In-vivo Sonoporation Effect Under the Presence of a Large Amount of Micro-Nano Bubbles in Swine Liver

2021 ◽  
Author(s):  
Yutaka Ueno ◽  
Shuji Kariya ◽  
Miyuki Nakatani ◽  
Yasuyuki Ono ◽  
Takuji Maruyama ◽  
...  
Keyword(s):  
Swine Liver

scholarly journals Design and realization of a normothermic perfusion system for laboratory tests on pig liver

2020 ◽  
Vol 43 (1) ◽  
pp. 3-9
Author(s):  
Lucrezia Mazzantini ◽  
Mattia Dimitri ◽  
Fabio Staderini ◽  
Fabio Cianchi ◽  
Andrea Corvi
Keyword(s):  
Ex Vivo ◽  
Infrared Camera ◽  
Open Circuit ◽  
Physiological Solution ◽  
Fluid Temperature ◽  
Pig Liver ◽  
Swine Liver ◽  
Physiological Values ◽  
University Of Florence

Ex vivo testing is a fundamental step in the development of new medical devices; indeed without it, it is impossible to proceed with in vivo tests. At the University of Florence, a robotic tool for microwave thermal ablation is under development. Up to now, the thermoablation tests for the validation of the tool were carried out on non-perfused ex vivo livers, providing results that inevitably differ from those obtainable with an in vivo liver. The aim is to design, and consequently create, a compact and transportable system which allows to perfuse a swine liver with physiological solution and heparin. This device should also allow the organ to be transported from the explantation place to the laboratory, keeping it under normothermal condition. The perfusor was designed to simulate the physiological flow within the liver in the most realistic way possible. The design, construction, and optimization of the perfusor have been addressed using the physiological values of hepatic flow and pressure identified in the literature, neglecting in the first instance any load losses. Therefore, open circuit tests were conducted, validated through perfusion tests on freshly explanted pig liver; during these tests, the surface temperature of the organ was recorded using an infrared camera, and the fluid temperature was verified using an immersion probe. The perfusion test showed a good alignment with the open circuit tests, demonstrating the validity of the simplifications adopted to treat the complex vascular structure of the liver.

scholarly journals Comparison of CT Fluoroscopy-Guided Manual and CT-Guided Robotic Positioning System for In Vivo Needle Placements in Swine Liver

2014 ◽  
Vol 38 (5) ◽  
pp. 1252-1260 ◽  
Author(s):  
F. Cornelis ◽  
H. Takaki ◽  
M. Laskhmanan ◽  
J. C. Durack ◽  
J. P. Erinjeri ◽  
...  
Keyword(s):  
Positioning System ◽  
Ct Fluoroscopy ◽  
Ct Guided ◽  
Swine Liver

scholarly journals Vandetanib-eluting Radiopaque Beads: In vivo Pharmacokinetics, Safety and Toxicity Evaluation following Swine Liver Embolization

Theranostics ◽  
2017 ◽  
Vol 7 (8) ◽  
pp. 2164-2176 ◽  
Author(s):  
Alban Denys ◽  
Peter Czuczman ◽  
David Grey ◽  
Zainab Bascal ◽  
Rhys Whomsley ◽  
...  
Keyword(s):  
Toxicity Evaluation ◽  
Swine Liver ◽  
Safety And Toxicity ◽  
Liver Embolization ◽  
In Vivo Pharmacokinetics

Microwave Ablation with Multiple Simultaneously Powered Small-gauge Triaxial Antennas: Results from an in Vivo Swine Liver Model

Radiology ◽  
2007 ◽  
Vol 244 (1) ◽  
pp. 151-156 ◽  
Author(s):  
Christopher L. Brace ◽  
Paul F. Laeseke ◽  
Lisa A. Sampson ◽  
Tina M. Frey ◽  
Daniel W. van der Weide ◽  
...  
Keyword(s):  
Microwave Ablation ◽  
Liver Model ◽  
Swine Liver ◽  
Small Gauge

scholarly journals Toxicokinetics of citreoviridinin vivoandin vitro

2019 ◽  
Author(s):  
Yosuke Uchiyama ◽  
Masahiko Takino ◽  
Michiko Noguchi ◽  
Nozomi Shiratori ◽  
Naoki Kobayashi ◽  
...  
Keyword(s):  
Human Cell Line ◽  
Volume Of Distribution ◽  
The Body ◽  
Long Duration ◽  
Permeability Study ◽  
Elimination Half ◽  
Metabolite Profiles ◽  
Swine Liver

AbstractCitreoviridin (CIT) produced byPenicillium citreonigrumas a secondary metabolite is a yellow rice toxin that has been reported to be related to acute cardiac beriberi; however, its toxicokinetics remain unclear. The present study elucidated the toxicokinetics through swinein vivoexperiments and predicted the human toxicokinetics by a comparison with findings fromin vitroexperiments. Swinein vivoexperiments revealed that CIT had a high bioavailability of more than 90%. In addition, it showed a large volume of distribution (1.005 ± 0.195 L/kg) and long elimination half-life (17.7 ± 3.3 h) in intravenous. These results suggested the possibility of a slow metabolism of CIT. An intestinal permeability study using the human cell line Caco-2 showed that CIT had a high permeability coefficient, suggesting it would be easily absorbed in human intestine, similar to its absorption in swine. The metabolite profiles were investigated by incubating CIT with S9 obtained from swine and humans. Hydroxylation, methylation, desaturation and dihydroxylation derivatives were detected as the predominant metabolites, and CIT glucuronide was produced slowly compared with above metabolites. A comparison of the peak area ratios obtained using quadrupole time-of-flight mass spectrometer showed that the rates of all of the main metabolites except for glucuronide produced using human S9 were three-fold higher than those obtained using swine S9. Furthermore, the elimination of CIT using human S9 was more rapid than when using swine S9, indicating that CIT would be metabolized faster in humans than in swine. Thesein vivoresults suggested that CIT is easily absorbed in swine and persists in the body for a long duration. Furthermore, the CIT metabolism appeared to be faster in human liver than in swine liverin vitro, although the bioavailability of CIT was predicted to be similarly high in humans as in swine.

Fine Structural Changes in the Microvasculature of the Mouse Pinna: Aging and Radiation Injury

1974 ◽  
Vol 32 ◽  
pp. 54-55
Author(s):  
S. Phyllis Steamer ◽  
Rosemarie L. Devine
Keyword(s):  
Structural Changes ◽  
Radiation Treatment ◽  
Arterial Stenosis ◽  
Ultrastructural Changes ◽  
Vascular Effects ◽  
Microvascular Changes ◽  
Surrounding Connective Tissue ◽  
Fission Neutrons ◽  
Total Body

The importance of radiation damage to the skin and its vasculature was recognized by the early radiologists. In more recent studies, vascular effects were shown to involve the endothelium as well as the surrounding connective tissue. Microvascular changes in the mouse pinna were studied in vivo and recorded photographically over a period of 12-18 months. Radiation treatment at 110 days of age was total body exposure to either 240 rad fission neutrons or 855 rad 60Co gamma rays. After in vivo observations in control and irradiated mice, animals were sacrificed for examination of changes in vascular fine structure. Vessels were selected from regions of specific interest that had been identified on photomicrographs. Prominent ultrastructural changes can be attributed to aging as well as to radiation treatment. Of principal concern were determinations of ultrastructural changes associated with venous dilatations, segmental arterial stenosis and tortuosities of both veins and arteries, effects that had been identified on the basis of light microscopic observations. Tortuosities and irregularly dilated vein segments were related to both aging and radiation changes but arterial stenosis was observed only in irradiated animals.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Ultrastructural Correlations between Clonal Human Tumor Colonies and in Vivo Neoplasms

1982 ◽  
Vol 40 ◽  
pp. 210-211
Author(s):  
M.J. Murphy ◽  
R.R. Price ◽  
J.C. Sloman
Keyword(s):  
Cultured Cells ◽  
Human Tumor ◽  
Cell Type ◽  
Tumor Mass ◽  
Initial Cell ◽  
Cloning Assay ◽  
Human Tumor Cloning ◽  
The Relationship

The in vitro human tumor cloning assay originally described by Salmon and Hamburger has been applied recently to the investigation of differential anti-tumor drug sensitivities over a broad range of human neoplasms. A major problem in the acceptance of this technique has been the question of the relationship between the cultured cells and the original patient tumor, i.e., whether the colonies that develop derive from the neoplasm or from some other cell type within the initial cell population. A study of the ultrastructural morphology of the cultured cells vs. patient tumor has therefore been undertaken to resolve this question. Direct correlation was assured by division of a common tumor mass at surgical resection, one biopsy being fixed for TEM studies, the second being rapidly transported to the laboratory for culture.

Structural analysis of the photosynthetic membrane from Ectothiorhodospira halochloris

1983 ◽  
Vol 41 ◽  
pp. 740-741
Author(s):  
H. Engelhardt ◽  
R. Guckenberger ◽  
W. Baumeister
Keyword(s):  
Lattice Structure ◽  
Bacterial Species ◽  
Hexagonal Lattice ◽  
Reaction Centre ◽  
Photosynthetic Membrane ◽  
Rhodopseudomonas Viridis ◽  
Photosynthetic Membranes ◽  
Ectothiorhodospira Halochloris ◽  
Main Components

Bacterial photosynthetic membranes contain, apart from lipids and electron transport components, reaction centre (RC) and light harvesting (LH) polypeptides as the main components. The RC-LH complexes in Rhodopseudomonas viridis membranes are known since quite seme time to form a hexagonal lattice structure in vivo; hence this membrane attracted the particular attention of electron microscopists. Contrary to previous claims in the literature we found, however, that 2-D periodically organized photosynthetic membranes are not a unique feature of Rhodopseudomonas viridis. At least five bacterial species, all bacteriophyll b - containing, possess membranes with the RC-LH complexes regularly arrayed. All these membranes appear to have a similar lattice structure and fine-morphology. The lattice spacings of the Ectothiorhodospira haloohloris, Ectothiorhodospira abdelmalekii and Rhodopseudomonas viridis membranes are close to 13 nm, those of Thiocapsa pfennigii and Rhodopseudomonas sulfoviridis are slightly smaller (∼12.5 nm).

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