Subcutaneous Surgical Rat Models for the Evaluation of Tissue-Engineered Heart Valve Immunogenicity: A Systematic Review

2021 ◽  
Author(s):  
Steve Waqanivavalagi ◽  
Marcus Ground ◽  
Consuelo Alarcon ◽  
Paget Milsom ◽  
Jillian Cornish
Keyword(s):  
Systematic Review ◽  
Heart Valve ◽  
Rat Models ◽  
Tissue Engineered Heart Valve

scholarly journals Subcutaneous Surgical Rat Models for the Evaluation of Decellularised Heart Valve Immunogenicity: A Systematic Review

Materialia ◽  
2021 ◽  
pp. 101298
Author(s):  
Steve W F R Waqanivavalagi ◽  
Marcus B Ground ◽  
Consuelo Alarcon ◽  
Paget Milsom ◽  
Jillian Cornish
Keyword(s):  
Systematic Review ◽  
Heart Valve ◽  
Rat Models

scholarly journals Mechanical and Degradation Properties of Hybrid Scaffolds for Tissue Engineered Heart Valve (TEHV)

2021 ◽  
Vol 12 (1) ◽  
pp. 20
Author(s):  
Rabia Nazir ◽  
Arne Bruyneel ◽  
Carolyn Carr ◽  
Jan Czernuszka
Keyword(s):  
Heart Valve ◽  
Crosslinking Density ◽  
Biomechanical Properties ◽  
Collagen Type I ◽  
Type I ◽  
Aortic Heart Valve ◽  
Tissue Engineered Heart Valve ◽  
Human Aortic Valve ◽  
Degradation Properties ◽  
Hybrid Scaffolds

In addition to biocompatibility, an ideal scaffold for the regeneration of valvular tissue should also replicate the natural heart valve extracellular matrix (ECM) in terms of biomechanical properties and structural stability. In our previous paper, we demonstrated the development of collagen type I and hyaluronic acid (HA)-based scaffolds with interlaced microstructure. Such hybrid scaffolds were found to be compatible with cardiosphere-derived cells (CDCs) to potentially regenerate the diseased aortic heart valve. This paper focused on the quantification of the effect of crosslinking density on the mechanical properties under dry and wet conditions as well as degradation resistance. Elastic moduli increased with increasing crosslinking densities, in the dry and wet state, for parent networks, whereas those of interlaced scaffolds were higher than either network alone. Compressive and storage moduli ranged from 35 ± 5 to 95 ± 5 kPa and 16 ± 2 kPa to 113 ± 6 kPa, respectively, in the dry state. Storage moduli, in the dry state, matched and exceeded those of human aortic valve leaflets (HAVL). Similarly, degradation resistance increased with increasing the crosslinking densities for collagen-only and HA-only scaffolds. Interlaced scaffolds showed partial degradation in the presence of either collagenase or hyaluronidase as compared to when exposed to both enzymes together. These results agree with our previous findings that interlaced scaffolds were composed of independent collagen and HA networks without crosslinking between them. Thus, collagen/HA interlaced scaffolds have the potential to fill in the niche for designing an ideal tissue engineered heart valve (TEHV).

scholarly journals Tissue-Engineered Heart Valve with a Tubular Leaflet Design for Minimally Invasive Transcatheter Implantation

2015 ◽  
Vol 21 (6) ◽  
pp. 530-540 ◽  
Author(s):  
Ricardo Moreira ◽  
Thaddaeus Velz ◽  
Nuno Alves ◽  
Valentine N. Gesche ◽  
Axel Malischewski ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Heart Valve ◽  
Transcatheter Implantation ◽  
Tissue Engineered Heart Valve

scholarly journals Decellularized Tissue-Engineered Heart Valve Leaflets with Recellularization Potential

2013 ◽  
Vol 19 (5-6) ◽  
pp. 759-769 ◽  
Author(s):  
Zeeshan H. Syedain ◽  
Allison R. Bradee ◽  
Stefan Kren ◽  
Doris A. Taylor ◽  
Robert T. Tranquillo
Keyword(s):  
Heart Valve ◽  
Tissue Engineered Heart Valve ◽  
Decellularized Tissue

Large Displacement Flexural Properties of Tissue Engineered Heart Valve Scaffolds

2000 ◽  
Author(s):  
Michael S. Sacks ◽  
Sanjay Kaushal ◽  
John E. Mayer
Keyword(s):  
Heart Valve ◽  
Heart Valves ◽  
Critical Test ◽  
Bioprosthetic Heart Valves ◽  
Property Evaluation ◽  
Fundamental Information ◽  
Tissue Engineered Heart Valve ◽  
Valve Prostheses ◽  
Tissue Engineered Heart Valves

Abstract The need for improved heart valve prostheses is especially critical in pediatric applications, where growth and remodeling are essential. Tissue engineered heart valves (TEHV) have functioned in the pulmonary circulation of growing lambs for up to four months [1], and thus can potentially overcome limitations of current bioprosthetic heart valves. Despite these promising results, significant questions remain. In particular, the role of scaffold mechanical properties in optimal extra-cellular matrix development, as well as TEHV durability, are largely unexplored. We have previously demonstrated flexure testing as a sensitive and critical test for BHV tissue mechanical property evaluation [2]. The following study was conducted to determine the feasibility of using this technique to provide fundamental information required for optimizing TEHV scaffold designs.

P4739Novel tissue-engineered heart valve without any foreign materials

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
Y Takewa ◽  
Y Nakayama ◽  
J Shimamura ◽  
N Katagiri ◽  
E Tatsumi
Keyword(s):  
Heart Valve ◽  
Animal Experiments ◽  
Large Animal ◽  
Connective Tissues ◽  
Autologous Tissue ◽  
Aortic Bypass ◽  
Tissue Engineered Heart Valve ◽  
Foreign Materials ◽  
Bioprosthetic Valves

Abstract Background We are developing a novel autologous tissue-engineered heart valve with a unique in-body tissue engineering. This is expected to be a viable bioprosthesis keeping better biocompatibility. Purpose We developed a conduit-type valve without any foreign materials and tested the feasibility and long-term availability in large animal experiments. Methods We created plastic molds for Biovalves with 3D printer easily and quickly considering the recipient character. We embedded them in the subcutaneous spaces of adult goats about 2 months. After extracting the molds with the tissue en-block and removing the plastic molds only, Biovalve with tri-leaflets similar to those of the native valves were constituted from completely autologous connective tissues and fibroblasts. Total 21 conduit-type Biovalves were implanted in the apico-aortic bypass or the pulmonary artery of goats, (8 and 13, respectively). No anticoagurants were used after implantation. Results The valves were successfully implanted and showed smooth movement of the leaflets with a little regurgitation in angiogram, and the maximum duration reached to 3 years 7 months. Histological examination of the Biovalves showed the autologous cells covering the laminar surface of the valve leaflets as the endothelium and also getting inside to construct characteristic tissues like native leaflets. Conclusion The valves have a potential to be used for viable bioprosthetic valves and to keep better function and biocompatibility longer than current ones.

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