Modified Simultaneous Saccharification and Co-Fermentation of DLC Pretreated Corn Stover for High-Titer Cellulosic Ethanol Production Without Water Washing or Detoxifying Pretreated Biomass

2021 ◽  
Author(s):  
Xinchuan Yuan ◽  
Guannan Shen ◽  
Sitong Chen ◽  
Xiangxue Chen ◽  
Mingjie Jin
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Cellulosic Ethanol ◽  
High Titer ◽  
Pretreated Corn Stover ◽  
Water Washing ◽  
Pretreated Biomass ◽  
Simultaneous Saccharification

Ethanol Production from Corn Stover Using Soaking Pretreatment

2010 ◽  
Vol 171-172 ◽  
pp. 261-265
Author(s):  
Zhuang Zuo ◽  
Xiu Shan Yang
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Pichia Stipitis ◽  
Liquid Ratio ◽  
Mild Conditions ◽  
Whole Process ◽  
Conversion Rates ◽  
Mild Temperature ◽  
Simultaneous Saccharification

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH，50°C，48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% delignification, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous saccharification and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.

Expression of serpins by Clostridium thermocellum and simultaneous saccharification of lignocellulosic biomass to enhance ethanol production

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 8662-8676
Author(s):  
Maria Mushtaq ◽  
Muhammad Javaid Asad ◽  
Muhammad Zeeshan Hyder ◽  
Syed Muhammad Saqlan Naqvi ◽  
Saad Imran Malik ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Wheat Straw ◽  
Corn Stover ◽  
Rice Straw ◽  
Clostridium Thermocellum ◽  
Ethanol Yield ◽  
Biofuel Production ◽  
Yeast Fermentation ◽  
Second Generation Bioethanol ◽  
Simultaneous Saccharification

Utilization of biomass for production of second generation bioethanol was considered as a way to reduce burdens of fossil fuel in Pakistan. The materials wheat straw, rice straw, cotton stalk, corn stover, and peel wastes were used in this experiment. Various parameters, such as acidic and alkali pretreatment, enzymatic hydrolysis by cellulases, and effect of proteases inhibitors on ethanol production, were examined. Fermentation was completed by the yeasts Saccharomyces cerevisiae and Clostridium thermocellum separately, and their ethanol production were compared and maximum ethanol yield was obtained with wheat straw i.e.,11.3 g/L by S. cerevisiae and 8.5 g/L by C. thermocellum. Results indicated that a higher quantity of sugar was obtained from wheat straw (19.6 ± 1.6 g/L) followed by rice straw (17.6 ± 0.6 g/L) and corn stover (16.1 ± 0.9 g/L) compared to the other evaluated biomass samples. A higher yield of ethanol (11.3 g/L) was observed when a glucose concentration of 21.7 g/L was used, for which yeast fermentation efficiency was 92%. Results also revealed the increased in ethanol production (93%) by using celluases in combination with recombinant Serine protease inhibitors from C. thermocellum. It is expected that the use of recombinant serpins with cellulases will play a major role in the biofuel production by using agricultural biomass. This will also help in the economics of the biofuel.

Simultaneous saccharification and co-fermentation of glucose and xylose in steam-pretreated corn stover at high fiber content with Saccharomyces cerevisiae TMB3400

2006 ◽  
Vol 126 (4) ◽  
pp. 488-498 ◽  
Author(s):  
Karin Öhgren ◽  
Oskar Bengtsson ◽  
Marie F. Gorwa-Grauslund ◽  
Mats Galbe ◽  
Bärbel Hahn-Hägerdal ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Corn Stover ◽  
Fiber Content ◽  
High Fiber ◽  
Pretreated Corn Stover ◽  
Simultaneous Saccharification

A lignin-first strategy to recover hydroxycinnamic acids and improve cellulosic ethanol production from corn stover

2020 ◽  
Vol 138 ◽  
pp. 105579 ◽  
Author(s):  
Patrick A. Johnston ◽  
Haoqin Zhou ◽  
Alvina Aui ◽  
Mark Mba Wright ◽  
Zhiyou Wen ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Cellulosic Ethanol ◽  
Hydroxycinnamic Acids
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