Exploring Synergistic Effect of Herbal Pair of Stephania Tetrandra and Astragalus Membranaceus on Treatment of Nephrotic Syndrome by Using Integrated Metabolomics and Pharmacodynamics

2021 ◽  
Author(s):  
Xiaoli Wang ◽  
Mengxue Yao ◽  
Shanshan Zhang ◽  
Bin Wang ◽  
Yanquan Han ◽  
...  
Keyword(s):  
Nephrotic Syndrome ◽  
Synergistic Effect ◽  
Astragalus Membranaceus ◽  
Integrated Metabolomics ◽  
Stephania Tetrandra

scholarly journals Integrated metabolomics and transcriptomics study of traditional herb Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao reveals global metabolic profile and novel phytochemical ingredients

BMC Genomics ◽  
2020 ◽  
Vol 21 (S10) ◽  
Author(s):  
Xueting Wu ◽  
Xuetong Li ◽  
Wei Wang ◽  
Yuanhong Shan ◽  
Cuiting Wang ◽  
...  
Keyword(s):  
Neutral Loss ◽  
Herbal Remedies ◽  
Astragalus Membranaceus ◽  
Bioinformatics Pipeline ◽  
Phenylpropanoid Biosynthesis ◽  
Rich Natural Resource ◽  
Order Of Magnitude ◽  
Transcriptomics Data ◽  
The Rich ◽  
Integrated Metabolomics

Abstract Background Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao is one of the most common herbs widely used in South and East Asia, to enhance people’s health and reinforce vital energy. Despite its prevalence, however, the knowledge about phytochemical compositions and metabolite biosynthesis in Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao is very limited. Results An integrated metabolomics and transcriptomics analysis using state-of-the-art UPLC-Q-Orbitrap mass spectrometer and advanced bioinformatics pipeline were conducted to study global metabolic profiles and phytochemical ingredients/biosynthesis in Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao. A total of 5435 metabolites were detected, from which 2190 were annotated, representing an order of magnitude increase over previously known. Metabolic profiling of Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao tissues found contents and synthetic enzymes for phytochemicals were significantly higher in leaf and stem in general, whereas the contents of the main bioactive ingredients were significantly enriched in root, underlying the value of root in herbal remedies. Using integrated metabolomics and transcriptomics data, we illustrated the complete pathways of phenylpropanoid biosynthesis, flavonoid biosynthesis, and isoflavonoid biosynthesis, in which some were first reported in the herb. More importantly, we discovered novel flavonoid derivatives using informatics method for neutral loss scan, in addition to inferring their likely synthesis pathways in Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao. Conclusions The current study represents the most comprehensive metabolomics and transcriptomics analysis on traditional herb Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao. We demonstrated our integrated metabolomics and transcriptomics approach offers great potentials in discovering novel metabolite structure and associated synthesis pathways. This study provides novel insights into the phytochemical ingredients, metabolite biosynthesis, and complex metabolic network in herbs, highlighting the rich natural resource and nutritional value of traditional herbal plants.

scholarly journals Integrated Metabolomics and Network Pharmacology to Establish the Action Mechanism of Qingrekasen Granule for Treating Nephrotic Syndrome

2021 ◽  
Vol 12 ◽  
Author(s):  
Yanfen Duan ◽  
Dongning Zhang ◽  
Yan Ye ◽  
Sili Zheng ◽  
Ping Huang ◽  
...  
Keyword(s):  
Nephrotic Syndrome ◽  
Molecular Docking ◽  
Metabolic Pathways ◽  
Underlying Mechanism ◽  
Network Pharmacology ◽  
Glutamate Metabolism ◽  
Active Components ◽  
Glomerular Permeability ◽  
Expression Levels ◽  
Integrated Metabolomics

Nephrotic syndrome (NS) is a clinical syndrome resulting from abnormal glomerular permeability, mainly manifesting as edema and proteinuria. Qingrekasen granule (QRKSG), a Chinese Uyghur folk medicine, is a single-flavor preparation made from chicory (Cichorium intybus L.), widely used in treating dysuria and edema. Chicory, the main component in QRKSG, effectively treats edema and protects kidneys. However, the active components in QRKSG and its underlying mechanism for treating NS remain unclear. This study explored the specific mechanism and composition of QRKSG on an NS rat model using integrated metabolomics and network pharmacology. First, metabolomics explored the relevant metabolic pathways impacted by QRKSG in the treatment of NS. Secondly, network pharmacology further explored the possible metabolite targets. Afterward, a comprehensive network was constructed using the results from the network pharmacology and metabolomics analysis. Finally, the interactions between the active components and targets were predicted by molecular docking, and the differential expression levels of the target protein were verified by Western blotting. The metabolomics results showed “D-Glutamine and D-glutamate metabolism” and “Alanine, aspartate, and glutamate metabolism” as the main targeted metabolic pathways for treating NS in rats. AKT1, BCL2L1, CASP3, and MTOR were the core QRKSG targets in the treatment of NS. Molecular docking revealed that these core targets have a strong affinity for flavonoids, terpenoids, and phenolic acids. Moreover, the expression levels of p-PI3K, p-AKT1, p-mTOR, and CASP3 in the QRKSG group significantly decreased, while BCL2L1 increased compared to the model group. These findings established the underlying mechanism of QRKSG, such as promoting autophagy and anti-apoptosis through the expression of AKT1, CASP3, BCL2L1, and mTOR to protect podocytes and maintain renal tubular function.

Cytokine polymorphisms and nephrotic syndrome

2002 ◽  
Vol 102 (5) ◽  
pp. 513 ◽  
Author(s):  
PETER W. MATHIESON
Keyword(s):  
Nephrotic Syndrome
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