to be an excellent source of proteolytic bacteria for industrial and probiotic use. We therefore aimed at obtaining the gut-associated proteolytic species of Nile tilapia (<italic>Oreochromis niloticus</italic>). We have isolated twenty six bacterial strains from its intestinal tract, seven of which showed exoprotease activity with the formation of clear halos on skim milk. Their depolymerization ability was further assessed on three distinct proteins including casein, gelatin, and albumin. All the isolates could successfully hydrolyze the three substrates indicating relatively broad specificity of their secreted proteases. Molecular taxonomy and phylogeny of the proteolytic isolates were determined based on their 16S rRNA gene barcoding, which suggested that the seven strains belong to three phyla viz. Firmicutes, Proteobacteria, and Actinobacteria, distributed across the genera <italic>Priestia</italic>, <italic>Citrobacter</italic>, <italic>Pseudomonas</italic>, <italic>Stenotrophomonas</italic>, <italic>Burkholderia</italic>, <italic>Providencia</italic>, and <italic>Micrococcus</italic>. The isolates were further characterized by a comprehensive study of their morphological, cultural, cellular and biochemical properties which were consistent with the phylogenetic annotations. To reveal their proteolytic capacity alongside substrate preferences, enzyme-production was determined by the diffusion assay. The <italic>Pseudomonas</italic>, <italic>Stenotrophomonas</italic> and <italic>Micrococcus</italic> isolates appeared to be most promising with maximum protease production on casein, gelatin, and albumin media respectively. Our findings present valuable insights into the phylogenetic and biochemical properties of gut-associated proteolytic strains of Nile tilapia.</p> </abstract>

scholarly journals Substrate preferences, phylogenetic and biochemical properties of proteolytic bacteria present in the digestive tract of Nile tilapia

2021 ◽  
Author(s):  
Tanim Jabid Hossain ◽  
Mukta Das ◽  
Ferdausi Ali ◽  
Sumaiya Islam Chowdhury ◽  
Subrina Akter Zedny
Keyword(s):  
Nile Tilapia ◽  
Molecular Taxonomy ◽  
Skim Milk ◽  
Biochemical Properties ◽  
Protease Production ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Substrate Preferences ◽  
Proteolytic Bacteria ◽  
Excellent Source

Vertebrate intestine appears an excellent source of proteolytic bacteria for industrial and probiotic use. We therefore aimed obtaining the gut-associated proteolytic species of Nile tilapia. We have isolated twenty six bacterial strains from its intestinal tract, seven of which showed exoprotease activity with the formation of clear halos on skim milk. Their depolymerization ability was further assessed on three distinct proteins including casein, gelatin and albumin. All the isolates could successfully hydrolyze the three substrates indicating relatively broad specificity of their secreted proteases. Molecular taxonomy and phylogeny of the proteolytic isolates were determined based on their 16S rRNA gene barcoding which suggested that the seven strains belong to three phyla viz. Firmicutes, Proteobacteria and Actinobacteria, distributed across the genera Priestia, Citrobacter, Pseudomonas, Stenotrophomonas, Burkholderia, Providencia and Micrococcus. The isolates were further characterized by a comprehensive study of their morphological, cultural, cellular and biochemical properties which were consistent with the phylogenetic annotations. To reveal their proteolytic capacity alongside substrate preferences, enzyme-production was determined by the diffusion assay. The Pseudomonas, Stenotrophomonas and Micrococcus isolates appeared most promising with maximum protease production on casein, gelatin and albumin media respectively. Our findings present valuable insights into the phylogenetic and biochemical properties of gut-associated proteolytic strains of Nile tilapia.

A study of the histology of the digestive tract of the Nile tilapia

1999 ◽  
Vol 54 (3) ◽  
pp. 597-606 ◽  
Author(s):  
C. M. Morrison ◽  
J. R. Wright
Keyword(s):  
Digestive Tract ◽  
Nile Tilapia

scholarly journals Proteolytic bacteria in the lower digestive tract of poultry may affect avian influenza virus pathogenicity

2009 ◽  
Vol 88 (7) ◽  
pp. 1388-1393 ◽  
Author(s):  
M.D. King ◽  
M.N. Guentzel ◽  
B.P. Arulanandam ◽  
B. Lupiani ◽  
J.P. Chambers
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Digestive Tract ◽  
Proteolytic Bacteria ◽  
Lower Digestive Tract

Site‐specific profiles of biochemical properties in the larval digestive tract of Japanese rhinoceros beetle, Trypoxylus dichotomus (Coleoptera: Scarabaeidae)

2019 ◽  
Vol 23 (1) ◽  
pp. 33-43
Author(s):  
Noriko Wada ◽  
Noriyuki Iwabuchi ◽  
Michio Sunairi ◽  
Mutsuyasu Nakajima ◽  
Ryûtarô Iwata ◽  
...  
Keyword(s):  
Digestive Tract ◽  
Biochemical Properties ◽  
Site Specific ◽  
Rhinoceros Beetle ◽  
Trypoxylus Dichotomus

scholarly journals Biochemical Properties of Two Cinnamoyl Esterases Purified from a Lactobacillus johnsonii Strain Isolated from Stool Samples of Diabetes-Resistant Rats

2009 ◽  
Vol 75 (15) ◽  
pp. 5018-5024 ◽  
Author(s):  
Kin Kwan Lai ◽  
Graciela L. Lorca ◽  
Claudio F. Gonzalez
Keyword(s):  
Ferulic Acid ◽  
Genomic Analysis ◽  
Biochemical Properties ◽  
Insulin Production ◽  
Cinnamic Acids ◽  
Lactobacillus Johnsonii ◽  
Ethyl Ferulate ◽  
Substrate Preferences ◽  
Stool Samples ◽  
Low Dosage

ABSTRACT Cinnamic acids (i.e., ferulic and caffeic acids) that are esterified to the vegetable cell walls should be enzymatically released to be absorbed in a mammal's intestines. A low dosage of ferulic acid in rodent diets stimulates insulin production and alleviates symptoms caused by diabetes (M. Sri Balasubashini, R. Rukkumani, and V. P. Menon, Acta Diabetol. 40:118-122, 2003). Several lactic acid bacteria are able to display ferulic acid esterase (FAE) activity, suggesting that their probiotic activity could be, in part, mediated by the slow release of ferulic acid. In the present work, we describe the isolation of one strain identified as being Lactobacillus johnsonii that displayed strong FAE activity in stool samples from diabetes-resistant biobreeding rats. These animals are genetically susceptible to becoming diabetic but do not develop the disease. By using genomic analysis coupled to protein purification and catalytic screening, we were able to purify two proteins with FAE activity. The enzymes displayed 42% sequence identity and a broad range of substrate preferences. High affinities and catalytic efficiencies toward aromatic compounds such as ethyl ferulate (Km = 20 to 60 μM) and chlorogenic acid (Km = 10 to 50 μM) were observed. The strain isolated herein as well as the enzymes studied could be potentially useful for the formulation of probiotics to ameliorate diabetes symptoms.

scholarly journals Komposisi Bakteri Saluran Pencernaan Belut Rawa (Synbranchus Bengalensis)

bionature ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Arista Suci Andini ◽  
Faturrahman Faturrahman ◽  
Wahyu Hurriatul Khair
Keyword(s):  
Digestive Tract ◽  
Body Surface ◽  
Digestive Enzymes ◽  
The Body ◽  
Gram Positive ◽  
Bacterial Composition ◽  
Gram Positive Bacteria ◽  
Proteolytic Bacteria ◽  
Swamp Eel

Abstract. Eels are a group of snake- shaped fish belonging to the Synbranchidae. Bacteria in groups of fish such as eels can be found on the body surface and digestive tract. Some bacteria are pathogenic, while a number of other bacteria are beneficial, but researches in this topic has not been optimal. This study examined the bacterial composition of the digestive tract of swamp eel (Synbranchus bengalensis) using descriptive exploratory methods. The results showed that the composition of bacteria found in the digestive tract of swamp eels was diverse but dominated by gram-positive bacteria; BR2, BR3, BR4, BR5. In addition, some of these bacteria belong to the group of proteolytic bacteria; BR2 and BR4, lipolytic bacteria; BR2, and amylolytic bacteria; BR1 which hydrolyzes digestive enzymes can act as probiotics in the digestive tract of swamp eels (Synbranchus bengalensis).Keywords: swamp eels, microflora digestive, Synbranchus bengalensis

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