Precise Design Strategy Of  Smart Extracellular Matrix Based on CRISPR/Cas9 for Regulating Neural Stem Cell Function

2021 ◽  
Author(s):  
Yuanxin Zhai ◽  
Lingyan Yang ◽  
Wenlong Zheng ◽  
Quanwei Wang ◽  
Zhanchi Zhu ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Function ◽  
Design Strategy

scholarly journals Roles for HIF-1α in neural stem cell function and the regenerative response to stroke

2012 ◽  
Vol 227 (2) ◽  
pp. 410-417 ◽  
Author(s):  
Lee Anna Cunningham ◽  
Kate Candelario ◽  
Lu Li
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Function ◽  
Regenerative Response

scholarly journals Extracellular matrix and the neural stem cell niche

2011 ◽  
Vol 71 (11) ◽  
pp. 1006-1017 ◽  
Author(s):  
Ilias Kazanis ◽  
Charles ffrench-Constant
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Stem Cell Niche ◽  
Neural Stem Cell Niche ◽  
Cell Niche

scholarly journals Single-Cell Transcriptomics and Fate Mapping of Ependymal Cells Reveals an Absence of Neural Stem Cell Function

Cell ◽  
2018 ◽  
Vol 173 (4) ◽  
pp. 1045-1057.e9 ◽  
Author(s):  
Prajay T. Shah ◽  
Jo A. Stratton ◽  
Morgan Gail Stykel ◽  
Sepideh Abbasi ◽  
Sandeep Sharma ◽  
...  
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Neural Stem Cell ◽  
Cell Function ◽  
Ependymal Cells ◽  
Fate Mapping

Overcoming the Aging Systemic Milieu to Restore Neural Stem Cell Function

2011 ◽  
Vol 14 (6) ◽  
pp. 681-684 ◽  
Author(s):  
Andrew R. Mendelsohn ◽  
James W. Larrick
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Function

scholarly journals Fractone bulbs derive from ependymal cells and their laminin composition influence the stem cell niche in the subventricular zone

2016 ◽  
Author(s):  
Marcos Assis Nascimento ◽  
Lydia Sorokin ◽  
Tatiana Coelho-Sampaio
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Neural Stem Cells ◽  
Neural Stem Cell ◽  
Adult Neurogenesis ◽  
Subventricular Zone ◽  
Stem Cell Niche ◽  
Ependymal Cells ◽  
Cell Niche

AbstractFractones are extracellular matrix structures in the neural stem cell niche of the subventricular zone (SVZ), where they appear as round deposits named bulbs or thin branching lines called stems. Their cellular origin and what determines their localization at this site is poorly studied and it remains unclear whether they influence neural stem and progenitor cells formation, proliferation and/or maintenance. To address these questions, we analyzed whole mount preparations of the lateral ventricle by confocal microscopy using different extracellular matrix and cell markers. We found that bulbs are rarely connected to stems and that they contain laminin α5 and α2 chains, respectively. Fractone bulbs were profusely distributed throughout the SVZ and appeared associated with the center of pinwheels, a critical site for adult neurogenesis. We demonstrate that bulbs appear at the apical membrane of ependymal cells at the end of the first week after birth. The use of transgenic mice lacking laminin α5 gene expression (Lama5) in endothelium and in FoxJ1-expressing ependymal cells, revealed ependymal cells as the source of laminin α5-containing fractone bulbs. Loss of laminin α5 from bulbs correlated with a 60% increase in cell proliferation, as determined by PH3 staining, and with a selective reduction in the number of quiescent neural stem cells in the SVZ. These results indicate that fractones are a key component of the SVZ and suggest that laminin α5 modulates the physiology of the neural stem cell niche.Significance StatementOur work unveils key aspects of fractones, extracellular matrix structures present in the SVZ that still lack a comprehensive characterization. We show that fractones extensively interact with neural stem cells, whereas some of them are located precisely at pinwheel centers, which are hotspots for adult neurogenesis. Our results also demonstrate that fractones increase in size during aging and that their interactions with NSPCs become more complex in old mice. Lastly, we show that fractone bulbs are produced by ependymal cells and that their laminin content regulates neural stem cells.

scholarly journals Stepwise Adipogenesis of Decellularized Cellular Extracellular Matrix Regulates Adipose Tissue-Derived Stem Cell Migration and Differentiation

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Ziang Zhang ◽  
Rongmei Qu ◽  
Tingyu Fan ◽  
Jun Ouyang ◽  
Feng Lu ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Cell Function ◽  
Adipogenic Differentiation ◽  
Integrin Subunit ◽  
Migration Ability ◽  
Cell Behaviors ◽  
Adipose Tissue Engineering ◽  
Regulatory Effects

Microenvironmental factors can modulate the cellular status of adipose tissue-derived stem cells (ASCs). In response to microenvironmental changes, cells can remodel extracellular matrix (ECM) proteins, which play an important role in regulating cell behaviors. During adipogenic differentiation, ECM components secreted from ASCs remodel dramatically. To evaluate the role of stepwise adipogenesis-induced cellular secretion of ECM on the behavior of ASCs, we cultured ASCs in growth and adipogenic media, and ECM secreted from cells was characterized and decellularized. The ASCs were then reseeded on decellularized ECM (d-ECM) to determine the regulatory effects of ECM on cellular behaviors. During adipogenesis, cell-secreted ECM underwent remodeling characterized by conversion from fibronectin-rich ECM to laminin-rich ECM. The cellular status of ASCs was tested after reseeding on decellularized ECM. When reseeded on growth d-ECM, ASCs exhibited greater migration ability. In contrast, ASCs seeded on adipogenic d-ECM underwent adipogenic differentiation. In addition, integrin subunit αv and integrins α6 and α7 were detected at significantly greater levels in ASCs cultured on growth and adipogenic d-ECM, respectively, suggesting that integrins play an important role in ASC migration and adipogenesis. This study demonstrated that stepwise adipogenesis-induced ECM production plays an important role in ASC migration and differentiation. In addition, this study provided a strategy to achieve precise regulation of stem cell function in adipose tissue engineering.

Abstract PR15: Acting at a distance: Medulloblastoma-secreted ligands disrupt normal neural stem cell function

2020 ◽  
Author(s):  
Alexander Gont ◽  
Jaclin V. Simonetta ◽  
Jenna Park ◽  
Alice R. Shan ◽  
Michael J. Borrett ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Function
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