scholarly journals Liquid-Crystalline Lipid Phase Transitions in Lipid Droplets Selectively Remodel the Ld Proteome

2021 ◽  
Author(s):  
Sean Rogers ◽  
Long Gui ◽  
Anastasiia Kovalenko ◽  
Evan Reetz ◽  
Daniela Nicastro ◽  
...  
Keyword(s):  
Phase Transitions ◽  
Lipid Droplets ◽  
Liquid Crystalline ◽  
Lipid Phase ◽  
Lipid Phase Transitions

scholarly journals Liquid-crystalline lipid phase transitions in lipid droplets selectively remodel the LD proteome

2021 ◽  
Author(s):  
Sean Rogers ◽  
Long Gui ◽  
Anastasiia Kovalenko ◽  
Evan Reetz ◽  
Daniela Nicastro ◽  
...  
Keyword(s):  
Phase Transitions ◽  
Lipid Droplets ◽  
Liquid Crystalline ◽  
Electron Tomography ◽  
Organelle Transport ◽  
Lipid Phase ◽  
Sterol Esters ◽  
Lipid Phase Transitions ◽  
Liquid Crystalline Phases ◽  
Glucose Restriction

Lipid droplets (LDs) are reservoirs for triglycerides (TGs) and sterol-esters (SEs). How lipids are organized within LDs and influence the LD proteome remains unclear. Using in situ cryo-electron tomography, we show that glucose restriction triggers lipid phase transitions within LDs generating liquid-crystalline lattices inside them. Mechanistically, this requires TG lipolysis, which alters LD neutral lipid composition and promotes SE transition to a liquid-crystalline phase. Fluorescence imaging and proteomics further reveal that LD liquid-crystalline lattices selectively remodel the LD proteome. Some canonical LD proteins including Erg6 re-localize to the ER network, whereas others remain on LDs. Model peptide LiveDrop also redistributes from LDs to the ER, suggesting liquid-crystalline-phases influence ER-LD inter-organelle transport. Proteomics also indicates glucose restriction elevates peroxisome lipid oxidation, suggesting TG mobilization provides fatty acids for cellular energetics. This suggests glucose restriction drives TG mobilization, which alters the phase properties of LD lipids and selectively remodels the LD proteome.

Lipid phase transitions measured in intact cells with fourier transform infrared spectroscopy

Cryobiology ◽  
1989 ◽  
Vol 26 (1) ◽  
pp. 76-84 ◽  
Author(s):  
John H. Crowe ◽  
Folkert A. Hoekstra ◽  
Lois M. Crowe ◽  
Thomas J. Anchordoguy ◽  
Erma Drobnis
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Phase Transitions ◽  
Fourier Transform Infrared Spectroscopy ◽  
Fourier Transform Infrared ◽  
Lipid Phase ◽  
Intact Cells ◽  
Lipid Phase Transitions

Lipid phase transitions in cat oocytes supplemented with deuterated fatty acids

2021 ◽  
Author(s):  
Konstantin A. Okotrub ◽  
Svetlana V. Okotrub ◽  
Valentina I. Mokrousova ◽  
Sergei Y. Amstislavsky ◽  
Nikolay V. Surovtsev
Keyword(s):  
Fatty Acids ◽  
Phase Transitions ◽  
Lipid Phase ◽  
Lipid Phase Transitions

Lipid phase transitions and drug interactions

1974 ◽  
Vol 363 (1) ◽  
pp. 54-69 ◽  
Author(s):  
Bryan R. Cater ◽  
Dennis Chapman ◽  
Susan M. Hawes ◽  
John Saville
Keyword(s):  
Phase Transitions ◽  
Drug Interactions ◽  
Lipid Phase ◽  
Lipid Phase Transitions
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