The BRCT Domain of PARP1 Binds Intact DNA and Mediates Intrastrand Transfer

2021 ◽  
Author(s):  
Johannes Rudolph ◽  
Uma M. Muthurajan ◽  
Megan Palacio ◽  
Jyothi Mahadevan ◽  
Genevieve Roberts ◽  
...  
Keyword(s):  
Brct Domain

Biophysical characterization of the interaction between FAAP20-UBZ4 domain and Rev1-BRCT domain

FEBS Letters ◽  
2015 ◽  
Vol 589 (20PartB) ◽  
pp. 3037-3043 ◽  
Author(s):  
Kyungeun Lim ◽  
Mi-Kyung Lee ◽  
Phuong T.M. Duong ◽  
Dinan Liu ◽  
Sieun Sung ◽  
...  
Keyword(s):  
Brct Domain ◽  
Biophysical Characterization

scholarly journals Detection of a novel mutation in exon 20 of the BRCA1 gene

2013 ◽  
Vol 18 (4) ◽  
Author(s):  
Abhijit Chakraborty ◽  
Atul Katarkar ◽  
Keya Chaudhuri ◽  
Ashis Mukhopadhyay ◽  
Jayasri Basak
Keyword(s):  
Breast Cancer ◽  
Tumor Suppressor ◽  
Hereditary Breast Cancer ◽  
Tumor Suppressor Genes ◽  
Novel Mutation ◽  
Mutation Screening ◽  
Brct Domain ◽  
Globular Domain ◽  
Suppressor Genes ◽  
Exon 20

AbstractHereditary breast cancer constitutes 5–10% of all breast cancer cases. Inherited mutations in the BRCA1 and BRCA2 tumor-suppressor genes account for the majority of hereditary breast cancer cases. The BRCA1 C-terminal region (BRCT) has a functional duplicated globular domain, which helps with DNA damage repair and cell cycle checkpoint protein control. More than 100 distinct BRCA1 missense variants with structural and functional effects have been documented within the BRCT domain. Interpreting the results of mutation screening of tumor-suppressor genes that can have high-risk susceptibility mutations is increasingly important in clinical practice. This study includes a novel mutation, p.His1746 Pro (c.5237A>C), which was found in BRCA1 exon 20 of a breast cancer patient. In silico analysis suggests that this mutation could alter the stability and orientation of the BRCT domain and the differential binding of the BACH1 substrate.

scholarly journals Efficient Single-Strand Break Repair Requires Binding to Both Poly(ADP-Ribose) and DNA by the Central BRCT Domain of XRCC1

Cell Reports ◽  
2019 ◽  
Vol 26 (3) ◽  
pp. 573-581.e5 ◽  
Author(s):  
Luis M. Polo ◽  
Yingqi Xu ◽  
Peter Hornyak ◽  
Fernando Garces ◽  
Zhihong Zeng ◽  
...  
Keyword(s):  
Strand Break ◽  
Single Strand ◽  
Brct Domain ◽  
Single Strand Break ◽  
Single Strand Break Repair ◽  
Break Repair

scholarly journals BRCT Domain-Containing Protein PTIP Is Essential for Progression through Mitosis

2003 ◽  
Vol 23 (5) ◽  
pp. 1666-1673 ◽  
Author(s):  
Eun Ah Cho ◽  
Marc J. Prindle ◽  
Gregory R. Dressler
Keyword(s):  
Cell Proliferation ◽  
Dna Repair ◽  
Cell Cycle Control ◽  
Embryonic Stem ◽  
Essential Element ◽  
Brct Domain ◽  
Transactivation Domain ◽  
Mouse Development ◽  
Nuclear Condensation ◽  
Interacting Protein

ABSTRACT The Pax transactivation domain-interacting protein (PTIP) is a large nuclear protein with multiple BRCT domains that was identified on the basis of its interaction with transcription factors of the Pax and Smad families. To address the function of PTIP during mouse development, we generated a constitutive null allele. Homozygous PTIP mutants are developmentally retarded, disorganized, and embryonic lethal by day 9.5 of embryonic development (E9.5). PTIP mutant cells appear to replicate DNA but show reduced levels of mitosis and widespread cell death by E8.5. DNA damage appears to precede nuclear condensation at E7.5, suggesting a defect in DNA repair. Neither embryonic fibroblast nor embryonic stem cells from PTIP mutants proliferate in culture, suggesting a fundamental defect in cell proliferation. Trophoblast cells from PTIP mutants are more sensitive to DNA-damaging agents. Condensation of chromatin and expression of phospho-histone H3 are also affected in PTIP mutants, and this may underlie the inability of PTIP mutants to progress through mitosis. Given the role of BRCT domain proteins in DNA repair and cell cycle control, we propose that PTIP is an essential element of the cell proliferation machinery, perhaps by functioning in the DNA repair pathways.

scholarly journals Regulation of E2F1 by BRCT Domain-Containing Protein TopBP1

2003 ◽  
Vol 23 (9) ◽  
pp. 3287-3304 ◽  
Author(s):  
Kang Liu ◽  
Fang-Tsyr Lin ◽  
J. Michael Ruppert ◽  
Weei-Chin Lin
Keyword(s):  
Dna Damage ◽  
Cell Cycle Progression ◽  
Specific Interaction ◽  
Dna Damage Checkpoint ◽  
Brct Domain ◽  
Replication Forks ◽  
Direct Role ◽  
Dna Topoisomerase ◽  
E2f Transcription Factor ◽  
Stalled Replication Forks

ABSTRACT The E2F transcription factor integrates cellular signals and coordinates cell cycle progression. Our prior studies demonstrated selective induction and stabilization of E2F1 through ATM-dependent phosphorylation in response to DNA damage. Here we report that DNA topoisomerase IIβ binding protein 1 (TopBP1) regulates E2F1 during DNA damage. TopBP1 contains eight BRCT (BRCA1 carboxyl-terminal) motifs and upon DNA damage is recruited to stalled replication forks, where it participates in a DNA damage checkpoint. Here we demonstrated an interaction between TopBP1 and E2F1. The interaction depended on the amino terminus of E2F1 and the sixth BRCT domain of TopBP1. It was specific to E2F1 and was not observed in E2F2, E2F3, or E2F4. This interaction was induced by DNA damage and phosphorylation of E2F1 by ATM. Through this interaction, TopBP1 repressed multiple activities of E2F1, including transcriptional activity, induction of S-phase entry, and apoptosis. Furthermore, TopBP1 relocalized E2F1 from diffuse nuclear distribution to discrete punctate nuclear foci, where E2F1 colocalized with TopBP1 and BRCA1. Thus, the specific interaction between TopBP1 and E2F1 during DNA damage inhibits the known E2F1 activities but recruits E2F1 to a BRCA1-containing repair complex, suggesting a direct role of E2F1 in DNA damage checkpoint/repair at stalled replication forks.

scholarly journals The Sir4 H‐ BRCT domain interacts with phospho‐proteins to sequester and repress yeast heterochromatin

2019 ◽  
Vol 38 (20) ◽  
Author(s):  
Ishan Deshpande ◽  
Jeremy J Keusch ◽  
Kiran Challa ◽  
Vytautas Iesmantavicius ◽  
Susan M Gasser ◽  
...  
Keyword(s):  
Brct Domain
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