Molecularly Imprinted Polymer as Sorbent Phase for Disposable Pipette Extraction: A Potential Approach for Creatinine Analysis in Human Urine Samples

2021 ◽  
Author(s):  
Izadora Carvalho Gomes ◽  
Rafael Oliveira Martins ◽  
Lucas Santos Machado ◽  
Alessandra Timotéo Cardoso ◽  
Paulo Sérgio de Souza ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Human Urine ◽  
Urine Samples ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Potential Approach ◽  
Sorbent Phase ◽  
Human Urine Samples ◽  
Disposable Pipette Extraction

Microextraction by packed sorbent using a new restricted molecularly imprinted polymer for the determination of estrogens from human urine samples

2019 ◽  
Vol 150 ◽  
pp. 104162 ◽  
Author(s):  
Hanna Leijoto de Oliveira ◽  
Leila Suleimara Teixeira ◽  
Laíse Aparecida Fonseca Dinali ◽  
Bruna Carneiro Pires ◽  
Nathália Soares Simões ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Human Urine ◽  
Urine Samples ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Human Urine Samples ◽  
Microextraction By Packed Sorbent ◽  
Packed Sorbent

Development of pipette tip-based on molecularly imprinted polymer micro-solid phase extraction for selective enantioselective determination of (−)-(2S,4R) and (+)-(2R,4S) ketoconazole in human urine samples prior to HPLC-DAD

2016 ◽  
Vol 8 (20) ◽  
pp. 4075-4085 ◽  
Author(s):  
Ricky Cássio Santos da Silva ◽  
Valdir Mano ◽  
Arnaldo César Pereira ◽  
Eduardo Costa de Figueiredo ◽  
Keyller Bastos Borges
Keyword(s):  
Human Urine ◽  
Solid Phase ◽  
Urine Samples ◽  
Preparation Technique ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Human Urine Samples ◽  
Sample Preparation Technique ◽  
Pipette Tip

A simple and selective sample preparation technique employing PT-MIP-μ-SPE coupled to HPLC/DAD was developed for the determination of the cis-enantiomers of ketoconazole in human urine samples.

scholarly journals A Molecularly Imprinted Polymer for Selective Extraction of Phenolic Acids from Human Urine

2021 ◽  
Vol 11 (4) ◽  
pp. 1577
Author(s):  
Marco Mora-Granados ◽  
David González-Gómez ◽  
Jin Su Jeong ◽  
Alejandrina Gallego-Picó
Keyword(s):  
Phenolic Acids ◽  
Molecularly Imprinted Polymer ◽  
Human Urine ◽  
Limit Of Detection ◽  
Ph Dependence ◽  
Selective Extraction ◽  
Screening Methods ◽  
Specific Method ◽  
Imprinted Polymer ◽  
Molecularly Imprinted

Studies for monitoring the bioavailability of dietary flavonoid compounds generate great interest. Among them, low-molecular-weight phenolic acids, secondary metabolites present in colonic catabolism and urinary excretion, have been proposed as biomarkers of polyphenol intake. Using 4-hydroxyphenylacetic acid as a template, a molecularly imprinted polymer (MIP) was synthesized for selective extraction of these hydroxylated metabolites from human urine samples and posterior analysis in an HPLC-DAD-MS system. Polymers were characterized by Scanning electron microscopy (SEM), Attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), Brunauer-Emmett-Teller (BET) method, and binding experiments. MIP presents specific recognition ability for template and analogues molecules. This capacity of recognition and the pH dependence of the binding strength was also studied. The method was validated over a concentration range of 0.25–40 mg/L, r2 > 0.995. In the optimized conditions, the recovery value was 94% with RSD 1.2%. The Limit of Detection (LOD) and Limit of Quantification (LOQ) were 1.22 and 3.69 mg/L, respectively. In our knowledge, it is the first time that this methodology is applied to analyze urinary catabolites of the polyphenol compound and to provide a specific method and simple analysis alternative. The selective extraction of these metabolites improves the application and results obtained by other less sensitive analysis methods than the validation method. It also facilitates the development of new screening methods.

Synthesis of a molecularly imprinted polymer for the isolation of 1-hydroxypyrene in human urine

Talanta ◽  
2015 ◽  
Vol 143 ◽  
pp. 71-76 ◽  
Author(s):  
M. Serrano ◽  
M. Bartolomé ◽  
A. Gallego-Picó ◽  
R.M. Garcinuño ◽  
J.C. Bravo ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Human Urine ◽  
Imprinted Polymer ◽  
Molecularly Imprinted
Sign in / Sign up

Export Citation Format

Share Document